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Comparative study of preimplantation development following distinct assisted oocyte activation protocols in a PLC-zeta knockout mouse model

(2020) MOLECULAR HUMAN REPRODUCTION. 26(11). p.801-815
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Abstract
Mammalian fertilization encompasses a series of Ca2+ oscillations initiated by the sperm factor phospholipase C zeta (PLC zeta). Some studies have shown that altering the Ca2+ oscillatory regime at fertilization affects preimplantation blastocyst development. However, assisted oocyte activation (AOA) protocols can induce oocyte activation in a manner that diverges profoundly from the physiological Ca2+ profiling. In our study, we used the newly developed PLC-null sperm to investigate the independent effect of AOA on mouse preimplantation embryogenesis. Based on previous findings, we hypothesized that AOA protocols with Ca2+ oscillatory responses might improve blastocyst formation rates and differing Ca2+ profiles might alter blastocyst transcriptomes. A total of 326 MII B6D2F1-oocytes were used to describe Ca2+ profiles and to compare embryonic development and individual blastocyst transcriptomes between four control conditions: C I (in-vivo fertilization), C2 (ICSI control sperm), C3 (parthenogenesis) and C4 (ICSI-PLC zeta-KO sperm) and four AOA groups: AOA1 (human recombinant PLC zeta, AOA2 (Sr2+), AOA3 (ionomycin) and AOA4 (TPEN). All groups revealed remarkable variations in their Ca2+ profiles; however, oocyte activation rates were comparable between the controls (91.1% +/- 13.8%) and AOA (86.9% +/- 11.1%) groups. AOA methods which enable Ca2+ oscillatory responses (AOA1: 41% and AOA2: 75%) or single Ca2+ transients (AOA3: 50%) showed no significantly different blastocyst rates compared to ICSI control group (C2: 70%). In contrast, we observed a significant decrease in compaction (53% vs. 83%) and blastocyst rates (41% vs. 70%) in the absence of an initial Ca2+ trigger (AOA4) compared with the C2 group. Transcription profiles did not identify significant differences in gene expression levels between the ICSI control group (C2) and the four AOA groups.
Keywords
assisted oocyte activation, calcium oscillations, blastocyst development, phospholipase C zeta, transcriptomics, animal model, PHOSPHOLIPASE-C-ZETA, EGG-ACTIVATION, CA2+ OSCILLATIONS, PARTHENOGENETIC ACTIVATION, FERTILIZATION FAILURE, CALCIUM OSCILLATIONS, ICSI, SIGNAL, INFERTILITY, INJECTION

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MLA
Ferrer Buitrago, Minerva, et al. “Comparative Study of Preimplantation Development Following Distinct Assisted Oocyte Activation Protocols in a PLC-Zeta Knockout Mouse Model.” MOLECULAR HUMAN REPRODUCTION, vol. 26, no. 11, 2020, pp. 801–15, doi:10.1093/molehr/gaaa060.
APA
Ferrer Buitrago, M., Tilleman, L., Thys, V., Hachem, A., Boel, A., Van Nieuwerburgh, F., … Heindryckx, B. (2020). Comparative study of preimplantation development following distinct assisted oocyte activation protocols in a PLC-zeta knockout mouse model. MOLECULAR HUMAN REPRODUCTION, 26(11), 801–815. https://doi.org/10.1093/molehr/gaaa060
Chicago author-date
Ferrer Buitrago, Minerva, Laurentijn Tilleman, Vanessa Thys, A Hachem, Annekatrien Boel, Filip Van Nieuwerburgh, Dieter Deforce, et al. 2020. “Comparative Study of Preimplantation Development Following Distinct Assisted Oocyte Activation Protocols in a PLC-Zeta Knockout Mouse Model.” MOLECULAR HUMAN REPRODUCTION 26 (11): 801–15. https://doi.org/10.1093/molehr/gaaa060.
Chicago author-date (all authors)
Ferrer Buitrago, Minerva, Laurentijn Tilleman, Vanessa Thys, A Hachem, Annekatrien Boel, Filip Van Nieuwerburgh, Dieter Deforce, Luc Leybaert, Petra De Sutter, J. Parrington, and Björn Heindryckx. 2020. “Comparative Study of Preimplantation Development Following Distinct Assisted Oocyte Activation Protocols in a PLC-Zeta Knockout Mouse Model.” MOLECULAR HUMAN REPRODUCTION 26 (11): 801–815. doi:10.1093/molehr/gaaa060.
Vancouver
1.
Ferrer Buitrago M, Tilleman L, Thys V, Hachem A, Boel A, Van Nieuwerburgh F, et al. Comparative study of preimplantation development following distinct assisted oocyte activation protocols in a PLC-zeta knockout mouse model. MOLECULAR HUMAN REPRODUCTION. 2020;26(11):801–15.
IEEE
[1]
M. Ferrer Buitrago et al., “Comparative study of preimplantation development following distinct assisted oocyte activation protocols in a PLC-zeta knockout mouse model,” MOLECULAR HUMAN REPRODUCTION, vol. 26, no. 11, pp. 801–815, 2020.
@article{8697128,
  abstract     = {{Mammalian fertilization encompasses a series of Ca2+ oscillations initiated by the sperm factor phospholipase C zeta (PLC zeta). Some studies have shown that altering the Ca2+ oscillatory regime at fertilization affects preimplantation blastocyst development. However, assisted oocyte activation (AOA) protocols can induce oocyte activation in a manner that diverges profoundly from the physiological Ca2+ profiling. In our study, we used the newly developed PLC-null sperm to investigate the independent effect of AOA on mouse preimplantation embryogenesis. Based on previous findings, we hypothesized that AOA protocols with Ca2+ oscillatory responses might improve blastocyst formation rates and differing Ca2+ profiles might alter blastocyst transcriptomes. A total of 326 MII B6D2F1-oocytes were used to describe Ca2+ profiles and to compare embryonic development and individual blastocyst transcriptomes between four control conditions: C I (in-vivo fertilization), C2 (ICSI control sperm), C3 (parthenogenesis) and C4 (ICSI-PLC zeta-KO sperm) and four AOA groups: AOA1 (human recombinant PLC zeta, AOA2 (Sr2+), AOA3 (ionomycin) and AOA4 (TPEN). All groups revealed remarkable variations in their Ca2+ profiles; however, oocyte activation rates were comparable between the controls (91.1% +/- 13.8%) and AOA (86.9% +/- 11.1%) groups. AOA methods which enable Ca2+ oscillatory responses (AOA1: 41% and AOA2: 75%) or single Ca2+ transients (AOA3: 50%) showed no significantly different blastocyst rates compared to ICSI control group (C2: 70%). In contrast, we observed a significant decrease in compaction (53% vs. 83%) and blastocyst rates (41% vs. 70%) in the absence of an initial Ca2+ trigger (AOA4) compared with the C2 group. Transcription profiles did not identify significant differences in gene expression levels between the ICSI control group (C2) and the four AOA groups.}},
  author       = {{Ferrer Buitrago, Minerva and Tilleman, Laurentijn and Thys, Vanessa and Hachem, A and Boel, Annekatrien and Van Nieuwerburgh, Filip and Deforce, Dieter and Leybaert, Luc and De Sutter, Petra and Parrington, J. and Heindryckx, Björn}},
  issn         = {{1460-2407}},
  journal      = {{MOLECULAR HUMAN REPRODUCTION}},
  keywords     = {{assisted oocyte activation,calcium oscillations,blastocyst development,phospholipase C zeta,transcriptomics,animal model,PHOSPHOLIPASE-C-ZETA,EGG-ACTIVATION,CA2+ OSCILLATIONS,PARTHENOGENETIC ACTIVATION,FERTILIZATION FAILURE,CALCIUM OSCILLATIONS,ICSI,SIGNAL,INFERTILITY,INJECTION}},
  language     = {{eng}},
  number       = {{11}},
  pages        = {{801--815}},
  title        = {{Comparative study of preimplantation development following distinct assisted oocyte activation protocols in a PLC-zeta knockout mouse model}},
  url          = {{http://dx.doi.org/10.1093/molehr/gaaa060}},
  volume       = {{26}},
  year         = {{2020}},
}

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