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The Arabidopsis mediator complex subunit 8 regulates oxidative stress responses

(2021) PLANT CELL. 33(6). p.2032-2057
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Abstract
Signaling events triggered by hydrogen peroxide (H2O2) regulate plant growth and defense by orchestrating a genome-wide transcriptional reprogramming. However, the specific mechanisms that govern H2O2-dependent gene expression are still poorly understood. Here we identify the Arabidopsis Mediator complex subunit MED8 as a regulator of H2O2 responses. The introduction of the med8 mutation in a constitutive oxidative stress genetic background (catalase-deficient, cat2) was associated with enhanced activation of the salicylic acid pathway and accelerated cell death. Interestingly, med8 seedlings were more tolerant to oxidative stress generated by the herbicide methyl viologen and exhibited transcriptional hyperactivation of defense signaling, in particular salicylic acid- and jasmonic acid-related pathways. The med8-triggered tolerance to methyl viologen was manipulated by introduction of secondary mutations in salicylic acid and jasmonic acid pathways. In addition, analysis of the Mediator interactome revealed interactions with components involved in mRNA processing and microRNA biogenesis, hence expanding the role of Mediator beyond transcription. Notably, MED8 interacted with the transcriptional regulator NEGATIVE ON TATA-LESS, NOT2, to control the expression of H2O2-inducible genes and stress responses. Our work establishes MED8 as a component regulating oxidative stress responses and demonstrates that it acts as a negative regulator of H2O2 -driven activation of defense gene expression.
Keywords
ICS1, Mediator complex, NOT2, catalase, hydrogen peroxide, oxidative signaling, transcriptome, DIFFERENTIAL EXPRESSION ANALYSIS, DEFENSE GENE-EXPRESSION, TRANSCRIPTION FACTOR, HYDROGEN-PEROXIDE, SALICYLIC-ACID, KEY REGULATOR, ORGAN SIZE, PATHOGENESIS RESPONSES, DAY LENGTH, HIGH LIGHT

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Citation

Please use this url to cite or link to this publication:

MLA
He, Huaming, et al. “The Arabidopsis Mediator Complex Subunit 8 Regulates Oxidative Stress Responses.” PLANT CELL, vol. 33, no. 6, 2021, pp. 2032–57, doi:10.1093/plcell/koab079.
APA
He, H., Denecker, J., Van Der Kelen, K., Willems, P., Pottie, R., Phua, S. Y., … M’Hamdi, A. (2021). The Arabidopsis mediator complex subunit 8 regulates oxidative stress responses. PLANT CELL, 33(6), 2032–2057. https://doi.org/10.1093/plcell/koab079
Chicago author-date
He, Huaming, Jordi Denecker, Katrien Van Der Kelen, Patrick Willems, Robin Pottie, Su Yin Phua, Matthew A. Hannah, Didier Vertommen, Frank Van Breusegem, and Amna M’Hamdi. 2021. “The Arabidopsis Mediator Complex Subunit 8 Regulates Oxidative Stress Responses.” PLANT CELL 33 (6): 2032–57. https://doi.org/10.1093/plcell/koab079.
Chicago author-date (all authors)
He, Huaming, Jordi Denecker, Katrien Van Der Kelen, Patrick Willems, Robin Pottie, Su Yin Phua, Matthew A. Hannah, Didier Vertommen, Frank Van Breusegem, and Amna M’Hamdi. 2021. “The Arabidopsis Mediator Complex Subunit 8 Regulates Oxidative Stress Responses.” PLANT CELL 33 (6): 2032–2057. doi:10.1093/plcell/koab079.
Vancouver
1.
He H, Denecker J, Van Der Kelen K, Willems P, Pottie R, Phua SY, et al. The Arabidopsis mediator complex subunit 8 regulates oxidative stress responses. PLANT CELL. 2021;33(6):2032–57.
IEEE
[1]
H. He et al., “The Arabidopsis mediator complex subunit 8 regulates oxidative stress responses,” PLANT CELL, vol. 33, no. 6, pp. 2032–2057, 2021.
@article{8700063,
  abstract     = {{Signaling events triggered by hydrogen peroxide (H2O2) regulate plant growth and defense by orchestrating a genome-wide transcriptional reprogramming. However, the specific mechanisms that govern H2O2-dependent gene expression are still poorly understood. Here we identify the Arabidopsis Mediator complex subunit MED8 as a regulator of H2O2 responses. The introduction of the med8 mutation in a constitutive oxidative stress genetic background (catalase-deficient, cat2) was associated with enhanced activation of the salicylic acid pathway and accelerated cell death. Interestingly, med8 seedlings were more tolerant to oxidative stress generated by the herbicide methyl viologen and exhibited transcriptional hyperactivation of defense signaling, in particular salicylic acid- and jasmonic acid-related pathways. The med8-triggered tolerance to methyl viologen was manipulated by introduction of secondary mutations in salicylic acid and jasmonic acid pathways. In addition, analysis of the Mediator interactome revealed interactions with components involved in mRNA processing and microRNA biogenesis, hence expanding the role of Mediator beyond transcription. Notably, MED8 interacted with the transcriptional regulator NEGATIVE ON TATA-LESS, NOT2, to control the expression of H2O2-inducible genes and stress responses. Our work establishes MED8 as a component regulating oxidative stress responses and demonstrates that it acts as a negative regulator of H2O2 -driven activation of defense gene expression.}},
  author       = {{He, Huaming and Denecker, Jordi and Van Der Kelen, Katrien and Willems, Patrick and Pottie, Robin and Phua, Su Yin and Hannah, Matthew A. and Vertommen, Didier and Van Breusegem, Frank and M'Hamdi, Amna}},
  issn         = {{1040-4651}},
  journal      = {{PLANT CELL}},
  keywords     = {{ICS1,Mediator complex,NOT2,catalase,hydrogen peroxide,oxidative signaling,transcriptome,DIFFERENTIAL EXPRESSION ANALYSIS,DEFENSE GENE-EXPRESSION,TRANSCRIPTION FACTOR,HYDROGEN-PEROXIDE,SALICYLIC-ACID,KEY REGULATOR,ORGAN SIZE,PATHOGENESIS RESPONSES,DAY LENGTH,HIGH LIGHT}},
  language     = {{eng}},
  number       = {{6}},
  pages        = {{2032--2057}},
  title        = {{The Arabidopsis mediator complex subunit 8 regulates oxidative stress responses}},
  url          = {{http://dx.doi.org/10.1093/plcell/koab079}},
  volume       = {{33}},
  year         = {{2021}},
}

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