Purification of GNA-related lectins from natural sources
(2020)
Lectin purification and analysis : methods and protocols.
In Methods in Molecular Biology
2132.
p.413-419
- Author
- Els Van Damme (UGent)
- Organization
- Abstract
- The Galanthus nivalis lectin, abbreviated as GNA, is the model protein for a large group of mannose-binding lectins. Here, we describe the purification of GNA starting from dry bulbs. Using a combination of ion exchange chromatography and affinity chromatography on mannose-Sepharose, a highly pure preparation of GNA can be obtained.
- Keywords
- Agglutination activity, Glycan binding, GNA, Plant lectin, Snowdrop
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-8696027
- MLA
- Van Damme, Els. “Purification of GNA-Related Lectins from Natural Sources.” Lectin Purification and Analysis : Methods and Protocols, vol. 2132, Humana, 2020, pp. 413–19, doi:10.1007/978-1-0716-0430-4_40.
- APA
- Van Damme, E. (2020). Purification of GNA-related lectins from natural sources. In Lectin purification and analysis : methods and protocols (Vol. 2132, pp. 413–419). New York, NY: Humana. https://doi.org/10.1007/978-1-0716-0430-4_40
- Chicago author-date
- Van Damme, Els. 2020. “Purification of GNA-Related Lectins from Natural Sources.” In Lectin Purification and Analysis : Methods and Protocols, 2132:413–19. New York, NY: Humana. https://doi.org/10.1007/978-1-0716-0430-4_40.
- Chicago author-date (all authors)
- Van Damme, Els. 2020. “Purification of GNA-Related Lectins from Natural Sources.” In Lectin Purification and Analysis : Methods and Protocols, 2132:413–419. New York, NY: Humana. doi:10.1007/978-1-0716-0430-4_40.
- Vancouver
- 1.Van Damme E. Purification of GNA-related lectins from natural sources. In: Lectin purification and analysis : methods and protocols. New York, NY: Humana; 2020. p. 413–9.
- IEEE
- [1]E. Van Damme, “Purification of GNA-related lectins from natural sources,” in Lectin purification and analysis : methods and protocols, vol. 2132, New York, NY: Humana, 2020, pp. 413–419.
@incollection{8696027,
abstract = {{The Galanthus nivalis lectin, abbreviated as GNA, is the model protein for a large group of mannose-binding lectins. Here, we describe the purification of GNA starting from dry bulbs. Using a combination of ion exchange chromatography and affinity chromatography on mannose-Sepharose, a highly pure preparation of GNA can be obtained.}},
author = {{Van Damme, Els}},
booktitle = {{Lectin purification and analysis : methods and protocols}},
isbn = {{9781071604298}},
issn = {{1064-3745}},
keywords = {{Agglutination activity,Glycan binding,GNA,Plant lectin,Snowdrop}},
language = {{eng}},
pages = {{413--419}},
publisher = {{Humana}},
series = {{Methods in Molecular Biology}},
title = {{Purification of GNA-related lectins from natural sources}},
url = {{http://dx.doi.org/10.1007/978-1-0716-0430-4_40}},
volume = {{2132}},
year = {{2020}},
}
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