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Efficient Nitrogen-Fixing Bacteria Isolated from Soybean Nodules in the Semi-arid Region of Northeast Brazil are Classified as Bradyrhizobium brasilense (Symbiovar Sojae)

(2020) CURRENT MICROBIOLOGY. 77(8). p.1746-1755
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Abstract
Soybean (Glycine max L.) is an important legume that greatly benefits from inoculation with nitrogen-fixing bacteria. In a previous study, five efficient nitrogen-fixing bacterial strains, isolated from nodules of soybean inoculated with soil from semi-arid region, Northeast Brazil, were identified as a new group within the genus Bradyrhizobium. The taxonomic status of these strains was evaluated in this study. The phylogenetic analysis of the 16S rRNA gene showed the high similarity of the five strains to Bradyrhizobium brasilense UFLA03-321(T) (100%), B. pachyrhizi PAC48(T) (100%), B. ripae WR4(T) (100%), B. elkanii USDA 76(T) (99.91%), and B. macuxiense BR 10303(T) (99.91%). However, multilocus sequence analysis of the housekeeping genes atpD, dnaK, gyrB, recA, and rpoB, average nucleotide identity, and digital DNA-DNA hybridization analyses supported the classification of the group as B. brasilense. Some phenotypic characteristics allowed differentiating the five strains and the type strain of B. brasilense from the two neighboring species (B. pachyrhizi PAC48(T) and B. elkanii USDA 76(T)). The nodC and nifH genes' analyses showed that these strains belong to symbiovar sojae, together with B. elkanii (USDA 76(T)) and B. ferriligni (CCBAU 51502(T)). The present results support the classification of these five strains as Bradyrhizobium brasilense (symbiovar sojae).
Keywords
SP-NOV., GENETIC DIVERSITY, MAXIMUM-LIKELIHOOD, ROOT-NODULES, STRAINS, RHIZOBIA, LEGUMES, DNA, GENUS, LEGUMINOSAE

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MLA
da Costa, Elaine Martins, et al. “Efficient Nitrogen-Fixing Bacteria Isolated from Soybean Nodules in the Semi-Arid Region of Northeast Brazil Are Classified as Bradyrhizobium Brasilense (Symbiovar Sojae).” CURRENT MICROBIOLOGY, vol. 77, no. 8, 2020, pp. 1746–55, doi:10.1007/s00284-020-01993-6.
APA
da Costa, E. M., Almeida Ribeiro, P. R., de Carvalho, T. S., Vicentin, R. P., Balsanelli, E., de Souza, E. M., … de Souza Moreira, F. M. (2020). Efficient Nitrogen-Fixing Bacteria Isolated from Soybean Nodules in the Semi-arid Region of Northeast Brazil are Classified as Bradyrhizobium brasilense (Symbiovar Sojae). CURRENT MICROBIOLOGY, 77(8), 1746–1755. https://doi.org/10.1007/s00284-020-01993-6
Chicago author-date
Costa, Elaine Martins da, Paula R. Almeida Ribeiro, Teotonio Soares de Carvalho, Rayssa Pereira Vicentin, Eduardo Balsanelli, Emanuel Maltempi de Souza, Liesbeth Lebbe, Anne Willems, and Fatima M. de Souza Moreira. 2020. “Efficient Nitrogen-Fixing Bacteria Isolated from Soybean Nodules in the Semi-Arid Region of Northeast Brazil Are Classified as Bradyrhizobium Brasilense (Symbiovar Sojae).” CURRENT MICROBIOLOGY 77 (8): 1746–55. https://doi.org/10.1007/s00284-020-01993-6.
Chicago author-date (all authors)
da Costa, Elaine Martins, Paula R. Almeida Ribeiro, Teotonio Soares de Carvalho, Rayssa Pereira Vicentin, Eduardo Balsanelli, Emanuel Maltempi de Souza, Liesbeth Lebbe, Anne Willems, and Fatima M. de Souza Moreira. 2020. “Efficient Nitrogen-Fixing Bacteria Isolated from Soybean Nodules in the Semi-Arid Region of Northeast Brazil Are Classified as Bradyrhizobium Brasilense (Symbiovar Sojae).” CURRENT MICROBIOLOGY 77 (8): 1746–1755. doi:10.1007/s00284-020-01993-6.
Vancouver
1.
da Costa EM, Almeida Ribeiro PR, de Carvalho TS, Vicentin RP, Balsanelli E, de Souza EM, et al. Efficient Nitrogen-Fixing Bacteria Isolated from Soybean Nodules in the Semi-arid Region of Northeast Brazil are Classified as Bradyrhizobium brasilense (Symbiovar Sojae). CURRENT MICROBIOLOGY. 2020;77(8):1746–55.
IEEE
[1]
E. M. da Costa et al., “Efficient Nitrogen-Fixing Bacteria Isolated from Soybean Nodules in the Semi-arid Region of Northeast Brazil are Classified as Bradyrhizobium brasilense (Symbiovar Sojae),” CURRENT MICROBIOLOGY, vol. 77, no. 8, pp. 1746–1755, 2020.
@article{8694886,
  abstract     = {Soybean (Glycine max L.) is an important legume that greatly benefits from inoculation with nitrogen-fixing bacteria. In a previous study, five efficient nitrogen-fixing bacterial strains, isolated from nodules of soybean inoculated with soil from semi-arid region, Northeast Brazil, were identified as a new group within the genus Bradyrhizobium. The taxonomic status of these strains was evaluated in this study. The phylogenetic analysis of the 16S rRNA gene showed the high similarity of the five strains to Bradyrhizobium brasilense UFLA03-321(T) (100%), B. pachyrhizi PAC48(T) (100%), B. ripae WR4(T) (100%), B. elkanii USDA 76(T) (99.91%), and B. macuxiense BR 10303(T) (99.91%). However, multilocus sequence analysis of the housekeeping genes atpD, dnaK, gyrB, recA, and rpoB, average nucleotide identity, and digital DNA-DNA hybridization analyses supported the classification of the group as B. brasilense. Some phenotypic characteristics allowed differentiating the five strains and the type strain of B. brasilense from the two neighboring species (B. pachyrhizi PAC48(T) and B. elkanii USDA 76(T)). The nodC and nifH genes' analyses showed that these strains belong to symbiovar sojae, together with B. elkanii (USDA 76(T)) and B. ferriligni (CCBAU 51502(T)). The present results support the classification of these five strains as Bradyrhizobium brasilense (symbiovar sojae).},
  author       = {da Costa, Elaine Martins and Almeida Ribeiro, Paula R. and de Carvalho, Teotonio Soares and Vicentin, Rayssa Pereira and Balsanelli, Eduardo and de Souza, Emanuel Maltempi and Lebbe, Liesbeth and Willems, Anne and de Souza Moreira, Fatima M.},
  issn         = {0343-8651},
  journal      = {CURRENT MICROBIOLOGY},
  keywords     = {SP-NOV.,GENETIC DIVERSITY,MAXIMUM-LIKELIHOOD,ROOT-NODULES,STRAINS,RHIZOBIA,LEGUMES,DNA,GENUS,LEGUMINOSAE},
  language     = {eng},
  number       = {8},
  pages        = {1746--1755},
  title        = {Efficient Nitrogen-Fixing Bacteria Isolated from Soybean Nodules in the Semi-arid Region of Northeast Brazil are Classified as Bradyrhizobium brasilense (Symbiovar Sojae)},
  url          = {http://dx.doi.org/10.1007/s00284-020-01993-6},
  volume       = {77},
  year         = {2020},
}

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