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Analysing mouse skin cell behaviour under a non-thermal kHz plasma jet

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Abstract
Plasma jets are extensively used in biomedical applications, particularly for exploring cell viability behaviour. However, many experimental parameters influence the results, including jet characteristics, secondary liquid chemistry and protocols used, slowing research progress. A specific interest of the presented research was skin cell behaviour under a non-thermal kHz plasma jet—a so-called cold plasma jet—as a topical skin treatment. Our research was focused on in vitro mouse skin cell direct plasma treatment with argon as an operating gas. The research was complemented with detailed gas-phase diagnostics and liquid-phase chemical analysis of the plasma and plasma-treated medium, respectively. The obtained results showed that direct plasma jet treatment was very destructive, leading to low cell viability. Even with short treatment times (from 35 s to 60 s), apoptosis was observed for most L929 murine fibroblasts under approximately the same conditions. This behaviour was attributed to plasma species generated from direct treatment and the types of cell lines used. Importantly, the research exposed important points that should be taken under consideration for all further research in this field: the urgent need to upgrade and standardise existing plasma treatment protocols of cell lines; to monitor gas and liquid chemistries and to standardise plasma discharge parameters.
Keywords
atmospheric pressure plasma jet, cell viability, plasma-treated media, reactive species, optical emission spectroscopy

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MLA
Jurov, Andrea, et al. “Analysing Mouse Skin Cell Behaviour under a Non-Thermal KHz Plasma Jet.” APPLIED SCIENCES-BASEL, vol. 11, no. 3, 2021, doi:10.3390/app11031266.
APA
Jurov, A., Kos, Š., Hojnik, N., Sremački, I., Nikiforov, A., Leys, C., … Cvelbar, U. (2021). Analysing mouse skin cell behaviour under a non-thermal kHz plasma jet. APPLIED SCIENCES-BASEL, 11(3). https://doi.org/10.3390/app11031266
Chicago author-date
Jurov, Andrea, Špela Kos, Nataša Hojnik, Ivana Sremački, Anton Nikiforov, Christophe Leys, Gregor Serša, and Uroš Cvelbar. 2021. “Analysing Mouse Skin Cell Behaviour under a Non-Thermal KHz Plasma Jet.” APPLIED SCIENCES-BASEL 11 (3). https://doi.org/10.3390/app11031266.
Chicago author-date (all authors)
Jurov, Andrea, Špela Kos, Nataša Hojnik, Ivana Sremački, Anton Nikiforov, Christophe Leys, Gregor Serša, and Uroš Cvelbar. 2021. “Analysing Mouse Skin Cell Behaviour under a Non-Thermal KHz Plasma Jet.” APPLIED SCIENCES-BASEL 11 (3). doi:10.3390/app11031266.
Vancouver
1.
Jurov A, Kos Š, Hojnik N, Sremački I, Nikiforov A, Leys C, et al. Analysing mouse skin cell behaviour under a non-thermal kHz plasma jet. APPLIED SCIENCES-BASEL. 2021;11(3).
IEEE
[1]
A. Jurov et al., “Analysing mouse skin cell behaviour under a non-thermal kHz plasma jet,” APPLIED SCIENCES-BASEL, vol. 11, no. 3, 2021.
@article{8691001,
  abstract     = {{Plasma jets are extensively used in biomedical applications, particularly for exploring cell viability behaviour. However, many experimental parameters influence the results, including jet characteristics, secondary liquid chemistry and protocols used, slowing research progress. A specific interest of the presented research was skin cell behaviour under a non-thermal kHz plasma jet—a so-called cold plasma jet—as a topical skin treatment. Our research was focused on in vitro mouse skin cell direct plasma treatment with argon as an operating gas. The research was complemented with detailed gas-phase diagnostics and liquid-phase chemical analysis of the plasma and plasma-treated medium, respectively. The obtained results showed that direct plasma jet treatment was very destructive, leading to low cell viability. Even with short treatment times (from 35 s to 60 s), apoptosis was observed for most L929 murine fibroblasts under approximately the same conditions. This behaviour was attributed to plasma species generated from direct treatment and the types of cell lines used. Importantly, the research exposed important points that should be taken under consideration for all further research in this field: the urgent need to upgrade and standardise existing plasma treatment protocols of cell lines; to monitor gas and liquid chemistries and to standardise plasma discharge parameters.}},
  articleno    = {{1266}},
  author       = {{Jurov, Andrea and Kos, Špela and Hojnik, Nataša and Sremački, Ivana and Nikiforov, Anton and Leys, Christophe and Serša, Gregor and Cvelbar, Uroš}},
  issn         = {{2076-3417}},
  journal      = {{APPLIED SCIENCES-BASEL}},
  keywords     = {{atmospheric pressure plasma jet,cell viability,plasma-treated media,reactive species,optical emission spectroscopy}},
  language     = {{eng}},
  number       = {{3}},
  pages        = {{12}},
  title        = {{Analysing mouse skin cell behaviour under a non-thermal kHz plasma jet}},
  url          = {{http://doi.org/10.3390/app11031266}},
  volume       = {{11}},
  year         = {{2021}},
}

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