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Purification of GNA-related lectins from natural sources

Els Van Damme (UGent)
Author
Organization
Abstract
The Galanthus nivalis lectin, abbreviated as GNA, is the model protein for a large group of mannose-binding lectins. Here, we describe the purification of GNA starting from dry bulbs. Using a combination of ion exchange chromatography and affinity chromatography on mannose-Sepharose, a highly pure preparation of GNA can be obtained.
Keywords
Agglutination activity, Glycan binding, GNA, Plant lectin, Snowdrop

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Citation

Please use this url to cite or link to this publication:

MLA
Van Damme, Els. “Purification of GNA-Related Lectins from Natural Sources.” Lectin Purification and Analysis : Methods and Protocols, vol. 2132, Humana, 2020, pp. 413–19, doi:10.1007/978-1-0716-0430-4_40.
APA
Van Damme, E. (2020). Purification of GNA-related lectins from natural sources. In Lectin purification and analysis : methods and protocols (Vol. 2132, pp. 413–419). New York, NY: Humana. https://doi.org/10.1007/978-1-0716-0430-4_40
Chicago author-date
Van Damme, Els. 2020. “Purification of GNA-Related Lectins from Natural Sources.” In Lectin Purification and Analysis : Methods and Protocols, 2132:413–19. New York, NY: Humana. https://doi.org/10.1007/978-1-0716-0430-4_40.
Chicago author-date (all authors)
Van Damme, Els. 2020. “Purification of GNA-Related Lectins from Natural Sources.” In Lectin Purification and Analysis : Methods and Protocols, 2132:413–419. New York, NY: Humana. doi:10.1007/978-1-0716-0430-4_40.
Vancouver
1.
Van Damme E. Purification of GNA-related lectins from natural sources. In: Lectin purification and analysis : methods and protocols. New York, NY: Humana; 2020. p. 413–9.
IEEE
[1]
E. Van Damme, “Purification of GNA-related lectins from natural sources,” in Lectin purification and analysis : methods and protocols, vol. 2132, New York, NY: Humana, 2020, pp. 413–419.
@incollection{8696027,
  abstract     = {{The Galanthus nivalis lectin, abbreviated as GNA, is the model protein for a large group of mannose-binding lectins. Here, we describe the purification of GNA starting from dry bulbs. Using a combination of ion exchange chromatography and affinity chromatography on mannose-Sepharose, a highly pure preparation of GNA can be obtained.}},
  author       = {{Van Damme, Els}},
  booktitle    = {{Lectin purification and analysis : methods and protocols}},
  isbn         = {{9781071604298}},
  issn         = {{1064-3745}},
  keywords     = {{Agglutination activity,Glycan binding,GNA,Plant lectin,Snowdrop}},
  language     = {{eng}},
  pages        = {{413--419}},
  publisher    = {{Humana}},
  series       = {{Methods in Molecular Biology}},
  title        = {{Purification of GNA-related lectins from natural sources}},
  url          = {{http://dx.doi.org/10.1007/978-1-0716-0430-4_40}},
  volume       = {{2132}},
  year         = {{2020}},
}

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