Advanced search
1 file | 944.89 KB Add to list

Enrichment of hydrogen-oxidizing bacteria from high-temperature and high-salinity environments

Author
Organization
Abstract
There is an urgent need for sustainable protein supply routes with low environmental footprint. Recently, the use of hydrogen-oxidizing bacteria (HOB) as a platform for high-quality microbial protein (MP) production has regained interest. This study aims to investigate the added value of using conditions such as salt and temperature to steer HOB communities to lower diversities, while maintaining a high protein content and a high-quality amino acid profile. Pressure drop and hydrogen consumption were measured for 56 days to evaluate autotrophy of a total of six communities in serum flasks. Of the six communities, four were enriched under saline (0.0, 0.25, 0.5, and 1.0 mol NaCl liter(-1)) and two under thermophilic conditions (65 degrees C). Five communities enriched for HOB were subsequently cultivated in continuously stirred reactors under the same conditions to evaluate their potential as microbial protein producers. The protein percentages ranged from 41 to 80%. The highest protein content was obtained for the thermophilic enrichments. Amino acid profiles were comparable to protein sources commonly used for feed purposes. Members of the genus Achromobacter were found to dominate the saline enrichments, while members of the genus Hydrogenibacillus were found to dominate the thermophilic enrichments. Here, we show that enriching for HOB while steering the community toward low diversity and maintaining a high-quality protein content can be successfully achieved, in both saline and thermophilic conditions. IMPORTANCE: Alternative feed and food supply chains are required to decrease water and land use. HOB offer a promising substitute for traditional agricultural practice to produce microbial protein (MP) from residual materials and renewable energy. To safeguard product stability, the composition of the HOB community should be controlled. Defining strategies to maintain the stability of the communities is therefore key for optimization purposes. In this study, we use salt and temperature as independent conditions to stabilize the composition of the HOB communities. Based on the results presented, we conclude that HOB communities can be steered to have low diversity using the presented conditions while producing a desirable protein content with a valuable amino acid profile.
Keywords
hydrogen oxidizing bacteria, HOB, enrichment, high salinity, high temperature, microbial protein, GRADIENT GEL-ELECTROPHORESIS, ALCALIGENES-EUTROPHUS, BACILLUS-SCHLEGELII, PROTEIN, WATER, EXTREMOPHILES, INACTIVATION, REGULATIONS, COMMUNITIES, SEQUENCE

Downloads

  • (...).pdf
    • full text (Published version)
    • |
    • UGent only
    • |
    • PDF
    • |
    • 944.89 KB

Citation

Please use this url to cite or link to this publication:

MLA
Goncalves Barbosa, Vanessa Raquel, et al. “Enrichment of Hydrogen-Oxidizing Bacteria from High-Temperature and High-Salinity Environments.” APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 87, no. 4, 2021, doi:10.1128/AEM.02439-20.
APA
Goncalves Barbosa, V. R., van Veelen, H. P. J., Pinheiro, V., Sleutels, T., Verstraete, W., & Boon, N. (2021). Enrichment of hydrogen-oxidizing bacteria from high-temperature and high-salinity environments. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 87(4). https://doi.org/10.1128/AEM.02439-20
Chicago author-date
Goncalves Barbosa, Vanessa Raquel, H. Pieter J. van Veelen, Vanessa Pinheiro, Tom Sleutels, Willy Verstraete, and Nico Boon. 2021. “Enrichment of Hydrogen-Oxidizing Bacteria from High-Temperature and High-Salinity Environments.” APPLIED AND ENVIRONMENTAL MICROBIOLOGY 87 (4). https://doi.org/10.1128/AEM.02439-20.
Chicago author-date (all authors)
Goncalves Barbosa, Vanessa Raquel, H. Pieter J. van Veelen, Vanessa Pinheiro, Tom Sleutels, Willy Verstraete, and Nico Boon. 2021. “Enrichment of Hydrogen-Oxidizing Bacteria from High-Temperature and High-Salinity Environments.” APPLIED AND ENVIRONMENTAL MICROBIOLOGY 87 (4). doi:10.1128/AEM.02439-20.
Vancouver
1.
Goncalves Barbosa VR, van Veelen HPJ, Pinheiro V, Sleutels T, Verstraete W, Boon N. Enrichment of hydrogen-oxidizing bacteria from high-temperature and high-salinity environments. APPLIED AND ENVIRONMENTAL MICROBIOLOGY. 2021;87(4).
IEEE
[1]
V. R. Goncalves Barbosa, H. P. J. van Veelen, V. Pinheiro, T. Sleutels, W. Verstraete, and N. Boon, “Enrichment of hydrogen-oxidizing bacteria from high-temperature and high-salinity environments,” APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 87, no. 4, 2021.
@article{8700863,
  abstract     = {{There is an urgent need for sustainable protein supply routes with low environmental footprint. Recently, the use of hydrogen-oxidizing bacteria (HOB) as a platform for high-quality microbial protein (MP) production has regained interest. This study aims to investigate the added value of using conditions such as salt and temperature to steer HOB communities to lower diversities, while maintaining a high protein content and a high-quality amino acid profile. Pressure drop and hydrogen consumption were measured for 56 days to evaluate autotrophy of a total of six communities in serum flasks. Of the six communities, four were enriched under saline (0.0, 0.25, 0.5, and 1.0 mol NaCl liter(-1)) and two under thermophilic conditions (65 degrees C). Five communities enriched for HOB were subsequently cultivated in continuously stirred reactors under the same conditions to evaluate their potential as microbial protein producers. The protein percentages ranged from 41 to 80%. The highest protein content was obtained for the thermophilic enrichments. Amino acid profiles were comparable to protein sources commonly used for feed purposes. Members of the genus Achromobacter were found to dominate the saline enrichments, while members of the genus Hydrogenibacillus were found to dominate the thermophilic enrichments. Here, we show that enriching for HOB while steering the community toward low diversity and maintaining a high-quality protein content can be successfully achieved, in both saline and thermophilic conditions. 

IMPORTANCE: Alternative feed and food supply chains are required to decrease water and land use. HOB offer a promising substitute for traditional agricultural practice to produce microbial protein (MP) from residual materials and renewable energy. To safeguard product stability, the composition of the HOB community should be controlled. Defining strategies to maintain the stability of the communities is therefore key for optimization purposes. In this study, we use salt and temperature as independent conditions to stabilize the composition of the HOB communities. Based on the results presented, we conclude that HOB communities can be steered to have low diversity using the presented conditions while producing a desirable protein content with a valuable amino acid profile.}},
  articleno    = {{e02439-20}},
  author       = {{G. Barbosa, Raquel and van Veelen, H. Pieter J. and Pinheiro, Vanessa and Sleutels, Tom and Verstraete, Willy and Boon, Nico}},
  issn         = {{0099-2240}},
  journal      = {{APPLIED AND ENVIRONMENTAL MICROBIOLOGY}},
  keywords     = {{hydrogen oxidizing bacteria,HOB,enrichment,high salinity,high temperature,microbial protein,GRADIENT GEL-ELECTROPHORESIS,ALCALIGENES-EUTROPHUS,BACILLUS-SCHLEGELII,PROTEIN,WATER,EXTREMOPHILES,INACTIVATION,REGULATIONS,COMMUNITIES,SEQUENCE}},
  language     = {{eng}},
  number       = {{4}},
  pages        = {{11}},
  title        = {{Enrichment of hydrogen-oxidizing bacteria from high-temperature and high-salinity environments}},
  url          = {{http://dx.doi.org/10.1128/AEM.02439-20}},
  volume       = {{87}},
  year         = {{2021}},
}

Altmetric
View in Altmetric
Web of Science
Times cited: