Advanced search
1 file | 994.22 KB Add to list

First international external quality assessment scheme of nucleic acid amplification tests for the detection of Schistosoma and soil-transmitted helminths, including Strongyloides : a pilot study

Author
Organization
Abstract
Background Nucleic acid amplification tests (NAATs) are increasingly being used as diagnostic tools for soil-transmitted helminths (STHs;Ascaris lumbricoides,Trichuris trichiura,Necator americanus,Ancylostoma duodenaleandA.ceylanicum),Strongyloides stercoralisandSchistosomain human stool. Currently, there is a large diversity of NAATs being applied, but an external quality assessment scheme (EQAS) for these diagnostics is lacking. An EQAS involves a blinded process where test results reported by a laboratory are compared to those reported by reference or expert laboratories, allowing for an objective assessment of the diagnostic performance of a laboratory. In the current study, we piloted an international EQAS for these helminths (i) to investigate the feasibility of designing and delivering an EQAS; (ii) to assess the diagnostic performance of laboratories; and (iii) to gain insights into the different NAAT protocols used. Methods and principal findings A panel of twelve stool samples and eight DNA samples was validated by six expert laboratories for the presence of six helminths (Ascaris,Trichuris,N.americanus,Ancylostoma,StrongyloidesandSchistosoma). Subsequently this panel was sent to 15 globally dispersed laboratories. We found a high degree of diversity among the different DNA extraction and NAAT protocols. Although most laboratories performed well, we could clearly identify the laboratories that were poorly performing. Conclusions/Significance We showed the technical feasibility of an international EQAS for the NAAT of STHs,StrongyloidesandSchistosoma. In addition, we documented that there are clear benefits for participating laboratories, as they can confirm and/or improve the diagnostic performance of their NAATs. Further research should aim to identify factors that explain poor performance of NAATs. Author summary Tests that detect parasite DNA in human stool are increasingly being used for the diagnosis of infections with intestinal worms, including schistosomiasis. To ensure the quality in diagnostic testing of these parasitic worms, it is important that laboratories evaluate the diagnostic performance of their DNA-based tests. This can best be achieved by participating in an external quality assessment scheme (EQAS). An EQAS involves a blinded process where test results reported by a laboratory are compared to those reported by reference or expert laboratories, allowing for an objective assessment of the diagnostic performance of a laboratory. Currently, such an EQAS for parasitic worms is lacking. We therefore piloted an international EQAS for the diagnosis of parasitic worms involving 15 laboratories in Africa, Asia, Australia, Europe, and North America. Although most laboratories performed well, we could clearly identify those laboratories that may need to improve their test protocol. We found that laboratories were using many different test protocols, and further research should aim to verify whether this has an impact on the performance of the diagnostic outcomes.
Keywords
DIAGNOSIS, MIGRANTS, DISEASES, BURDEN, PCR

Downloads

  • pntd.0008231.pdf
    • full text (Published version)
    • |
    • open access
    • |
    • PDF
    • |
    • 994.22 KB

Citation

Please use this url to cite or link to this publication:

MLA
Cools, Piet, et al. “First International External Quality Assessment Scheme of Nucleic Acid Amplification Tests for the Detection of Schistosoma and Soil-Transmitted Helminths, Including Strongyloides : A Pilot Study.” PLOS NEGLECTED TROPICAL DISEASES, vol. 14, no. 6, 2020, doi:10.1371/journal.pntd.0008231.
APA
Cools, P., van Lieshout, L., Koelewijn, R., Addiss, D., Ajjampur, S. S. R., Heda, M. A., … Levecke, B. (2020). First international external quality assessment scheme of nucleic acid amplification tests for the detection of Schistosoma and soil-transmitted helminths, including Strongyloides : a pilot study. PLOS NEGLECTED TROPICAL DISEASES, 14(6). https://doi.org/10.1371/journal.pntd.0008231
Chicago author-date
Cools, Piet, Lisette van Lieshout, Rob Koelewijn, David Addiss, Sitara S. R. Ajjampur, Mio Ayana Heda, Richard S. Bradbury, et al. 2020. “First International External Quality Assessment Scheme of Nucleic Acid Amplification Tests for the Detection of Schistosoma and Soil-Transmitted Helminths, Including Strongyloides : A Pilot Study.” PLOS NEGLECTED TROPICAL DISEASES 14 (6). https://doi.org/10.1371/journal.pntd.0008231.
Chicago author-date (all authors)
Cools, Piet, Lisette van Lieshout, Rob Koelewijn, David Addiss, Sitara S. R. Ajjampur, Mio Ayana Heda, Richard S. Bradbury, Jason L. Cantera, Daniel Dana Medebo, Kerstin Fischer, Rubina Imtiaz, Joyce Kabagenyi, James Lok, James McCarthy, Rojelio Mejia, Zeleke Mekonnen Kurmane, Sammy M. Njenga, Nurulhasanah Othman, Hongguang Shao, Rebecca Traub, Marjan Van Esbroeck, Jozef Vercruysse, Johnny Vlaminck, Steven A. Williams, Jaco J. Verweij, Jaap J. van Hellemond, and Bruno Levecke. 2020. “First International External Quality Assessment Scheme of Nucleic Acid Amplification Tests for the Detection of Schistosoma and Soil-Transmitted Helminths, Including Strongyloides : A Pilot Study.” PLOS NEGLECTED TROPICAL DISEASES 14 (6). doi:10.1371/journal.pntd.0008231.
Vancouver
1.
Cools P, van Lieshout L, Koelewijn R, Addiss D, Ajjampur SSR, Heda MA, et al. First international external quality assessment scheme of nucleic acid amplification tests for the detection of Schistosoma and soil-transmitted helminths, including Strongyloides : a pilot study. PLOS NEGLECTED TROPICAL DISEASES. 2020;14(6).
IEEE
[1]
P. Cools et al., “First international external quality assessment scheme of nucleic acid amplification tests for the detection of Schistosoma and soil-transmitted helminths, including Strongyloides : a pilot study,” PLOS NEGLECTED TROPICAL DISEASES, vol. 14, no. 6, 2020.
@article{8697838,
  abstract     = {Background Nucleic acid amplification tests (NAATs) are increasingly being used as diagnostic tools for soil-transmitted helminths (STHs;Ascaris lumbricoides,Trichuris trichiura,Necator americanus,Ancylostoma duodenaleandA.ceylanicum),Strongyloides stercoralisandSchistosomain human stool. Currently, there is a large diversity of NAATs being applied, but an external quality assessment scheme (EQAS) for these diagnostics is lacking. An EQAS involves a blinded process where test results reported by a laboratory are compared to those reported by reference or expert laboratories, allowing for an objective assessment of the diagnostic performance of a laboratory. In the current study, we piloted an international EQAS for these helminths (i) to investigate the feasibility of designing and delivering an EQAS; (ii) to assess the diagnostic performance of laboratories; and (iii) to gain insights into the different NAAT protocols used. Methods and principal findings A panel of twelve stool samples and eight DNA samples was validated by six expert laboratories for the presence of six helminths (Ascaris,Trichuris,N.americanus,Ancylostoma,StrongyloidesandSchistosoma). Subsequently this panel was sent to 15 globally dispersed laboratories. We found a high degree of diversity among the different DNA extraction and NAAT protocols. Although most laboratories performed well, we could clearly identify the laboratories that were poorly performing. Conclusions/Significance We showed the technical feasibility of an international EQAS for the NAAT of STHs,StrongyloidesandSchistosoma. In addition, we documented that there are clear benefits for participating laboratories, as they can confirm and/or improve the diagnostic performance of their NAATs. Further research should aim to identify factors that explain poor performance of NAATs. Author summary Tests that detect parasite DNA in human stool are increasingly being used for the diagnosis of infections with intestinal worms, including schistosomiasis. To ensure the quality in diagnostic testing of these parasitic worms, it is important that laboratories evaluate the diagnostic performance of their DNA-based tests. This can best be achieved by participating in an external quality assessment scheme (EQAS). An EQAS involves a blinded process where test results reported by a laboratory are compared to those reported by reference or expert laboratories, allowing for an objective assessment of the diagnostic performance of a laboratory. Currently, such an EQAS for parasitic worms is lacking. We therefore piloted an international EQAS for the diagnosis of parasitic worms involving 15 laboratories in Africa, Asia, Australia, Europe, and North America. Although most laboratories performed well, we could clearly identify those laboratories that may need to improve their test protocol. We found that laboratories were using many different test protocols, and further research should aim to verify whether this has an impact on the performance of the diagnostic outcomes.},
  articleno    = {e0008231},
  author       = {Cools, Piet and van Lieshout, Lisette and Koelewijn, Rob and Addiss, David and Ajjampur, Sitara S. R. and Heda, Mio Ayana and Bradbury, Richard S. and Cantera, Jason L. and Medebo, Daniel Dana and Fischer, Kerstin and Imtiaz, Rubina and Kabagenyi, Joyce and Lok, James and McCarthy, James and Mejia, Rojelio and Mekonnen Kurmane, Zeleke and Njenga, Sammy M. and Othman, Nurulhasanah and Shao, Hongguang and Traub, Rebecca and Van Esbroeck, Marjan and Vercruysse, Jozef and Vlaminck, Johnny and Williams, Steven A. and Verweij, Jaco J. and van Hellemond, Jaap J. and Levecke, Bruno},
  issn         = {1935-2735},
  journal      = {PLOS NEGLECTED TROPICAL DISEASES},
  keywords     = {DIAGNOSIS,MIGRANTS,DISEASES,BURDEN,PCR},
  language     = {eng},
  number       = {6},
  pages        = {19},
  title        = {First international external quality assessment scheme of nucleic acid amplification tests for the detection of Schistosoma and soil-transmitted helminths, including Strongyloides : a pilot study},
  url          = {http://dx.doi.org/10.1371/journal.pntd.0008231},
  volume       = {14},
  year         = {2020},
}

Altmetric
View in Altmetric
Web of Science
Times cited: