Advanced search
1 file | 3.55 MB Add to list
Author
Organization
Abstract
Honey bees have a sting which allows them to inject venomous substances into the body of an opponent or attacker. As the sting originates from a modified ovipositor, it only occurs in the female insect, and this is a defining feature of the bee species that belong to a subclade of the Hymenoptera called Aculeata. There is considerable interest in bee venom research, primarily because of an important subset of the human population who will develop a sometimes life threatening allergic response after a bee sting. However, the use of honey bee venom goes much further, with alleged healing properties in ancient therapies and recent research. The present paper aims to standardize selected methods for honey bee venom research. It covers different methods of venom collection, characterization and storage. Much attention was also addressed to the determination of the biological activity of the venom and its use in the context of biomedical research, more specifically venom allergy. Finally, the procedure for the assignment of new venom allergens has been presented. Las abejas meliferas tienen un aguijon que les permite inyectar sustancias venenosas en el cuerpo de un oponente o atacante. El aguijon es un ovipositor modificado que solo se manifiesta en el insecto hembra, siendo este una caracteristica que define a las especies de abejas que pertenecen al subclado de himenopteros llamada Aculeata. Hay un interes considerable en la investigacion del veneno de abeja, principalmente debido a que un porcentaje importante de la poblacion humana desarrollara una respuesta alergica - a veces mortal - a la picadura de abeja. Sin embargo, el uso del veneno de la abeja melifera abarca mucho mas, con presuntas propiedades curativas en terapias antiguas e investigaciones recientes. El presente trabajo tiene como objetivo estandarizar metodos seleccionados para la investigacion del veneno de las abejas meliferas. Cubre diferentes metodos de recoleccion, caracterizacion y almacenamiento de veneno. Tambien se presto mucha atencion a la determinacion de la actividad biologica del veneno y su uso en el contexto de la investigacion biomedica, mas especificamente la alergia al veneno. Finalmente, se ha presentado el procedimiento para la asignacion de nuevos alergenos de veneno.
Keywords
POLYACRYLAMIDE-GEL ELECTROPHORESIS, BEE VENOM, HYALURONIDASE ACTIVITY, ENZYMATIC-ACTIVITY, ACID-PHOSPHATASE, HONEY-BEES, HYMENOPTERA VENOM, MOLECULAR-CLONING, PHOSPHOLIPASE-A, FLOW-CYTOMETRY, acid phosphatase, Apis mellifera, esterase, honey bee, hyaluronidase, phospholipase A2, sting allergy, venom characterization, venom, collection, venom composition, venom storage, COLOSS, BEEBOOK

Downloads

  • Standard methods for Apis mellifera venom research.pdf
    • full text (Published version)
    • |
    • open access
    • |
    • PDF
    • |
    • 3.55 MB

Citation

Please use this url to cite or link to this publication:

MLA
de Graaf, Dirk, et al. “Standard Methods for Apis Mellifera Venom Research.” JOURNAL OF APICULTURAL RESEARCH, 2021, doi:10.1080/00218839.2020.1801073.
APA
de Graaf, D., Regina Brochetto Braga, M., Maria Magalhaes de Abreu, R., Blank, S., Bridts, C. H., De Clerck, L. S., … Van Vaerenbergh, M. (2021). Standard methods for Apis mellifera venom research. JOURNAL OF APICULTURAL RESEARCH. https://doi.org/10.1080/00218839.2020.1801073
Chicago author-date
Graaf, Dirk de, Marcia Regina Brochetto Braga, Rusleyd Maria Magalhaes de Abreu, Simon Blank, Chris H. Bridts, Luc S. De Clerck, Bart Devreese, et al. 2021. “Standard Methods for Apis Mellifera Venom Research.” JOURNAL OF APICULTURAL RESEARCH. https://doi.org/10.1080/00218839.2020.1801073.
Chicago author-date (all authors)
de Graaf, Dirk, Marcia Regina Brochetto Braga, Rusleyd Maria Magalhaes de Abreu, Simon Blank, Chris H. Bridts, Luc S. De Clerck, Bart Devreese, Didier G. Ebo, Timothy J. Ferris, Margo M. Hagendorens, Debora Lais Justo Jacomini, Iliya Kanchev, Zenon J. Kokot, Jan Matysiak, Christel Mertens, Vito Sabato, Athina L. Van Gasse, and Matthias Van Vaerenbergh. 2021. “Standard Methods for Apis Mellifera Venom Research.” JOURNAL OF APICULTURAL RESEARCH. doi:10.1080/00218839.2020.1801073.
Vancouver
1.
de Graaf D, Regina Brochetto Braga M, Maria Magalhaes de Abreu R, Blank S, Bridts CH, De Clerck LS, et al. Standard methods for Apis mellifera venom research. JOURNAL OF APICULTURAL RESEARCH. 2021;
IEEE
[1]
D. de Graaf et al., “Standard methods for Apis mellifera venom research,” JOURNAL OF APICULTURAL RESEARCH, 2021.
@article{8696349,
  abstract     = {{Honey bees have a sting which allows them to inject venomous substances into the body of an opponent or attacker. As the sting originates from a modified ovipositor, it only occurs in the female insect, and this is a defining feature of the bee species that belong to a subclade of the Hymenoptera called Aculeata. There is considerable interest in bee venom research, primarily because of an important subset of the human population who will develop a sometimes life threatening allergic response after a bee sting. However, the use of honey bee venom goes much further, with alleged healing properties in ancient therapies and recent research. The present paper aims to standardize selected methods for honey bee venom research. It covers different methods of venom collection, characterization and storage. Much attention was also addressed to the determination of the biological activity of the venom and its use in the context of biomedical research, more specifically venom allergy. Finally, the procedure for the assignment of new venom allergens has been presented. Las abejas meliferas tienen un aguijon que les permite inyectar sustancias venenosas en el cuerpo de un oponente o atacante. El aguijon es un ovipositor modificado que solo se manifiesta en el insecto hembra, siendo este una caracteristica que define a las especies de abejas que pertenecen al subclado de himenopteros llamada Aculeata. Hay un interes considerable en la investigacion del veneno de abeja, principalmente debido a que un porcentaje importante de la poblacion humana desarrollara una respuesta alergica - a veces mortal - a la picadura de abeja. Sin embargo, el uso del veneno de la abeja melifera abarca mucho mas, con presuntas propiedades curativas en terapias antiguas e investigaciones recientes. El presente trabajo tiene como objetivo estandarizar metodos seleccionados para la investigacion del veneno de las abejas meliferas. Cubre diferentes metodos de recoleccion, caracterizacion y almacenamiento de veneno. Tambien se presto mucha atencion a la determinacion de la actividad biologica del veneno y su uso en el contexto de la investigacion biomedica, mas especificamente la alergia al veneno. Finalmente, se ha presentado el procedimiento para la asignacion de nuevos alergenos de veneno.}},
  author       = {{de Graaf, Dirk and Regina Brochetto Braga, Marcia and Maria Magalhaes de Abreu, Rusleyd and Blank, Simon and Bridts, Chris H. and De Clerck, Luc S. and Devreese, Bart and Ebo, Didier G. and Ferris, Timothy J. and Hagendorens, Margo M. and Lais Justo Jacomini, Debora and Kanchev, Iliya and Kokot, Zenon J. and Matysiak, Jan and Mertens, Christel and Sabato, Vito and Van Gasse, Athina L. and Van Vaerenbergh, Matthias}},
  issn         = {{0021-8839}},
  journal      = {{JOURNAL OF APICULTURAL RESEARCH}},
  keywords     = {{POLYACRYLAMIDE-GEL ELECTROPHORESIS,BEE VENOM,HYALURONIDASE ACTIVITY,ENZYMATIC-ACTIVITY,ACID-PHOSPHATASE,HONEY-BEES,HYMENOPTERA VENOM,MOLECULAR-CLONING,PHOSPHOLIPASE-A,FLOW-CYTOMETRY,acid phosphatase,Apis mellifera,esterase,honey bee,hyaluronidase,phospholipase A2,sting allergy,venom characterization,venom,collection,venom composition,venom storage,COLOSS,BEEBOOK}},
  language     = {{eng}},
  pages        = {{31}},
  title        = {{Standard methods for Apis mellifera venom research}},
  url          = {{http://dx.doi.org/10.1080/00218839.2020.1801073}},
  year         = {{2021}},
}

Altmetric
View in Altmetric
Web of Science
Times cited: