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AGC kinases and MAB4/MEL proteins maintain PIN polarity by limiting lateral diffusion in plant cells

(2021) CURRENT BIOLOGY. 31(9). p.1918-1930
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Abstract
Polar subcellular localization of the PIN exporters of the phytohormone auxin is a key determinant of directional, intercellular auxin transport and thus a central topic of both plant cell and developmental biology. Arabidopsis mutants lacking PID, a kinase that phosphorylates PINs, or the MAB4/MEL proteins of unknown molecular function display PIN polarity defects and phenocopy pin mutants, but mechanistic insights into how these factors convey PIN polarity are missing. Here, by combining protein biochemistry with quantitative live-cell imaging, we demonstrate that PINs, MAB4/MELs, and AGC kinases interact in the same complex at the plasma membrane. MAB4/MELs are recruited to the plasma membrane by the PINs and in concert with the AGC kinases maintain PIN polarity through limiting lateral diffusion-based escape of PINs from the polar domain. The PIN-MAB4/MEL-PID protein complex has self-reinforcing properties thanks to positive feedback between AGC kinase-mediated PIN phosphorylation and MAB4/MEL recruitment. We thus uncover the molecular mechanism by which AGC kinases and MAB4/MEL proteins regulate PIN localization and plant development.
Keywords
Arabidopsis, cell polarity, lateral diffusion, plant development, polar auxin transport, positive feedback, protein phosphorylation, DEPENDENT AUXIN TRANSPORT, SYMMETRY-BREAKING, ARABIDOPSIS, PHOSPHORYLATION, ENDOCYTOSIS, TRAFFICKING, ORGANOGENESIS, PHOTOTROPISM, POLARIZATION, GRADIENTS

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MLA
Glanc, Matous, et al. “AGC Kinases and MAB4/MEL Proteins Maintain PIN Polarity by Limiting Lateral Diffusion in Plant Cells.” CURRENT BIOLOGY, vol. 31, no. 9, 2021, pp. 1918–30, doi:10.1016/j.cub.2021.02.028.
APA
Glanc, M., Van Gelderen, K., Hoermayer, L., Tan, S., Naramoto, S., Zhang, X., … Friml, J. (2021). AGC kinases and MAB4/MEL proteins maintain PIN polarity by limiting lateral diffusion in plant cells. CURRENT BIOLOGY, 31(9), 1918–1930. https://doi.org/10.1016/j.cub.2021.02.028
Chicago author-date
Glanc, Matous, Kasper Van Gelderen, Lukas Hoermayer, Shutang Tan, Satoshi Naramoto, Xixi Zhang, David Domjan, et al. 2021. “AGC Kinases and MAB4/MEL Proteins Maintain PIN Polarity by Limiting Lateral Diffusion in Plant Cells.” CURRENT BIOLOGY 31 (9): 1918–30. https://doi.org/10.1016/j.cub.2021.02.028.
Chicago author-date (all authors)
Glanc, Matous, Kasper Van Gelderen, Lukas Hoermayer, Shutang Tan, Satoshi Naramoto, Xixi Zhang, David Domjan, Ludmila Včelařová, Robert Hauschild, Alexander Johnson, Edward de Koning, Maritza van Dop, Eike Rademacher, Stef Janson, Xiaoyu Wei, Gergely Molnár, Matyáš Fendrych, Bert De Rybel, Remko Offringa, and Jiří Friml. 2021. “AGC Kinases and MAB4/MEL Proteins Maintain PIN Polarity by Limiting Lateral Diffusion in Plant Cells.” CURRENT BIOLOGY 31 (9): 1918–1930. doi:10.1016/j.cub.2021.02.028.
Vancouver
1.
Glanc M, Van Gelderen K, Hoermayer L, Tan S, Naramoto S, Zhang X, et al. AGC kinases and MAB4/MEL proteins maintain PIN polarity by limiting lateral diffusion in plant cells. CURRENT BIOLOGY. 2021;31(9):1918–30.
IEEE
[1]
M. Glanc et al., “AGC kinases and MAB4/MEL proteins maintain PIN polarity by limiting lateral diffusion in plant cells,” CURRENT BIOLOGY, vol. 31, no. 9, pp. 1918–1930, 2021.
@article{8700085,
  abstract     = {{Polar subcellular localization of the PIN exporters of the phytohormone auxin is a key determinant of directional, intercellular auxin transport and thus a central topic of both plant cell and developmental biology. Arabidopsis mutants lacking PID, a kinase that phosphorylates PINs, or the MAB4/MEL proteins of unknown molecular function display PIN polarity defects and phenocopy pin mutants, but mechanistic insights into how these factors convey PIN polarity are missing. Here, by combining protein biochemistry with quantitative live-cell imaging, we demonstrate that PINs, MAB4/MELs, and AGC kinases interact in the same complex at the plasma membrane. MAB4/MELs are recruited to the plasma membrane by the PINs and in concert with the AGC kinases maintain PIN polarity through limiting lateral diffusion-based escape of PINs from the polar domain. The PIN-MAB4/MEL-PID protein complex has self-reinforcing properties thanks to positive feedback between AGC kinase-mediated PIN phosphorylation and MAB4/MEL recruitment. We thus uncover the molecular mechanism by which AGC kinases and MAB4/MEL proteins regulate PIN localization and plant development.}},
  author       = {{Glanc, Matous and Van Gelderen, Kasper and Hoermayer, Lukas and Tan, Shutang and Naramoto, Satoshi and Zhang, Xixi and Domjan, David and Včelařová, Ludmila and Hauschild, Robert and Johnson, Alexander and de Koning, Edward and van Dop, Maritza and Rademacher, Eike and Janson, Stef and Wei, Xiaoyu and Molnár, Gergely and Fendrych, Matyáš and De Rybel, Bert and Offringa, Remko and Friml, Jiří}},
  issn         = {{0960-9822}},
  journal      = {{CURRENT BIOLOGY}},
  keywords     = {{Arabidopsis,cell polarity,lateral diffusion,plant development,polar auxin transport,positive feedback,protein phosphorylation,DEPENDENT AUXIN TRANSPORT,SYMMETRY-BREAKING,ARABIDOPSIS,PHOSPHORYLATION,ENDOCYTOSIS,TRAFFICKING,ORGANOGENESIS,PHOTOTROPISM,POLARIZATION,GRADIENTS}},
  language     = {{eng}},
  number       = {{9}},
  pages        = {{1918--1930}},
  title        = {{AGC kinases and MAB4/MEL proteins maintain PIN polarity by limiting lateral diffusion in plant cells}},
  url          = {{http://dx.doi.org/10.1016/j.cub.2021.02.028}},
  volume       = {{31}},
  year         = {{2021}},
}

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