Advanced search
1 file | 2.94 MB Add to list

Morphological and molecular characterization of Pratylenchus species from Yam (Dioscorea spp.) in West Africa

Author
Organization
Abstract
The root-lesion nematodes (RLN), Pratylenchus spp., are among the major plant-parasitic nematodes affecting yam (Dioscorea spp.) production in West Africa. The distribution and diversity of RLN species associated with yam was investigated through a soil and tuber survey of the main producing areas in Nigeria and Ghana. Pratylenchus spp. were detected in the yam rhizosphere in 59% of 81 soil samples from Ghana and 39% of 114 soil samples from Nigeria. Pratylenchus spp. were detected in 24 of 400 tubers examined, in combination with root-knot nematodes (Meloidogyne spp.) and their associated damage of galls and crazy roots (79%), and with yam nematode (Scutellonema bradys) and their associated damage of dry rot (17%), although no specific additional symptoms were observed for Pratylenchus spp. Species of Pratylenchus were identified by their morphological features and by sequences of the D2-D3 region of the 28 S rDNA gene and the mitochondrial cytochrome oxidase I gene (COI). Pratylenchus brachyurus was the most frequent RLN species in both the rhizosphere and tubers of yam. Pratylenchus hexincisus was recovered from one tuber collected in Nigeria. While further investigations are required to establish the host status of yam for this nematode, this appears to be the first record of P. hexincisus on yam. The present taxonomical status of P. scribneri and P. hexincisus is discussed.
Keywords
Agronomy and Crop Science, COI, D2-D3, Dioscorea, DNA, Ghana, Identification, Molecular, Morphology, Morphometrics, Nigeria, Phylogeny, Pratylenchus, Root-lesion nematodes, Taxonomy, West Africa, Yam, N. SP NEMATODA, SCUTELLONEMA-BRADYS, PREVALENCE, BRACHYURUS, FRAMEWORK, DISEASE, COFFEAE, NIGERIA

Downloads

  • 2021 Pratylenchus from Yam Kolombia et al jofnem-2020-126.pdf
    • full text (Published version)
    • |
    • open access
    • |
    • PDF
    • |
    • 2.94 MB

Citation

Please use this url to cite or link to this publication:

MLA
Kolombia, Yao A., et al. “Morphological and Molecular Characterization of Pratylenchus Species from Yam (Dioscorea Spp.) in West Africa.” JOURNAL OF NEMATOLOGY, vol. 52, 2021, doi:10.21307/jofnem-2020-126.
APA
Kolombia, Y. A., Ogundero, O., Olajide, E. O., Viaene, N., Kumar, P. L., Coyne, D. L., & Bert, W. (2021). Morphological and molecular characterization of Pratylenchus species from Yam (Dioscorea spp.) in West Africa. JOURNAL OF NEMATOLOGY, 52. https://doi.org/10.21307/jofnem-2020-126
Chicago author-date
Kolombia, Yao A., Oluwadamilola Ogundero, Emmanuel Olorunfemi Olajide, Nicole Viaene, P. Lava Kumar, Danny L. Coyne, and Wim Bert. 2021. “Morphological and Molecular Characterization of Pratylenchus Species from Yam (Dioscorea Spp.) in West Africa.” JOURNAL OF NEMATOLOGY 52. https://doi.org/10.21307/jofnem-2020-126.
Chicago author-date (all authors)
Kolombia, Yao A., Oluwadamilola Ogundero, Emmanuel Olorunfemi Olajide, Nicole Viaene, P. Lava Kumar, Danny L. Coyne, and Wim Bert. 2021. “Morphological and Molecular Characterization of Pratylenchus Species from Yam (Dioscorea Spp.) in West Africa.” JOURNAL OF NEMATOLOGY 52. doi:10.21307/jofnem-2020-126.
Vancouver
1.
Kolombia YA, Ogundero O, Olajide EO, Viaene N, Kumar PL, Coyne DL, et al. Morphological and molecular characterization of Pratylenchus species from Yam (Dioscorea spp.) in West Africa. JOURNAL OF NEMATOLOGY. 2021;52.
IEEE
[1]
Y. A. Kolombia et al., “Morphological and molecular characterization of Pratylenchus species from Yam (Dioscorea spp.) in West Africa,” JOURNAL OF NEMATOLOGY, vol. 52, 2021.
@article{8700338,
  abstract     = {{The root-lesion nematodes (RLN), Pratylenchus spp., are among the major plant-parasitic nematodes affecting yam (Dioscorea spp.) production in West Africa. The distribution and diversity of RLN species associated with yam was investigated through a soil and tuber survey of the main producing areas in Nigeria and Ghana. Pratylenchus spp. were detected in the yam rhizosphere in 59% of 81 soil samples from Ghana and 39% of 114 soil samples from Nigeria. Pratylenchus spp. were detected in 24 of 400 tubers examined, in combination with root-knot nematodes (Meloidogyne spp.) and their associated damage of galls and crazy roots (79%), and with yam nematode (Scutellonema bradys) and their associated damage of dry rot (17%), although no specific additional symptoms were observed for Pratylenchus spp. Species of Pratylenchus were identified by their morphological features and by sequences of the D2-D3 region of the 28 S rDNA gene and the mitochondrial cytochrome oxidase I gene (COI). Pratylenchus brachyurus was the most frequent RLN species in both the rhizosphere and tubers of yam. Pratylenchus hexincisus was recovered from one tuber collected in Nigeria. While further investigations are required to establish the host status of yam for this nematode, this appears to be the first record of P. hexincisus on yam. The present taxonomical status of P. scribneri and P. hexincisus is discussed.}},
  articleno    = {{e2020-126}},
  author       = {{Kolombia, Yao A. and Ogundero, Oluwadamilola and Olajide, Emmanuel Olorunfemi and Viaene, Nicole and Kumar, P. Lava and Coyne, Danny L. and Bert, Wim}},
  issn         = {{0022-300X}},
  journal      = {{JOURNAL OF NEMATOLOGY}},
  keywords     = {{Agronomy and Crop Science,COI,D2-D3,Dioscorea,DNA,Ghana,Identification,Molecular,Morphology,Morphometrics,Nigeria,Phylogeny,Pratylenchus,Root-lesion nematodes,Taxonomy,West Africa,Yam,N. SP NEMATODA,SCUTELLONEMA-BRADYS,PREVALENCE,BRACHYURUS,FRAMEWORK,DISEASE,COFFEAE,NIGERIA}},
  language     = {{eng}},
  pages        = {{25}},
  title        = {{Morphological and molecular characterization of Pratylenchus species from Yam (Dioscorea spp.) in West Africa}},
  url          = {{http://dx.doi.org/10.21307/jofnem-2020-126}},
  volume       = {{52}},
  year         = {{2021}},
}

Altmetric
View in Altmetric
Web of Science
Times cited: