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Quantitative lipidomic analysis of Ascaris suum

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Abstract
Ascaris is a soil-transmitted nematode that causes ascariasis, a neglected tropical disease affecting predominantly children and adolescents in the tropics and subtropics. Approximately 0.8 billion people are affected worldwide, equating to 0.86 million disability-adjusted life-years (DALYs). Exploring the molecular biology of Ascaris is important to gain a better understanding of the host-parasite interactions and disease processes, and supports the development of novel interventions. Although advances have been made in the genomics, transcriptomics and proteomics of Ascaris, its lipidome has received very limited attention. Lipidomics is an important sub-discipline of systems biology, focused on exploring lipids profiles in tissues and cells, and elucidating their biological and metabolic roles. Here, we characterised the lipidomes of key developmental stages and organ systems of Ascaris of porcine origin via high throughput LC-MS/MS. In total, > 500 lipid species belonging to 18 lipid classes within three lipid categories were identified and quantified-in precise molar amounts in relation to the dry weight of worm material-in different developmental stages/sexes and organ systems. The results showed substantial differences in the composition and abundance of lipids with key roles in cellular processes and functions (e.g. energy storage regulation and membrane structure) among distinct stages and among organ systems, likely reflecting differing demands for lipids, depending on stage of growth and development as well as the need to adapt to constantly changing environments within and outside of the host animal. This work provides the first step toward understanding the biology of lipids in Ascaris, with possibilities to work toward designing new interventions against ascariasis. Author summary Lipids are of vital importance in the biology of parasitic worms, particularly in relation to cellular membranes, energy storage, and intra- and intercellular signalling. However, very little is known about the biology of lipids in parasitic nematodes. Using a high-throughput LC-MS/MS approach, we characterised the first global lipidome for Ascaris. We investigated the lipid composition and abundance in key developmental stages/sexes as well as the organ systems of Ascaris. We observed substantial differences in lipid composition and abundance among these stages/sexes and among the organ systems studied. The findings provide a basis to start to understand lipid biology in Ascaris, with possible implications for developing new interventions against ascariasis.
Keywords
MASS-SPECTROMETRY, GLYOXYLATE CYCLE, DNA ELIMINATION, LUMBRICOIDES, METABOLISM, PLATFORM, PERSEUS, LARVAE

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MLA
Wang, Tao, et al. “Quantitative Lipidomic Analysis of Ascaris Suum.” PLOS NEGLECTED TROPICAL DISEASES, vol. 14, no. 12, 2020, doi:10.1371/journal.pntd.0008848.
APA
Wang, T., Nie, S., Ma, G., Vlaminck, J., Geldhof, P., Williamson, N. A., … Gasser, R. B. (2020). Quantitative lipidomic analysis of Ascaris suum. PLOS NEGLECTED TROPICAL DISEASES, 14(12). https://doi.org/10.1371/journal.pntd.0008848
Chicago author-date
Wang, Tao, Shuai Nie, Guangxu Ma, Johnny Vlaminck, Peter Geldhof, Nicholas A. Williamson, Gavin E. Reid, and Robin B. Gasser. 2020. “Quantitative Lipidomic Analysis of Ascaris Suum.” PLOS NEGLECTED TROPICAL DISEASES 14 (12). https://doi.org/10.1371/journal.pntd.0008848.
Chicago author-date (all authors)
Wang, Tao, Shuai Nie, Guangxu Ma, Johnny Vlaminck, Peter Geldhof, Nicholas A. Williamson, Gavin E. Reid, and Robin B. Gasser. 2020. “Quantitative Lipidomic Analysis of Ascaris Suum.” PLOS NEGLECTED TROPICAL DISEASES 14 (12). doi:10.1371/journal.pntd.0008848.
Vancouver
1.
Wang T, Nie S, Ma G, Vlaminck J, Geldhof P, Williamson NA, et al. Quantitative lipidomic analysis of Ascaris suum. PLOS NEGLECTED TROPICAL DISEASES. 2020;14(12).
IEEE
[1]
T. Wang et al., “Quantitative lipidomic analysis of Ascaris suum,” PLOS NEGLECTED TROPICAL DISEASES, vol. 14, no. 12, 2020.
@article{8692929,
  abstract     = {{Ascaris is a soil-transmitted nematode that causes ascariasis, a neglected tropical disease affecting predominantly children and adolescents in the tropics and subtropics. Approximately 0.8 billion people are affected worldwide, equating to 0.86 million disability-adjusted life-years (DALYs). Exploring the molecular biology of Ascaris is important to gain a better understanding of the host-parasite interactions and disease processes, and supports the development of novel interventions. Although advances have been made in the genomics, transcriptomics and proteomics of Ascaris, its lipidome has received very limited attention. Lipidomics is an important sub-discipline of systems biology, focused on exploring lipids profiles in tissues and cells, and elucidating their biological and metabolic roles. Here, we characterised the lipidomes of key developmental stages and organ systems of Ascaris of porcine origin via high throughput LC-MS/MS. In total, > 500 lipid species belonging to 18 lipid classes within three lipid categories were identified and quantified-in precise molar amounts in relation to the dry weight of worm material-in different developmental stages/sexes and organ systems. The results showed substantial differences in the composition and abundance of lipids with key roles in cellular processes and functions (e.g. energy storage regulation and membrane structure) among distinct stages and among organ systems, likely reflecting differing demands for lipids, depending on stage of growth and development as well as the need to adapt to constantly changing environments within and outside of the host animal. This work provides the first step toward understanding the biology of lipids in Ascaris, with possibilities to work toward designing new interventions against ascariasis. Author summary Lipids are of vital importance in the biology of parasitic worms, particularly in relation to cellular membranes, energy storage, and intra- and intercellular signalling. However, very little is known about the biology of lipids in parasitic nematodes. Using a high-throughput LC-MS/MS approach, we characterised the first global lipidome for Ascaris. We investigated the lipid composition and abundance in key developmental stages/sexes as well as the organ systems of Ascaris. We observed substantial differences in lipid composition and abundance among these stages/sexes and among the organ systems studied. The findings provide a basis to start to understand lipid biology in Ascaris, with possible implications for developing new interventions against ascariasis.}},
  articleno    = {{e0008848}},
  author       = {{Wang, Tao and Nie, Shuai and Ma, Guangxu and Vlaminck, Johnny and Geldhof, Peter and Williamson, Nicholas A. and Reid, Gavin E. and Gasser, Robin B.}},
  issn         = {{1935-2735}},
  journal      = {{PLOS NEGLECTED TROPICAL DISEASES}},
  keywords     = {{MASS-SPECTROMETRY,GLYOXYLATE CYCLE,DNA ELIMINATION,LUMBRICOIDES,METABOLISM,PLATFORM,PERSEUS,LARVAE}},
  language     = {{eng}},
  number       = {{12}},
  pages        = {{19}},
  title        = {{Quantitative lipidomic analysis of Ascaris suum}},
  url          = {{http://doi.org/10.1371/journal.pntd.0008848}},
  volume       = {{14}},
  year         = {{2020}},
}

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