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An inside job : applications of intracellular single domain antibodies

Eline Soetens (UGent) , Marlies Ballegeer (UGent) and Xavier Saelens (UGent)
(2020) BIOMOLECULES. 10(12).
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Abstract
Sera of camelid species contain a special kind of antibody that consists only of heavy chains. The variable antigen binding domain of these heavy chain antibodies can be expressed as a separate entity, called a single domain antibody that is characterized by its small size, high solubility and oftentimes exceptional stability. Because of this, most single domain antibodies fold correctly when expressed in the reducing environment of the cytoplasm, and thereby retain their antigen binding specificity. Single domain antibodies can thus be used to target a broad range of intracellular proteins. Such intracellular single domain antibodies are also known as intrabodies, and have proven to be highly useful tools for basic research by allowing visualization, disruption and even targeted degradation of intracellular proteins. Furthermore, intrabodies can be used to uncover prospective new therapeutic targets and have the potential to be applied in therapeutic settings in the future. In this review we provide a brief overview of recent advances in the field of intracellular single domain antibodies, focusing on their use as research tools and potential therapeutic applications. Special attention is given to the available methods that allow delivery of single domain antibodies into cells.
Keywords
single domain antibody, intrabody, delivery methods, therapy, research tool, CELL-PENETRATING PEPTIDES, OCULOPHARYNGEAL MUSCULAR-DYSTROPHY, AFFINITY MATURATION, MONOCLONAL-ANTIBODY, MAMMALIAN-CELLS, IN-VITRO, HCV REPLICATION, PROTEIN, DELIVERY, NANOBODIES

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Citation

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MLA
Soetens, Eline, et al. “An inside Job : Applications of Intracellular Single Domain Antibodies.” BIOMOLECULES, vol. 10, no. 12, 2020, doi:10.3390/biom10121663.
APA
Soetens, E., Ballegeer, M., & Saelens, X. (2020). An inside job : applications of intracellular single domain antibodies. BIOMOLECULES, 10(12). https://doi.org/10.3390/biom10121663
Chicago author-date
Soetens, Eline, Marlies Ballegeer, and Xavier Saelens. 2020. “An inside Job : Applications of Intracellular Single Domain Antibodies.” BIOMOLECULES 10 (12). https://doi.org/10.3390/biom10121663.
Chicago author-date (all authors)
Soetens, Eline, Marlies Ballegeer, and Xavier Saelens. 2020. “An inside Job : Applications of Intracellular Single Domain Antibodies.” BIOMOLECULES 10 (12). doi:10.3390/biom10121663.
Vancouver
1.
Soetens E, Ballegeer M, Saelens X. An inside job : applications of intracellular single domain antibodies. BIOMOLECULES. 2020;10(12).
IEEE
[1]
E. Soetens, M. Ballegeer, and X. Saelens, “An inside job : applications of intracellular single domain antibodies,” BIOMOLECULES, vol. 10, no. 12, 2020.
@article{8693886,
  abstract     = {{Sera of camelid species contain a special kind of antibody that consists only of heavy chains. The variable antigen binding domain of these heavy chain antibodies can be expressed as a separate entity, called a single domain antibody that is characterized by its small size, high solubility and oftentimes exceptional stability. Because of this, most single domain antibodies fold correctly when expressed in the reducing environment of the cytoplasm, and thereby retain their antigen binding specificity. Single domain antibodies can thus be used to target a broad range of intracellular proteins. Such intracellular single domain antibodies are also known as intrabodies, and have proven to be highly useful tools for basic research by allowing visualization, disruption and even targeted degradation of intracellular proteins. Furthermore, intrabodies can be used to uncover prospective new therapeutic targets and have the potential to be applied in therapeutic settings in the future. In this review we provide a brief overview of recent advances in the field of intracellular single domain antibodies, focusing on their use as research tools and potential therapeutic applications. Special attention is given to the available methods that allow delivery of single domain antibodies into cells.}},
  articleno    = {{1663}},
  author       = {{Soetens, Eline and Ballegeer, Marlies and Saelens, Xavier}},
  issn         = {{2218-273X}},
  journal      = {{BIOMOLECULES}},
  keywords     = {{single domain antibody,intrabody,delivery methods,therapy,research tool,CELL-PENETRATING PEPTIDES,OCULOPHARYNGEAL MUSCULAR-DYSTROPHY,AFFINITY MATURATION,MONOCLONAL-ANTIBODY,MAMMALIAN-CELLS,IN-VITRO,HCV REPLICATION,PROTEIN,DELIVERY,NANOBODIES}},
  language     = {{eng}},
  number       = {{12}},
  pages        = {{30}},
  title        = {{An inside job : applications of intracellular single domain antibodies}},
  url          = {{http://doi.org/10.3390/biom10121663}},
  volume       = {{10}},
  year         = {{2020}},
}
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