Advanced search
5 files | 3.23 MB Add to list

Rewired phenolic metabolism and improved saccharification efficiency of a Zea mays cinnamyl alcohol dehydrogenase 2 (zmcad2) mutant

Xinyu Liu (UGent) , Rebecca Van Acker (UGent) , Wannes Voorend (UGent) , Andreas Pallidis (UGent) , Geert Goeminne (UGent) , Jacob Pollier (UGent) , Kris Morreel (UGent) , Hoon Kim, Hilde Muylle (UGent) , Mickael Bosio, et al.
Author
Organization
Abstract
Lignocellulosic biomass is an abundant byproduct from cereal crops that can potentially be valorized as a feedstock to produce biomaterials. Zea mays CINNAMYL ALCOHOL DEHYDROGENASE 2 (ZmCAD2) is involved in lignification, and is a promising target to improve the cellulose‐to‐glucose conversion of maize stover. Here, we analyzed a field‐grown zmcad2 Mutator transposon insertional mutant. Zmcad2 mutant plants had an 18% lower Klason lignin content, whereas their cellulose content was similar to that of control lines. The lignin in zmcad2 mutants contained increased levels of hydroxycinnamaldehydes, i.e. the substrates of ZmCAD2, ferulic acid and tricin. Ferulates decorating hemicelluloses were not altered. Phenolic profiling further revealed that hydroxycinnamaldehydes are partly converted into (dihydro)ferulic acid and sinapic acid and their derivatives in zmcad2 mutants. Syringyl lactic acid hexoside, a metabolic sink in CAD‐deficient dicot trees, appeared not to be a sink in zmcad2 maize. The enzymatic cellulose‐to‐glucose conversion efficiency was determined after 10 different thermochemical pre‐treatments. Zmcad2 yielded significantly higher conversions compared with controls for almost every pre‐treatment. However, the relative increase in glucose yields after alkaline pre‐treatment was not higher than the relative increase when no pre‐treatment was applied, suggesting that the positive effect of the incorporation of hydroxycinnamaldehydes was leveled off by the negative effect of reduced p‐coumarate levels in the cell wall. Taken together, our results reveal how phenolic metabolism is affected in CAD‐deficient maize, and further support mutating CAD genes in cereal crops as a promising strategy to improve lignocellulosic biomass for sugar‐platform biorefineries.
Keywords
Plant Science, Genetics, Cell Biology, lignin, CAD, brown midrib, bm1, maize, field-grown, saccharification, pre-treatment, metabolomics, phenolic profiling, CINNAMYL-ALCOHOL-DEHYDROGENASE, CELL-WALL COMPOSITION, LIGNIN BIOSYNTHETIC-PATHWAY, ACID O-METHYLTRANSFERASE, HOT-WATER PRETREATMENT, BROWN-MIDRIB MUTANTS, DOWN-REGULATION, STRUCTURAL-CHARACTERIZATION, FERULIC ACID, SATIVA L.

Downloads

  • (...).xlsx
    • supplementary material
    • |
    • UGent only
    • |
    • Excel
    • |
    • 12.56 KB
  • (...).xlsx
    • supplementary material
    • |
    • UGent only
    • |
    • Excel
    • |
    • 13.99 KB
  • (...).pdf
    • supplementary material
    • |
    • UGent only
    • |
    • PDF
    • |
    • 929.36 KB
  • (...).pdf
    • supplementary material
    • |
    • UGent only
    • |
    • PDF
    • |
    • 712.41 KB
  • (...).pdf
    • full text (Accepted manuscript)
    • |
    • UGent only
    • |
    • PDF
    • |
    • 1.57 MB

Citation

Please use this url to cite or link to this publication:

MLA
Liu, Xinyu, et al. “Rewired Phenolic Metabolism and Improved Saccharification Efficiency of a Zea Mays Cinnamyl Alcohol Dehydrogenase 2 (Zmcad2) Mutant.” PLANT JOURNAL, 2021, doi:10.1111/tpj.15108.
APA
Liu, X., Van Acker, R., Voorend, W., Pallidis, A., Goeminne, G., Pollier, J., … Boerjan, W. (2021). Rewired phenolic metabolism and improved saccharification efficiency of a Zea mays cinnamyl alcohol dehydrogenase 2 (zmcad2) mutant. PLANT JOURNAL. https://doi.org/10.1111/tpj.15108
Chicago author-date
Liu, Xinyu, Rebecca Van Acker, Wannes Voorend, Andreas Pallidis, Geert Goeminne, Jacob Pollier, Kris Morreel, et al. 2021. “Rewired Phenolic Metabolism and Improved Saccharification Efficiency of a Zea Mays Cinnamyl Alcohol Dehydrogenase 2 (Zmcad2) Mutant.” PLANT JOURNAL. https://doi.org/10.1111/tpj.15108.
Chicago author-date (all authors)
Liu, Xinyu, Rebecca Van Acker, Wannes Voorend, Andreas Pallidis, Geert Goeminne, Jacob Pollier, Kris Morreel, Hoon Kim, Hilde Muylle, Mickael Bosio, John Ralph, Ruben Vanholme, and Wout Boerjan. 2021. “Rewired Phenolic Metabolism and Improved Saccharification Efficiency of a Zea Mays Cinnamyl Alcohol Dehydrogenase 2 (Zmcad2) Mutant.” PLANT JOURNAL. doi:10.1111/tpj.15108.
Vancouver
1.
Liu X, Van Acker R, Voorend W, Pallidis A, Goeminne G, Pollier J, et al. Rewired phenolic metabolism and improved saccharification efficiency of a Zea mays cinnamyl alcohol dehydrogenase 2 (zmcad2) mutant. PLANT JOURNAL. 2021;
IEEE
[1]
X. Liu et al., “Rewired phenolic metabolism and improved saccharification efficiency of a Zea mays cinnamyl alcohol dehydrogenase 2 (zmcad2) mutant,” PLANT JOURNAL, 2021.
@article{8693445,
  abstract     = {{Lignocellulosic biomass is an abundant byproduct from cereal crops that can potentially be valorized as a feedstock to produce biomaterials. Zea mays CINNAMYL ALCOHOL DEHYDROGENASE 2 (ZmCAD2) is involved in lignification, and is a promising target to improve the cellulose‐to‐glucose conversion of maize stover. Here, we analyzed a field‐grown zmcad2 Mutator transposon insertional mutant. Zmcad2 mutant plants had an 18% lower Klason lignin content, whereas their cellulose content was similar to that of control lines. The lignin in zmcad2 mutants contained increased levels of hydroxycinnamaldehydes, i.e. the substrates of ZmCAD2, ferulic acid and tricin. Ferulates decorating hemicelluloses were not altered. Phenolic profiling further revealed that hydroxycinnamaldehydes are partly converted into (dihydro)ferulic acid and sinapic acid and their derivatives in zmcad2 mutants. Syringyl lactic acid hexoside, a metabolic sink in CAD‐deficient dicot trees, appeared not to be a sink in zmcad2 maize. The enzymatic cellulose‐to‐glucose conversion efficiency was determined after 10 different thermochemical pre‐treatments. Zmcad2 yielded significantly higher conversions compared with controls for almost every pre‐treatment. However, the relative increase in glucose yields after alkaline pre‐treatment was not higher than the relative increase when no pre‐treatment was applied, suggesting that the positive effect of the incorporation of hydroxycinnamaldehydes was leveled off by the negative effect of reduced p‐coumarate levels in the cell wall. Taken together, our results reveal how phenolic metabolism is affected in CAD‐deficient maize, and further support mutating CAD genes in cereal crops as a promising strategy to improve lignocellulosic biomass for sugar‐platform biorefineries.}},
  author       = {{Liu, Xinyu and Van Acker, Rebecca and Voorend, Wannes and Pallidis, Andreas and Goeminne, Geert and Pollier, Jacob and Morreel, Kris and Kim, Hoon and Muylle, Hilde and Bosio, Mickael and Ralph, John and Vanholme, Ruben and Boerjan, Wout}},
  issn         = {{0960-7412}},
  journal      = {{PLANT JOURNAL}},
  keywords     = {{Plant Science,Genetics,Cell Biology,lignin,CAD,brown midrib,bm1,maize,field-grown,saccharification,pre-treatment,metabolomics,phenolic profiling,CINNAMYL-ALCOHOL-DEHYDROGENASE,CELL-WALL COMPOSITION,LIGNIN BIOSYNTHETIC-PATHWAY,ACID O-METHYLTRANSFERASE,HOT-WATER PRETREATMENT,BROWN-MIDRIB MUTANTS,DOWN-REGULATION,STRUCTURAL-CHARACTERIZATION,FERULIC ACID,SATIVA L.}},
  language     = {{eng}},
  pages        = {{18}},
  title        = {{Rewired phenolic metabolism and improved saccharification efficiency of a Zea mays cinnamyl alcohol dehydrogenase 2 (zmcad2) mutant}},
  url          = {{http://dx.doi.org/10.1111/tpj.15108}},
  year         = {{2021}},
}

Altmetric
View in Altmetric
Web of Science
Times cited: