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Entomobacter blattae gen. nov., sp. nov., a new member of the Acetobacteraceae isolated from the gut of the cockroach Gromphadorhina portentosa

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Abstract
A novel bacterium designated G55GPT and pertaining to the family Acetobacteraceae was isolated from the gut of the Madagascar hissing cockroach Gromphadorhina portentosa. The Gram-negative cells were rod-shaped and non-motile. The complete 16S rRNA sequence of the strain G55GPT showed the highest pairwise similarity to Gluconacetobacter johannae CFN-Cf-55T (95.35 %), suggesting it represents a potential new genus of the family Acetobacteraceae . Phylogenetic analysis based on 16S rRNA gene and 106 orthologous housekeeping protein sequences revealed that G55GPT forms a monophyletic clade with the genus Commensalibacter , which thus far has also been isolated exclusively from insects. The G55GPT genome size was 2.70 Mbp, and the G+C content was 45.4 mol%, which is lower than most acetic acid bacteria (51–68 mol%) but comparable to Swingsia samuiensis AH83T (45.1 mol%) and higher than Commensalibacter intestini A911T (36.8 mol%). Overall genome relatedness indices based on gene and protein sequences strongly supported the assignment of G55GPT to a new genus within the family Acetobacteraceae . The percentage of conserved proteins, which is a useful metric for genus differentiation, was below 54 % when comparing G55GPT to type strains of acetic acid bacteria, thus strongly supporting our hypothesis that G55GPT is a member of a yet-undescribed genus. The fatty acid composition of G55GPT differed from that of closely related acetic acid bacteria, particularly given the presence of C19 : 1  ω9c/ω11c and the absence of C14 : 0 and C14 : 0 2-OH fatty acids. Strain G55GPT also differed in terms of metabolic features such as its ability to produce acid from d-mannitol, and its inability to produce acetic acid from ethanol or to oxidize glycerol to dihydroxyacetone. Based on the results of combined genomic, phenotypic and phylogenetic characterizations, isolate G55GPT (=LMG 31394T=DSM 111244T) is considered to represent a new species in a new genus, for which we propose the name Entomobacter blattae gen. nov., sp. nov.
Keywords
Ecology, Evolution, Behavior and Systematics, Microbiology, General Medicine, Entomobacter blattae, Acetobacteraceae, Gromphadorhina portentosa, cockroach microbiota, ACETIC-ACID BACTERIUM, ALGORITHM, SOFTWARE

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MLA
Guzman, Juan, et al. “Entomobacter Blattae Gen. Nov., Sp. Nov., a New Member of the Acetobacteraceae Isolated from the Gut of the Cockroach Gromphadorhina Portentosa.” INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, vol. 71, no. 3, 2021, doi:10.1099/ijsem.0.004666.
APA
Guzman, J., Sombolestani, A. S., Poehlein, A., Daniel, R., Cleenwerck, I., Vandamme, P., & Vilcinskas, A. (2021). Entomobacter blattae gen. nov., sp. nov., a new member of the Acetobacteraceae isolated from the gut of the cockroach Gromphadorhina portentosa. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 71(3). https://doi.org/10.1099/ijsem.0.004666
Chicago author-date
Guzman, Juan, Atena Sadat Sombolestani, Anja Poehlein, Rolf Daniel, Ilse Cleenwerck, Peter Vandamme, and Andreas Vilcinskas. 2021. “Entomobacter Blattae Gen. Nov., Sp. Nov., a New Member of the Acetobacteraceae Isolated from the Gut of the Cockroach Gromphadorhina Portentosa.” INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 71 (3). https://doi.org/10.1099/ijsem.0.004666.
Chicago author-date (all authors)
Guzman, Juan, Atena Sadat Sombolestani, Anja Poehlein, Rolf Daniel, Ilse Cleenwerck, Peter Vandamme, and Andreas Vilcinskas. 2021. “Entomobacter Blattae Gen. Nov., Sp. Nov., a New Member of the Acetobacteraceae Isolated from the Gut of the Cockroach Gromphadorhina Portentosa.” INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 71 (3). doi:10.1099/ijsem.0.004666.
Vancouver
1.
Guzman J, Sombolestani AS, Poehlein A, Daniel R, Cleenwerck I, Vandamme P, et al. Entomobacter blattae gen. nov., sp. nov., a new member of the Acetobacteraceae isolated from the gut of the cockroach Gromphadorhina portentosa. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY. 2021;71(3).
IEEE
[1]
J. Guzman et al., “Entomobacter blattae gen. nov., sp. nov., a new member of the Acetobacteraceae isolated from the gut of the cockroach Gromphadorhina portentosa,” INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, vol. 71, no. 3, 2021.
@article{8698544,
  abstract     = {{A novel bacterium designated G55GPT and pertaining to the family Acetobacteraceae was isolated from the gut of the Madagascar hissing cockroach Gromphadorhina portentosa. The Gram-negative cells were rod-shaped and non-motile. The complete 16S rRNA sequence of the strain G55GPT showed the highest pairwise similarity to Gluconacetobacter johannae CFN-Cf-55T (95.35 %), suggesting it represents a potential new genus of the family Acetobacteraceae . Phylogenetic analysis based on 16S rRNA gene and 106 orthologous housekeeping protein sequences revealed that G55GPT forms a monophyletic clade with the genus Commensalibacter , which thus far has also been isolated exclusively from insects. The G55GPT genome size was 2.70 Mbp, and the G+C content was 45.4 mol%, which is lower than most acetic acid bacteria (51–68 mol%) but comparable to Swingsia samuiensis AH83T (45.1 mol%) and higher than Commensalibacter intestini A911T (36.8 mol%). Overall genome relatedness indices based on gene and protein sequences strongly supported the assignment of G55GPT to a new genus within the family Acetobacteraceae . The percentage of conserved proteins, which is a useful metric for genus differentiation, was below 54 % when comparing G55GPT to type strains of acetic acid bacteria, thus strongly supporting our hypothesis that G55GPT is a member of a yet-undescribed genus. The fatty acid composition of G55GPT differed from that of closely related acetic acid bacteria, particularly given the presence of C19 : 1  ω9c/ω11c and the absence of C14 : 0 and C14 : 0 2-OH fatty acids. Strain G55GPT also differed in terms of metabolic features such as its ability to produce acid from d-mannitol, and its inability to produce acetic acid from ethanol or to oxidize glycerol to dihydroxyacetone. Based on the results of combined genomic, phenotypic and phylogenetic characterizations, isolate G55GPT (=LMG 31394T=DSM 111244T) is considered to represent a new species in a new genus, for which we propose the name Entomobacter blattae gen. nov., sp. nov.}},
  articleno    = {{004666}},
  author       = {{Guzman, Juan and Sombolestani, Atena Sadat and Poehlein, Anja and Daniel, Rolf and Cleenwerck, Ilse and Vandamme, Peter and Vilcinskas, Andreas}},
  issn         = {{1466-5026}},
  journal      = {{INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY}},
  keywords     = {{Ecology,Evolution,Behavior and Systematics,Microbiology,General Medicine,Entomobacter blattae,Acetobacteraceae,Gromphadorhina portentosa,cockroach microbiota,ACETIC-ACID BACTERIUM,ALGORITHM,SOFTWARE}},
  language     = {{eng}},
  number       = {{3}},
  pages        = {{13}},
  title        = {{Entomobacter blattae gen. nov., sp. nov., a new member of the Acetobacteraceae isolated from the gut of the cockroach Gromphadorhina portentosa}},
  url          = {{http://doi.org/10.1099/ijsem.0.004666}},
  volume       = {{71}},
  year         = {{2021}},
}

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