Advanced search
1 file | 1.25 MB Add to list

LC-MS compatible antiadsorption diluent for peptide analysis

Frederick Verbeke (UGent) , Nathalie Bracke (UGent) , Nathan Debunne (UGent) , Evelien Wynendaele (UGent) and Bart De Spiegeleer (UGent)
(2020) ANALYTICAL CHEMISTRY. 92(2). p.1712-1719
Author
Organization
Abstract
Analytical method development for peptides often proves challenging since these molecules can adsorb to the plastic or glass consumables used in the analysis. This adsorption causes considerable loss and unreliable results, especially in the lower concentration range. Therefore, a variety of antiadsorption strategies have previously been developed to cope with this adsorption, often however incompatible with direct liquid chromatography-mass spectrometry (LC MS) analysis. Here, a novel antiadsorption diluent is introduced, based on controlled hydrolysis and precipitation of bovine serum albumin. This diluent considerably decreases the adsorption of certain peptides to glass. Moreover, it is LC MS compatible and can also be used in combination with formic acid and/or acetonitrile addition.
Keywords
Analytical Chemistry, MASS-SPECTROMETRY, HUMAN PLASMA, PROTEIN, REPRODUCIBILITY, ADSORPTION, LOSSES

Downloads

  • (...).pdf
    • full text (Published version)
    • |
    • UGent only
    • |
    • PDF
    • |
    • 1.25 MB

Citation

Please use this url to cite or link to this publication:

MLA
Verbeke, Frederick, et al. “LC-MS Compatible Antiadsorption Diluent for Peptide Analysis.” ANALYTICAL CHEMISTRY, vol. 92, no. 2, 2020, pp. 1712–19, doi:10.1021/acs.analchem.9b01840.
APA
Verbeke, F., Bracke, N., Debunne, N., Wynendaele, E., & De Spiegeleer, B. (2020). LC-MS compatible antiadsorption diluent for peptide analysis. ANALYTICAL CHEMISTRY, 92(2), 1712–1719. https://doi.org/10.1021/acs.analchem.9b01840
Chicago author-date
Verbeke, Frederick, Nathalie Bracke, Nathan Debunne, Evelien Wynendaele, and Bart De Spiegeleer. 2020. “LC-MS Compatible Antiadsorption Diluent for Peptide Analysis.” ANALYTICAL CHEMISTRY 92 (2): 1712–19. https://doi.org/10.1021/acs.analchem.9b01840.
Chicago author-date (all authors)
Verbeke, Frederick, Nathalie Bracke, Nathan Debunne, Evelien Wynendaele, and Bart De Spiegeleer. 2020. “LC-MS Compatible Antiadsorption Diluent for Peptide Analysis.” ANALYTICAL CHEMISTRY 92 (2): 1712–1719. doi:10.1021/acs.analchem.9b01840.
Vancouver
1.
Verbeke F, Bracke N, Debunne N, Wynendaele E, De Spiegeleer B. LC-MS compatible antiadsorption diluent for peptide analysis. ANALYTICAL CHEMISTRY. 2020;92(2):1712–9.
IEEE
[1]
F. Verbeke, N. Bracke, N. Debunne, E. Wynendaele, and B. De Spiegeleer, “LC-MS compatible antiadsorption diluent for peptide analysis,” ANALYTICAL CHEMISTRY, vol. 92, no. 2, pp. 1712–1719, 2020.
@article{8697445,
  abstract     = {{Analytical method development for peptides often proves challenging since these molecules can adsorb to the plastic or glass consumables used in the analysis. This adsorption causes considerable loss and unreliable results, especially in the lower concentration range. Therefore, a variety of antiadsorption strategies have previously been developed to cope with this adsorption, often however incompatible with direct liquid chromatography-mass spectrometry (LC MS) analysis. Here, a novel antiadsorption diluent is introduced, based on controlled hydrolysis and precipitation of bovine serum albumin. This diluent considerably decreases the adsorption of certain peptides to glass. Moreover, it is LC MS compatible and can also be used in combination with formic acid and/or acetonitrile addition.}},
  author       = {{Verbeke, Frederick and Bracke, Nathalie and Debunne, Nathan and Wynendaele, Evelien and De Spiegeleer, Bart}},
  issn         = {{0003-2700}},
  journal      = {{ANALYTICAL CHEMISTRY}},
  keywords     = {{Analytical Chemistry,MASS-SPECTROMETRY,HUMAN PLASMA,PROTEIN,REPRODUCIBILITY,ADSORPTION,LOSSES}},
  language     = {{eng}},
  number       = {{2}},
  pages        = {{1712--1719}},
  title        = {{LC-MS compatible antiadsorption diluent for peptide analysis}},
  url          = {{http://doi.org/10.1021/acs.analchem.9b01840}},
  volume       = {{92}},
  year         = {{2020}},
}

Altmetric
View in Altmetric
Web of Science
Times cited: