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β-glucan-induced IL-10 secretion by monocytes triggers porcine NK cell cytotoxicity

Leen Hermans (UGent) , Steffi De Pelsmaeker, Sofie Denaeghel (UGent) , Eric Cox (UGent) , Herman Favoreel (UGent) and Bert Devriendt (UGent)
(2021) FRONTIERS IN IMMUNOLOGY. 12.
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Abstract
Beta-glucans are naturally occurring polysaccharides present in cell walls of fungi, yeast, bacteria, cereals, seaweed, and algae. These microbe-associated molecular patterns (MAMPs) possess immunomodulatory properties. In human, it has been suggested that NK cells can be activated by β-glucans. Here, we aimed to elucidate whether β-glucans modulate porcine NK cell responses <jats:italic>in vitro</jats:italic> and if so, how these effects are mediated. We investigated the effect of two β-glucans, Macrogard and Curdlan, which differ in solubility and structure. Direct addition of β-glucans to purified porcine NK cells did not affect cytotoxicity of these cells against K562 target cells. However, when using PBMC instead of purified NK cells, β-glucan addition significantly increased NK cell-mediated cytotoxicity. This effect depended on factors secreted by CD14+ monocytes upon β-glucan priming. Further analysis showed that monocytes secrete TNF-α, IL-6, and IL-10 upon β-glucan addition. Of these, IL-10 turned out to play a critical role in β-glucan-triggered NK cell cytotoxicity, since depletion of IL-10 completely abrogated the β-glucan-induced increase in cytotoxicity. Furthermore, addition of recombinant IL-10 to purified NK cells was sufficient to enhance cytotoxicity. In conclusion, we show that β-glucans trigger IL-10 secretion by porcine monocytes, which in turn leads to increased NK cell cytotoxicity, and thereby identify IL-10 as a potent stimulus of porcine NK cell cytotoxicity.
Keywords
NK cells, IL-10, &#946, -glucans, macrogard, curdlan, immune modulation, monocyte

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Citation

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MLA
Hermans, Leen, et al. “β-Glucan-Induced IL-10 Secretion by Monocytes Triggers Porcine NK Cell Cytotoxicity.” FRONTIERS IN IMMUNOLOGY, vol. 12, 2021, doi:10.3389/fimmu.2021.634402.
APA
Hermans, L., De Pelsmaeker, S., Denaeghel, S., Cox, E., Favoreel, H., & Devriendt, B. (2021). β-glucan-induced IL-10 secretion by monocytes triggers porcine NK cell cytotoxicity. FRONTIERS IN IMMUNOLOGY, 12. https://doi.org/10.3389/fimmu.2021.634402
Chicago author-date
Hermans, Leen, Steffi De Pelsmaeker, Sofie Denaeghel, Eric Cox, Herman Favoreel, and Bert Devriendt. 2021. “β-Glucan-Induced IL-10 Secretion by Monocytes Triggers Porcine NK Cell Cytotoxicity.” FRONTIERS IN IMMUNOLOGY 12. https://doi.org/10.3389/fimmu.2021.634402.
Chicago author-date (all authors)
Hermans, Leen, Steffi De Pelsmaeker, Sofie Denaeghel, Eric Cox, Herman Favoreel, and Bert Devriendt. 2021. “β-Glucan-Induced IL-10 Secretion by Monocytes Triggers Porcine NK Cell Cytotoxicity.” FRONTIERS IN IMMUNOLOGY 12. doi:10.3389/fimmu.2021.634402.
Vancouver
1.
Hermans L, De Pelsmaeker S, Denaeghel S, Cox E, Favoreel H, Devriendt B. β-glucan-induced IL-10 secretion by monocytes triggers porcine NK cell cytotoxicity. FRONTIERS IN IMMUNOLOGY. 2021;12.
IEEE
[1]
L. Hermans, S. De Pelsmaeker, S. Denaeghel, E. Cox, H. Favoreel, and B. Devriendt, “β-glucan-induced IL-10 secretion by monocytes triggers porcine NK cell cytotoxicity,” FRONTIERS IN IMMUNOLOGY, vol. 12, 2021.
@article{8696808,
  abstract     = {{Beta-glucans are naturally occurring polysaccharides present in cell walls of fungi, yeast, bacteria, cereals, seaweed, and algae. These microbe-associated molecular patterns (MAMPs) possess immunomodulatory properties. In human, it has been suggested that NK cells can be activated by β-glucans. Here, we aimed to elucidate whether β-glucans modulate porcine NK cell responses <jats:italic>in vitro</jats:italic> and if so, how these effects are mediated. We investigated the effect of two β-glucans, Macrogard and Curdlan, which differ in solubility and structure. Direct addition of β-glucans to purified porcine NK cells did not affect cytotoxicity of these cells against K562 target cells. However, when using PBMC instead of purified NK cells, β-glucan addition significantly increased NK cell-mediated cytotoxicity. This effect depended on factors secreted by CD14+ monocytes upon β-glucan priming. Further analysis showed that monocytes secrete TNF-α, IL-6, and IL-10 upon β-glucan addition. Of these, IL-10 turned out to play a critical role in β-glucan-triggered NK cell cytotoxicity, since depletion of IL-10 completely abrogated the β-glucan-induced increase in cytotoxicity. Furthermore, addition of recombinant IL-10 to purified NK cells was sufficient to enhance cytotoxicity. In conclusion, we show that β-glucans trigger IL-10 secretion by porcine monocytes, which in turn leads to increased NK cell cytotoxicity, and thereby identify IL-10 as a potent stimulus of porcine NK cell cytotoxicity.}},
  articleno    = {{634402}},
  author       = {{Hermans, Leen and De Pelsmaeker, Steffi and Denaeghel, Sofie and Cox, Eric and Favoreel, Herman and Devriendt, Bert}},
  issn         = {{1664-3224}},
  journal      = {{FRONTIERS IN IMMUNOLOGY}},
  keywords     = {{NK cells,IL-10,&#946,-glucans,macrogard,curdlan,immune modulation,monocyte}},
  language     = {{eng}},
  pages        = {{14}},
  title        = {{β-glucan-induced IL-10 secretion by monocytes triggers porcine NK cell cytotoxicity}},
  url          = {{http://dx.doi.org/10.3389/fimmu.2021.634402}},
  volume       = {{12}},
  year         = {{2021}},
}
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