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Distinct cellular strategies determine sensitivity to mild drought of Arabidopsis natural accessions

Ying Chen (UGent) , Marieke Dubois (UGent) , Mattias Vermeersch (UGent) , Dirk Inzé (UGent) and Hannes Vanhaeren (UGent)
(2021) PLANT PHYSIOLOGY. 186(2). p.1171-1185
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Abstract
The world-wide distribution of Arabidopsis (Arabidopsis thaliana) accessions imposes different types of evolutionary pressures, which contributes to various responses of these accessions to environmental stresses. Responses to drought stress have mostly been studied in the Columbia accession, which is predominantly used in plant research. However, the reactions to drought stress are complex and our understanding of the responses that contribute to maintaining plant growth during mild drought is very limited. Here, we studied the mechanisms with which natural accessions react to mild drought at a physiological and molecular level during early leaf development. We documented variations in mild drought responses among natural accessions and used transcriptome sequencing of a drought-sensitive accession, ICE163, and a drought-insensitive accession, Yeg-1, to gain insights into the mechanisms underlying this discrepancy. This revealed that ICE163 preferentially induces jasmonate- and anthocyanin-related pathways, which are beneficial in biotic stress defense, whereas Yeg-1 has a more pronounced activation of abscisic acid signaling, the classical abiotic stress response. Related physiological traits, including content of proline, anthocyanins and reactive oxygen species, stomatal closure and cellular leaf parameters, were investigated and linked to the transcriptional responses. We can conclude that most of these processes constitute general drought response mechanisms that are regulated similarly in drought-insensitive and -sensitive accessions. However, the capacity to close stomata and maintain cell expansion under mild drought appeared to be major factors that allow to better sustain leaf growth under mild drought.
Keywords
Plant Science, Genetics, Physiology, INDUCED STOMATAL CLOSURE, WATER-USE EFFICIENCY, STRESS TOLERANCE, PROLINE ACCUMULATION, LEAF DEVELOPMENT, GROWTH, THALIANA, GENE, RESPONSES, ABA

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Citation

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MLA
Chen, Ying, et al. “Distinct Cellular Strategies Determine Sensitivity to Mild Drought of Arabidopsis Natural Accessions.” PLANT PHYSIOLOGY, vol. 186, no. 2, 2021, pp. 1171–85, doi:10.1093/plphys/kiab115.
APA
Chen, Y., Dubois, M., Vermeersch, M., Inzé, D., & Vanhaeren, H. (2021). Distinct cellular strategies determine sensitivity to mild drought of Arabidopsis natural accessions. PLANT PHYSIOLOGY, 186(2), 1171–1185. https://doi.org/10.1093/plphys/kiab115
Chicago author-date
Chen, Ying, Marieke Dubois, Mattias Vermeersch, Dirk Inzé, and Hannes Vanhaeren. 2021. “Distinct Cellular Strategies Determine Sensitivity to Mild Drought of Arabidopsis Natural Accessions.” PLANT PHYSIOLOGY 186 (2): 1171–85. https://doi.org/10.1093/plphys/kiab115.
Chicago author-date (all authors)
Chen, Ying, Marieke Dubois, Mattias Vermeersch, Dirk Inzé, and Hannes Vanhaeren. 2021. “Distinct Cellular Strategies Determine Sensitivity to Mild Drought of Arabidopsis Natural Accessions.” PLANT PHYSIOLOGY 186 (2): 1171–1185. doi:10.1093/plphys/kiab115.
Vancouver
1.
Chen Y, Dubois M, Vermeersch M, Inzé D, Vanhaeren H. Distinct cellular strategies determine sensitivity to mild drought of Arabidopsis natural accessions. PLANT PHYSIOLOGY. 2021;186(2):1171–85.
IEEE
[1]
Y. Chen, M. Dubois, M. Vermeersch, D. Inzé, and H. Vanhaeren, “Distinct cellular strategies determine sensitivity to mild drought of Arabidopsis natural accessions,” PLANT PHYSIOLOGY, vol. 186, no. 2, pp. 1171–1185, 2021.
@article{8700100,
  abstract     = {{The world-wide distribution of Arabidopsis (Arabidopsis thaliana) accessions imposes different types of evolutionary pressures, which contributes to various responses of these accessions to environmental stresses. Responses to drought stress have mostly been studied in the Columbia accession, which is predominantly used in plant research. However, the reactions to drought stress are complex and our understanding of the responses that contribute to maintaining plant growth during mild drought is very limited. Here, we studied the mechanisms with which natural accessions react to mild drought at a physiological and molecular level during early leaf development. We documented variations in mild drought responses among natural accessions and used transcriptome sequencing of a drought-sensitive accession, ICE163, and a drought-insensitive accession, Yeg-1, to gain insights into the mechanisms underlying this discrepancy. This revealed that ICE163 preferentially induces jasmonate- and anthocyanin-related pathways, which are beneficial in biotic stress defense, whereas Yeg-1 has a more pronounced activation of abscisic acid signaling, the classical abiotic stress response. Related physiological traits, including content of proline, anthocyanins and reactive oxygen species, stomatal closure and cellular leaf parameters, were investigated and linked to the transcriptional responses. We can conclude that most of these processes constitute general drought response mechanisms that are regulated similarly in drought-insensitive and -sensitive accessions. However, the capacity to close stomata and maintain cell expansion under mild drought appeared to be major factors that allow to better sustain leaf growth under mild drought.}},
  author       = {{Chen, Ying and Dubois, Marieke and Vermeersch, Mattias and Inzé, Dirk and Vanhaeren, Hannes}},
  issn         = {{1532-2548}},
  journal      = {{PLANT PHYSIOLOGY}},
  keywords     = {{Plant Science,Genetics,Physiology,INDUCED STOMATAL CLOSURE,WATER-USE EFFICIENCY,STRESS TOLERANCE,PROLINE ACCUMULATION,LEAF DEVELOPMENT,GROWTH,THALIANA,GENE,RESPONSES,ABA}},
  language     = {{eng}},
  number       = {{2}},
  pages        = {{1171--1185}},
  title        = {{Distinct cellular strategies determine sensitivity to mild drought of Arabidopsis natural accessions}},
  url          = {{http://dx.doi.org/10.1093/plphys/kiab115}},
  volume       = {{186}},
  year         = {{2021}},
}

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