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Prevalence, antimicrobial resistance, and molecular characterization of salmonella in cattle, beef, and diarrheic patients in Bishoftu, Ethiopia

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Abstract
Within Ethiopia, there is a lack of information on the genetic relatedness of Salmonella from cattle, beef, and diarrheic patients and its potential transmission from cattle to humans through consumption of contaminated beef. The objective of this study was to assess the prevalence and determine the serotypes, genetic relatedness, and antimicrobial resistance of Salmonella in cattle in two local slaughterhouses, in beef at retail shops, and in diarrheic patients in the only hospital in Bishoftu, Ethiopia. Salmonella was detected in 2.5% (6/240) of cattle samples, in 8.7% (11/127) of beef samples, and in 2.3% (5/216) of the diarrheic patients. Four Salmonella serotypes: Salmonella Typhimurium, Salmonella Eastbourne, Salmonella Saintpaul, and Salmonella Cotham were identified. Salmonella Typhimurium and Salmonella Eastbourne were isolated from cattle and beef, whereas Salmonella Saintpaul and Salmonella Cotham were isolated only from diarrheic patients. Except for serotype Salmonella Saintpaul, all isolates were grouped into five pulsotypes, of which two pulsotypes contained isolates from cattle and beef. Isolates from humans represented unique pulsotypes. Among the 22 Salmonella isolates tested, 95.5% were resistant to at least 1 of the 14 antimicrobials tested. Three Salmonella isolates originating from cattle were multidrug resistant. One human isolate was susceptible to all antimicrobials tested. More specifically, resistance to ampicillin, sulfamethoxazole, tetracycline, tigecycline, and trimethoprim were observed. The most frequently observed resistance was to sulfamethoxazole (90.9%, 20/22) followed by trimethoprim (22.7%, 5/22). The study revealed considerable Salmonella contamination of beef at retail shops, antimicrobial resistance to commonly used antimicrobials, and shared genetically similar Salmonella serotypes between cattle and beef; the link with humans could not be established. Still, the findings of Salmonella in cattle and beef, the propensity of transfer of Salmonella from cattle to beef coupled with the common consumption of raw/undercooked beef are likely to pose public health risk in Ethiopia.
Keywords
Food Science, Animal Science and Zoology, Applied Microbiology and Biotechnology, Microbiology, Salmonella, antimicrobial resistance, genetic relatedness, cattle, beef, humans

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MLA
Desissa Gutema, Fanta, et al. “Prevalence, Antimicrobial Resistance, and Molecular Characterization of Salmonella in Cattle, Beef, and Diarrheic Patients in Bishoftu, Ethiopia.” FOODBORNE PATHOGENS AND DISEASE, vol. 18, no. 4, 2021, pp. 283–89, doi:10.1089/fpd.2020.2869.
APA
Desissa Gutema, F., Rasschaert, G., Agga, G. E., Merera, O., Duguma, A. B., Abdi, R. D., … De Zutter, L. (2021). Prevalence, antimicrobial resistance, and molecular characterization of salmonella in cattle, beef, and diarrheic patients in Bishoftu, Ethiopia. FOODBORNE PATHOGENS AND DISEASE, 18(4), 283–289. https://doi.org/10.1089/fpd.2020.2869
Chicago author-date
Desissa Gutema, Fanta, Geertrui Rasschaert, Getahun E. Agga, Olana Merera, Addisu B. Duguma, Reta D. Abdi, Luc Duchateau, Wesley Mattheus, Sarah Gabriël, and Lieven De Zutter. 2021. “Prevalence, Antimicrobial Resistance, and Molecular Characterization of Salmonella in Cattle, Beef, and Diarrheic Patients in Bishoftu, Ethiopia.” FOODBORNE PATHOGENS AND DISEASE 18 (4): 283–89. https://doi.org/10.1089/fpd.2020.2869.
Chicago author-date (all authors)
Desissa Gutema, Fanta, Geertrui Rasschaert, Getahun E. Agga, Olana Merera, Addisu B. Duguma, Reta D. Abdi, Luc Duchateau, Wesley Mattheus, Sarah Gabriël, and Lieven De Zutter. 2021. “Prevalence, Antimicrobial Resistance, and Molecular Characterization of Salmonella in Cattle, Beef, and Diarrheic Patients in Bishoftu, Ethiopia.” FOODBORNE PATHOGENS AND DISEASE 18 (4): 283–289. doi:10.1089/fpd.2020.2869.
Vancouver
1.
Desissa Gutema F, Rasschaert G, Agga GE, Merera O, Duguma AB, Abdi RD, et al. Prevalence, antimicrobial resistance, and molecular characterization of salmonella in cattle, beef, and diarrheic patients in Bishoftu, Ethiopia. FOODBORNE PATHOGENS AND DISEASE. 2021;18(4):283–9.
IEEE
[1]
F. Desissa Gutema et al., “Prevalence, antimicrobial resistance, and molecular characterization of salmonella in cattle, beef, and diarrheic patients in Bishoftu, Ethiopia,” FOODBORNE PATHOGENS AND DISEASE, vol. 18, no. 4, pp. 283–289, 2021.
@article{8693438,
  abstract     = {{Within Ethiopia, there is a lack of information on the genetic relatedness of Salmonella from cattle, beef, and diarrheic patients and its potential transmission from cattle to humans through consumption of contaminated beef. The objective of this study was to assess the prevalence and determine the serotypes, genetic relatedness, and antimicrobial resistance of Salmonella in cattle in two local slaughterhouses, in beef at retail shops, and in diarrheic patients in the only hospital in Bishoftu, Ethiopia. Salmonella was detected in 2.5% (6/240) of cattle samples, in 8.7% (11/127) of beef samples, and in 2.3% (5/216) of the diarrheic patients. Four Salmonella serotypes: Salmonella Typhimurium, Salmonella Eastbourne, Salmonella Saintpaul, and Salmonella Cotham were identified. Salmonella Typhimurium and Salmonella Eastbourne were isolated from cattle and beef, whereas Salmonella Saintpaul and Salmonella Cotham were isolated only from diarrheic patients. Except for serotype Salmonella Saintpaul, all isolates were grouped into five pulsotypes, of which two pulsotypes contained isolates from cattle and beef. Isolates from humans represented unique pulsotypes. Among the 22 Salmonella isolates tested, 95.5% were resistant to at least 1 of the 14 antimicrobials tested. Three Salmonella isolates originating from cattle were multidrug resistant. One human isolate was susceptible to all antimicrobials tested. More specifically, resistance to ampicillin, sulfamethoxazole, tetracycline, tigecycline, and trimethoprim were observed. The most frequently observed resistance was to sulfamethoxazole (90.9%, 20/22) followed by trimethoprim (22.7%, 5/22). The study revealed considerable Salmonella contamination of beef at retail shops, antimicrobial resistance to commonly used antimicrobials, and shared genetically similar Salmonella serotypes between cattle and beef; the link with humans could not be established. Still, the findings of Salmonella in cattle and beef, the propensity of transfer of Salmonella from cattle to beef coupled with the common consumption of raw/undercooked beef are likely to pose public health risk in Ethiopia.}},
  author       = {{Desissa Gutema, Fanta and Rasschaert, Geertrui and Agga, Getahun E. and Merera, Olana and Duguma, Addisu B. and Abdi, Reta D. and Duchateau, Luc and Mattheus, Wesley and Gabriël, Sarah and De Zutter, Lieven}},
  issn         = {{1535-3141}},
  journal      = {{FOODBORNE PATHOGENS AND DISEASE}},
  keywords     = {{Food Science,Animal Science and Zoology,Applied Microbiology and Biotechnology,Microbiology,Salmonella,antimicrobial resistance,genetic relatedness,cattle,beef,humans}},
  language     = {{eng}},
  number       = {{4}},
  pages        = {{283--289}},
  title        = {{Prevalence, antimicrobial resistance, and molecular characterization of salmonella in cattle, beef, and diarrheic patients in Bishoftu, Ethiopia}},
  url          = {{http://dx.doi.org/10.1089/fpd.2020.2869}},
  volume       = {{18}},
  year         = {{2021}},
}

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