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Molecular architecture of the endocytic TPLATE complex

Klaas Yperman (UGent) , Jie Wang (UGent) , Dominique Eeckhout (UGent) , Joanna Winkler (UGent) , Lam Dai Vu (UGent) , Michaël Vandorpe (UGent) , Peter Grones (UGent) , Evelien Mylle (UGent) , Michael Kraus (UGent) , Romain Merceron (UGent) , et al.
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Abstract
Eukaryotic cells rely on endocytosis to regulate their plasma membrane proteome and lipidome. Most eukaryotic groups, except fungi and animals, have retained the evolutionary ancient TSET complex as an endocytic regulator. Unlike other coatomer complexes, structural insight into TSET is lacking. Here, we reveal the molecular architecture of plant TSET [TPLATE complex (TPC)] using an integrative structural approach. We identify crucial roles for specific TSET subunits in complex assembly and membrane interaction. Our data therefore generate fresh insight into the differences between the hexameric TSET in Dictyostelium and the octameric TPC in plants. Structural elucidation of this ancient adaptor complex represents the missing piece in the coatomer puzzle and vastly advances our functional as well as evolutionary insight into the process of endocytosis.

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MLA
Yperman, Klaas, et al. “Molecular Architecture of the Endocytic TPLATE Complex.” SCIENCE ADVANCES, vol. 7, no. 9, 2021, doi:10.1126/sciadv.abe7999.
APA
Yperman, K., Wang, J., Eeckhout, D., Winkler, J., Vu, L. D., Vandorpe, M., … Pleskot, R. (2021). Molecular architecture of the endocytic TPLATE complex. SCIENCE ADVANCES, 7(9). https://doi.org/10.1126/sciadv.abe7999
Chicago author-date
Yperman, Klaas, Jie Wang, Dominique Eeckhout, Joanna Winkler, Lam Dai Vu, Michaël Vandorpe, Peter Grones, et al. 2021. “Molecular Architecture of the Endocytic TPLATE Complex.” SCIENCE ADVANCES 7 (9). https://doi.org/10.1126/sciadv.abe7999.
Chicago author-date (all authors)
Yperman, Klaas, Jie Wang, Dominique Eeckhout, Joanna Winkler, Lam Dai Vu, Michaël Vandorpe, Peter Grones, Evelien Mylle, Michael Kraus, Romain Merceron, Jonah Nolf, Eliana Mor, Pieter De Bruyn, Remy Loris, Martin Potocký, Savvas Savvides, Bert De Rybel, Geert De Jaeger, Daniël Van Damme, and Roman Pleskot. 2021. “Molecular Architecture of the Endocytic TPLATE Complex.” SCIENCE ADVANCES 7 (9). doi:10.1126/sciadv.abe7999.
Vancouver
1.
Yperman K, Wang J, Eeckhout D, Winkler J, Vu LD, Vandorpe M, et al. Molecular architecture of the endocytic TPLATE complex. SCIENCE ADVANCES. 2021;7(9).
IEEE
[1]
K. Yperman et al., “Molecular architecture of the endocytic TPLATE complex,” SCIENCE ADVANCES, vol. 7, no. 9, 2021.
@article{8696408,
  abstract     = {{Eukaryotic cells rely on endocytosis to regulate their plasma membrane proteome and lipidome. Most eukaryotic groups, except fungi and animals, have retained the evolutionary ancient TSET complex as an endocytic regulator. Unlike other coatomer complexes, structural insight into TSET is lacking. Here, we reveal the molecular architecture of plant TSET [TPLATE complex (TPC)] using an integrative structural approach. We identify crucial roles for specific TSET subunits in complex assembly and membrane interaction. Our data therefore generate fresh insight into the differences between the hexameric TSET in Dictyostelium and the octameric TPC in plants. Structural elucidation of this ancient adaptor complex represents the missing piece in the coatomer puzzle and vastly advances our functional as well as evolutionary insight into the process of endocytosis.}},
  articleno    = {{eabe7999}},
  author       = {{Yperman, Klaas and Wang, Jie and Eeckhout, Dominique and Winkler, Joanna and Vu, Lam Dai and Vandorpe, Michaël and Grones, Peter and Mylle, Evelien and Kraus, Michael and Merceron, Romain and Nolf, Jonah and Mor, Eliana and De Bruyn, Pieter and Loris, Remy and Potocký, Martin and Savvides, Savvas and De Rybel, Bert and De Jaeger, Geert and Van Damme, Daniël and Pleskot, Roman}},
  issn         = {{2375-2548}},
  journal      = {{SCIENCE ADVANCES}},
  language     = {{eng}},
  number       = {{9}},
  pages        = {{17}},
  title        = {{Molecular architecture of the endocytic TPLATE complex}},
  url          = {{http://dx.doi.org/10.1126/sciadv.abe7999}},
  volume       = {{7}},
  year         = {{2021}},
}

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