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Pandanus tectorius fruit extract promotes Hsp70 accumulation, immune-related genes expression and Vibrio parahaemolyticus tolerance in the white-leg shrimp Penaeus vannamei

Anupa Anirudhan, Victor Tosin Okomoda, Mat Taib Mimi Iryani, Yosie Andriani, Mohd Effendy Abd Wahid, Min Pau Tan, Muhd Danish-Daniel, Li Lian Wong, Tengku Sifzizul Tengku-Muhammad, Wen Jye Mok, et al.
(2021) FISH & SHELLFISH IMMUNOLOGY. 109. p.97-105
Author
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Abstract
Plants and herbal extracts are indispensable for controlling the spread of disease-causing bacteria, including those that infect aquatic organisms used in aquaculture. The use of plant or herbal extract is expected to be safe for aquatic animals and less harmful to the environment, as opposed to conventional therapeutic alternatives such as antibiotics that promote the occurrence of potential antibiotic-resistant bacteria when used improperly. The efficacy of Pandanus tectorius fruit extract in the regulation of Hsp70 expression, pro-phenoloxidase (ProPO), peroxinectin, penaeidin, crustin and transglutaminase, all immune peptides essential for Vibrio tolerance in white leg shrimp, Penaeus vannamei, was investigated in this study, which included the determination of the safety levels of the extract. Tolerance of shrimp against Vibrio parahaemolyticus, a pathogenic bacteria that causes Acute Hepatopancreas Necrosis Disease (AHPND), was assessed on the basis of median lethal dose challenge survival (LD50=106cells/ml). Mortality was not observed 24h after exposure of 0.5-6g/L of the fruit extract, indicating that P. tectorius was not toxic to shrimp at these concentrations. A 24-h incubation of 2-6g/L of the fruit extract increased shrimp tolerance to V. parahaemolyticus, with survival doubled when the maximum dose tested in this study was used. Concomitant with a rise in survival was the increase in immune-related proteins, with Hsp70, ProPO, peroxinectin, penaeidin, crustin and transglutaminase increased 10, 11, 11, 0.4, 8 and 13-fold respectively. Histological examination of the hepatopancreas and muscle tissues of Vibrio-infected shrimp primed with P. tectorius extract revealed reduced signs of histopathological degeneration, possibly due to the accumulation of Hsp70, a molecular chaperone crucial to cellular protein folding, tissue repair and immune response of living organisms, including Penaeid shrimp.
Keywords
Penaeus vannamei, Pandanus tectorius, Vibrio, Immunity, Hsp70, HEAT-SHOCK-PROTEIN, INFECTIOUS MYONECROSIS VIRUS, ARTEMIA-FRANCISCANA-NAUPLII, FRESH-WATER PRAWN, LITOPENAEUS-VANNAMEI, TIGER PRAWN, MACROBRACHIUM-ROSENBERGII, PROPHENOLOXIDASE SYSTEM, ANTIMICROBIAL PEPTIDES, RECOMBINANT EXPRESSION

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MLA
Anirudhan, Anupa, et al. “Pandanus Tectorius Fruit Extract Promotes Hsp70 Accumulation, Immune-Related Genes Expression and Vibrio Parahaemolyticus Tolerance in the White-Leg Shrimp Penaeus Vannamei.” FISH & SHELLFISH IMMUNOLOGY, vol. 109, 2021, pp. 97–105, doi:10.1016/j.fsi.2020.12.011.
APA
Anirudhan, A., Okomoda, V. T., Mimi Iryani, M. T., Andriani, Y., Abd Wahid, M. E., Tan, M. P., … Sung, Y. Y. (2021). Pandanus tectorius fruit extract promotes Hsp70 accumulation, immune-related genes expression and Vibrio parahaemolyticus tolerance in the white-leg shrimp Penaeus vannamei. FISH & SHELLFISH IMMUNOLOGY, 109, 97–105. https://doi.org/10.1016/j.fsi.2020.12.011
Chicago author-date
Anirudhan, Anupa, Victor Tosin Okomoda, Mat Taib Mimi Iryani, Yosie Andriani, Mohd Effendy Abd Wahid, Min Pau Tan, Muhd Danish-Daniel, et al. 2021. “Pandanus Tectorius Fruit Extract Promotes Hsp70 Accumulation, Immune-Related Genes Expression and Vibrio Parahaemolyticus Tolerance in the White-Leg Shrimp Penaeus Vannamei.” FISH & SHELLFISH IMMUNOLOGY 109: 97–105. https://doi.org/10.1016/j.fsi.2020.12.011.
Chicago author-date (all authors)
Anirudhan, Anupa, Victor Tosin Okomoda, Mat Taib Mimi Iryani, Yosie Andriani, Mohd Effendy Abd Wahid, Min Pau Tan, Muhd Danish-Daniel, Li Lian Wong, Tengku Sifzizul Tengku-Muhammad, Wen Jye Mok, Patrick Sorgeloos, and Yeong Yik Sung. 2021. “Pandanus Tectorius Fruit Extract Promotes Hsp70 Accumulation, Immune-Related Genes Expression and Vibrio Parahaemolyticus Tolerance in the White-Leg Shrimp Penaeus Vannamei.” FISH & SHELLFISH IMMUNOLOGY 109: 97–105. doi:10.1016/j.fsi.2020.12.011.
Vancouver
1.
Anirudhan A, Okomoda VT, Mimi Iryani MT, Andriani Y, Abd Wahid ME, Tan MP, et al. Pandanus tectorius fruit extract promotes Hsp70 accumulation, immune-related genes expression and Vibrio parahaemolyticus tolerance in the white-leg shrimp Penaeus vannamei. FISH & SHELLFISH IMMUNOLOGY. 2021;109:97–105.
IEEE
[1]
A. Anirudhan et al., “Pandanus tectorius fruit extract promotes Hsp70 accumulation, immune-related genes expression and Vibrio parahaemolyticus tolerance in the white-leg shrimp Penaeus vannamei,” FISH & SHELLFISH IMMUNOLOGY, vol. 109, pp. 97–105, 2021.
@article{8697894,
  abstract     = {{Plants and herbal extracts are indispensable for controlling the spread of disease-causing bacteria, including those that infect aquatic organisms used in aquaculture. The use of plant or herbal extract is expected to be safe for aquatic animals and less harmful to the environment, as opposed to conventional therapeutic alternatives such as antibiotics that promote the occurrence of potential antibiotic-resistant bacteria when used improperly. The efficacy of Pandanus tectorius fruit extract in the regulation of Hsp70 expression, pro-phenoloxidase (ProPO), peroxinectin, penaeidin, crustin and transglutaminase, all immune peptides essential for Vibrio tolerance in white leg shrimp, Penaeus vannamei, was investigated in this study, which included the determination of the safety levels of the extract. Tolerance of shrimp against Vibrio parahaemolyticus, a pathogenic bacteria that causes Acute Hepatopancreas Necrosis Disease (AHPND), was assessed on the basis of median lethal dose challenge survival (LD50=106cells/ml). Mortality was not observed 24h after exposure of 0.5-6g/L of the fruit extract, indicating that P. tectorius was not toxic to shrimp at these concentrations. A 24-h incubation of 2-6g/L of the fruit extract increased shrimp tolerance to V. parahaemolyticus, with survival doubled when the maximum dose tested in this study was used. Concomitant with a rise in survival was the increase in immune-related proteins, with Hsp70, ProPO, peroxinectin, penaeidin, crustin and transglutaminase increased 10, 11, 11, 0.4, 8 and 13-fold respectively. Histological examination of the hepatopancreas and muscle tissues of Vibrio-infected shrimp primed with P. tectorius extract revealed reduced signs of histopathological degeneration, possibly due to the accumulation of Hsp70, a molecular chaperone crucial to cellular protein folding, tissue repair and immune response of living organisms, including Penaeid shrimp.}},
  author       = {{Anirudhan, Anupa and Okomoda, Victor Tosin and Mimi Iryani, Mat Taib and Andriani, Yosie and Abd Wahid, Mohd Effendy and Tan, Min Pau and Danish-Daniel, Muhd and Wong, Li Lian and Tengku-Muhammad, Tengku Sifzizul and Mok, Wen Jye and Sorgeloos, Patrick and Sung, Yeong Yik}},
  issn         = {{1050-4648}},
  journal      = {{FISH & SHELLFISH IMMUNOLOGY}},
  keywords     = {{Penaeus vannamei,Pandanus tectorius,Vibrio,Immunity,Hsp70,HEAT-SHOCK-PROTEIN,INFECTIOUS MYONECROSIS VIRUS,ARTEMIA-FRANCISCANA-NAUPLII,FRESH-WATER PRAWN,LITOPENAEUS-VANNAMEI,TIGER PRAWN,MACROBRACHIUM-ROSENBERGII,PROPHENOLOXIDASE SYSTEM,ANTIMICROBIAL PEPTIDES,RECOMBINANT EXPRESSION}},
  language     = {{eng}},
  pages        = {{97--105}},
  title        = {{Pandanus tectorius fruit extract promotes Hsp70 accumulation, immune-related genes expression and Vibrio parahaemolyticus tolerance in the white-leg shrimp Penaeus vannamei}},
  url          = {{http://doi.org/10.1016/j.fsi.2020.12.011}},
  volume       = {{109}},
  year         = {{2021}},
}
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