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Early pH changes in musculoskeletal tissues upon injury-aerobic catabolic pathway activity linked to inter-individual differences in local pH

Julia C. Berkmann, Aaron X. Herrera Martin, Agnes Ellinghaus, Claudia Schlundt, Hanna Schell, Evi Lippens (UGent) , Georg N. Duda, Serafeim Tsitsilonis and Katharina Schmidt-Bleek
(2020) INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. 21(7).
Author
Organization
Abstract
Local pH is stated to acidify after bone fracture. However, the time course and degree of acidification remain unknown. Whether the acidification pattern within a fracture hematoma is applicable to adjacent muscle hematoma or is exclusive to this regenerative tissue has not been studied to date. Thus, in this study, we aimed to unravel the extent and pattern of acidification in vivo during the early phase post musculoskeletal injury. Local pH changes after fracture and muscle trauma were measured simultaneously in two pre-clinical animal models (sheep/rats) immediately after and up to 48 h post injury. The rat fracture hematoma was further analyzed histologically and metabolomically. In vivo pH measurements in bone and muscle hematoma revealed a local acidification in both animal models, yielding mean pH values in rats of 6.69 and 6.89, with pronounced intra- and inter-individual differences. The metabolomic analysis of the hematomas indicated a link between reduction in tricarboxylic acid cycle activity and pH, thus, metabolic activity within the injured tissues could be causative for the different pH values. The significant acidification within the early musculoskeletal hematoma could enable the employment of the pH for novel, sought-after treatments that allow for spatially and temporally controlled drug release.
Keywords
FRACTURE HEMATOMA, BONE, ACIDOSIS, ENHANCEMENT, BICARBONATE, METABOLISM, SENSORS, SYSTEM, CELLS, RISK, pH change, musculoskeletal system, bone healing, muscle injury, initial, healing phase, pH-triggered drug release

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MLA
Berkmann, Julia C., et al. “Early PH Changes in Musculoskeletal Tissues upon Injury-Aerobic Catabolic Pathway Activity Linked to Inter-Individual Differences in Local PH.” INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, vol. 21, no. 7, 2020, doi:10.3390/ijms21072513.
APA
Berkmann, J. C., Martin, A. X. H., Ellinghaus, A., Schlundt, C., Schell, H., Lippens, E., … Schmidt-Bleek, K. (2020). Early pH changes in musculoskeletal tissues upon injury-aerobic catabolic pathway activity linked to inter-individual differences in local pH. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 21(7). https://doi.org/10.3390/ijms21072513
Chicago author-date
Berkmann, Julia C., Aaron X. Herrera Martin, Agnes Ellinghaus, Claudia Schlundt, Hanna Schell, Evi Lippens, Georg N. Duda, Serafeim Tsitsilonis, and Katharina Schmidt-Bleek. 2020. “Early PH Changes in Musculoskeletal Tissues upon Injury-Aerobic Catabolic Pathway Activity Linked to Inter-Individual Differences in Local PH.” INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 21 (7). https://doi.org/10.3390/ijms21072513.
Chicago author-date (all authors)
Berkmann, Julia C., Aaron X. Herrera Martin, Agnes Ellinghaus, Claudia Schlundt, Hanna Schell, Evi Lippens, Georg N. Duda, Serafeim Tsitsilonis, and Katharina Schmidt-Bleek. 2020. “Early PH Changes in Musculoskeletal Tissues upon Injury-Aerobic Catabolic Pathway Activity Linked to Inter-Individual Differences in Local PH.” INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 21 (7). doi:10.3390/ijms21072513.
Vancouver
1.
Berkmann JC, Martin AXH, Ellinghaus A, Schlundt C, Schell H, Lippens E, et al. Early pH changes in musculoskeletal tissues upon injury-aerobic catabolic pathway activity linked to inter-individual differences in local pH. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. 2020;21(7).
IEEE
[1]
J. C. Berkmann et al., “Early pH changes in musculoskeletal tissues upon injury-aerobic catabolic pathway activity linked to inter-individual differences in local pH,” INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, vol. 21, no. 7, 2020.
@article{8692530,
  abstract     = {{Local pH is stated to acidify after bone fracture. However, the time course and degree of acidification remain unknown. Whether the acidification pattern within a fracture hematoma is applicable to adjacent muscle hematoma or is exclusive to this regenerative tissue has not been studied to date. Thus, in this study, we aimed to unravel the extent and pattern of acidification in vivo during the early phase post musculoskeletal injury. Local pH changes after fracture and muscle trauma were measured simultaneously in two pre-clinical animal models (sheep/rats) immediately after and up to 48 h post injury. The rat fracture hematoma was further analyzed histologically and metabolomically. In vivo pH measurements in bone and muscle hematoma revealed a local acidification in both animal models, yielding mean pH values in rats of 6.69 and 6.89, with pronounced intra- and inter-individual differences. The metabolomic analysis of the hematomas indicated a link between reduction in tricarboxylic acid cycle activity and pH, thus, metabolic activity within the injured tissues could be causative for the different pH values. The significant acidification within the early musculoskeletal hematoma could enable the employment of the pH for novel, sought-after treatments that allow for spatially and temporally controlled drug release.}},
  articleno    = {{2513}},
  author       = {{Berkmann, Julia C. and Martin, Aaron X. Herrera and Ellinghaus, Agnes and Schlundt, Claudia and Schell, Hanna and Lippens, Evi and Duda, Georg N. and Tsitsilonis, Serafeim and Schmidt-Bleek, Katharina}},
  issn         = {{1422-0067}},
  journal      = {{INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES}},
  keywords     = {{FRACTURE HEMATOMA,BONE,ACIDOSIS,ENHANCEMENT,BICARBONATE,METABOLISM,SENSORS,SYSTEM,CELLS,RISK,pH change,musculoskeletal system,bone healing,muscle injury,initial,healing phase,pH-triggered drug release}},
  language     = {{eng}},
  number       = {{7}},
  pages        = {{18}},
  title        = {{Early pH changes in musculoskeletal tissues upon injury-aerobic catabolic pathway activity linked to inter-individual differences in local pH}},
  url          = {{http://doi.org/10.3390/ijms21072513}},
  volume       = {{21}},
  year         = {{2020}},
}
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