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Targeting a coatomer protein complex-I gene via RNA interference results in effective lethality in the pollen beetle Brassicogethes aeneus

(2021) JOURNAL OF PEST SCIENCE. 94(3). p.703-712
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Abstract
he pollen beetle Brassicogethes aeneus is a serious pest of oilseed rape (Brassica napus) in Europe. Management of this pest has grown difficult due to B. aeneus's development of resistance to pyrethroid insecticides, as well as the pressure to establish control strategies that minimise the impact on nontarget organisms. RNA interference represents a nucleotide sequence-based, and thus potentially species-specific, approach to agricultural pest control. The present study examined the efficacy of targeting the coatomer gene coatomer subunit alpha (alpha COP), via both microinjection and dietary exposure to exogenous complementary dsRNA, on alpha COP-silencing and subsequent mortality in B. aeneus. Beetles injected with dsRNA targeting alpha COP (at 0.14 mu g/mg) showed 88% and 100% mortality at 6 and 10 days post-injection, respectively; where by the same time after dietary exposure, 43%-89% mortality was observed in the 3 mu g dsRNA/mu L treatment, though the effect was concentration-dependent. Thus, the effect was significant for both delivery routes. In working towards RNA-based management of B. aeneus, future studies should include alpha COP as a target of interest.
Keywords
RNAi, Brassicogethes aeneus, Meligethes aeneus, Oilseed rape, Rapeseed, COPI, Biopesticide, Coleoptera, INSECTICIDES, POPULATIONS, MECHANISMS, RESISTANCE, WEEVIL, LARVAL, SYSTEM, DSRNA, PESTS

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MLA
Willow, Jonathan, et al. “Targeting a Coatomer Protein Complex-I Gene via RNA Interference Results in Effective Lethality in the Pollen Beetle Brassicogethes Aeneus.” JOURNAL OF PEST SCIENCE, vol. 94, no. 3, 2021, pp. 703–12, doi:10.1007/s10340-020-01288-6.
APA
Willow, J., Sulg, S., Taning, N. T. C., Silva, A. I., Christiaens, O., Kaasik, R., … Veromann, E. (2021). Targeting a coatomer protein complex-I gene via RNA interference results in effective lethality in the pollen beetle Brassicogethes aeneus. JOURNAL OF PEST SCIENCE, 94(3), 703–712. https://doi.org/10.1007/s10340-020-01288-6
Chicago author-date
Willow, Jonathan, Silva Sulg, Nji Tizi Clauvis Taning, Ana Isabel Silva, Olivier Christiaens, Riina Kaasik, Katterinne Prentice Muro, Gabor L. Lovei, Guy Smagghe, and Eve Veromann. 2021. “Targeting a Coatomer Protein Complex-I Gene via RNA Interference Results in Effective Lethality in the Pollen Beetle Brassicogethes Aeneus.” JOURNAL OF PEST SCIENCE 94 (3): 703–12. https://doi.org/10.1007/s10340-020-01288-6.
Chicago author-date (all authors)
Willow, Jonathan, Silva Sulg, Nji Tizi Clauvis Taning, Ana Isabel Silva, Olivier Christiaens, Riina Kaasik, Katterinne Prentice Muro, Gabor L. Lovei, Guy Smagghe, and Eve Veromann. 2021. “Targeting a Coatomer Protein Complex-I Gene via RNA Interference Results in Effective Lethality in the Pollen Beetle Brassicogethes Aeneus.” JOURNAL OF PEST SCIENCE 94 (3): 703–712. doi:10.1007/s10340-020-01288-6.
Vancouver
1.
Willow J, Sulg S, Taning NTC, Silva AI, Christiaens O, Kaasik R, et al. Targeting a coatomer protein complex-I gene via RNA interference results in effective lethality in the pollen beetle Brassicogethes aeneus. JOURNAL OF PEST SCIENCE. 2021;94(3):703–12.
IEEE
[1]
J. Willow et al., “Targeting a coatomer protein complex-I gene via RNA interference results in effective lethality in the pollen beetle Brassicogethes aeneus,” JOURNAL OF PEST SCIENCE, vol. 94, no. 3, pp. 703–712, 2021.
@article{8692215,
  abstract     = {{he pollen beetle Brassicogethes aeneus is a serious pest of oilseed rape (Brassica napus) in Europe. Management of this pest has grown difficult due to B. aeneus's development of resistance to pyrethroid insecticides, as well as the pressure to establish control strategies that minimise the impact on nontarget organisms. RNA interference represents a nucleotide sequence-based, and thus potentially species-specific, approach to agricultural pest control. The present study examined the efficacy of targeting the coatomer gene coatomer subunit alpha (alpha COP), via both microinjection and dietary exposure to exogenous complementary dsRNA, on alpha COP-silencing and subsequent mortality in B. aeneus. Beetles injected with dsRNA targeting alpha COP (at 0.14 mu g/mg) showed 88% and 100% mortality at 6 and 10 days post-injection, respectively; where by the same time after dietary exposure, 43%-89% mortality was observed in the 3 mu g dsRNA/mu L treatment, though the effect was concentration-dependent. Thus, the effect was significant for both delivery routes. In working towards RNA-based management of B. aeneus, future studies should include alpha COP as a target of interest.}},
  author       = {{Willow, Jonathan and Sulg, Silva and Taning, Nji Tizi Clauvis and Silva, Ana Isabel and Christiaens, Olivier and Kaasik, Riina and Prentice Muro, Katterinne and Lovei, Gabor L. and Smagghe, Guy and Veromann, Eve}},
  issn         = {{1612-4758}},
  journal      = {{JOURNAL OF PEST SCIENCE}},
  keywords     = {{RNAi,Brassicogethes aeneus,Meligethes aeneus,Oilseed rape,Rapeseed,COPI,Biopesticide,Coleoptera,INSECTICIDES,POPULATIONS,MECHANISMS,RESISTANCE,WEEVIL,LARVAL,SYSTEM,DSRNA,PESTS}},
  language     = {{eng}},
  number       = {{3}},
  pages        = {{703--712}},
  title        = {{Targeting a coatomer protein complex-I gene via RNA interference results in effective lethality in the pollen beetle Brassicogethes aeneus}},
  url          = {{http://dx.doi.org/10.1007/s10340-020-01288-6}},
  volume       = {{94}},
  year         = {{2021}},
}

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