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Biological activity and antimicrobial property of Cu/a-C:H nanocomposites and nanolayered coatings on titanium substrates

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Abstract
Infection associated with titanium based implants remains the most serious problem in implant surgery hence it is important to find optimal strategies to prevent infections. In the present study, we investigated the surface properties, antibacterial activity and biocompatibility of nanocomposite coatings based on an amorphous hydrocarbon (a-C:H) film containing copper nanoparticles (CuNPs) deposited on Ti discs via a gas aggregation cluster source. Three different Cu/a-C:H coatings with approximately the same amount of embedded CuNPs with and without barrier a-C:H layer were fabricated. The obtained results revealed that different structures of the produced coatings have significantly different release rates of Cu ions from the coatings into the aqueous media. This subsequently influences the antibacterial efficiency and osteoblast cell viability of the treated coatings. Coatings with the highest number of CuNPs resulted in excellent antibacterial activity exhibiting approximately 4 log reduction of E.coli and S.aureus after 24 h incubation. The cytotoxicity study revealed that after 7 day cell seeding, even the coating with the highest Cu at.% (4 at.%) showed a cell viability of.90%. Consequently, the coating, formed with a properly tailored number of CuNPs and a-C:H barrier thickness offer a strong antibacterial effect without any harm to osteoblast cells.
Keywords
Antibacterial coatings, Implant infection, Biocompatibility, Ti implants, Controlled release, CARBON DLC COATINGS, COPPER NANOPARTICLES, TIO2 COATINGS, IN-VITRO, ANTIBACTERIAL, CYTOTOXICITY, OSTEOBLASTS, SCAFFOLDS, FILMS

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MLA
Thukkaram, Monica, et al. “Biological Activity and Antimicrobial Property of Cu/a-C:H Nanocomposites and Nanolayered Coatings on Titanium Substrates.” MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS, vol. 119, 2021, doi:10.1016/j.msec.2020.111513.
APA
Thukkaram, M., Vaidulych, M., Kylian, O., Rigole, P., Aliakbarshirazi, S., Asadian, M., … De Geyter, N. (2021). Biological activity and antimicrobial property of Cu/a-C:H nanocomposites and nanolayered coatings on titanium substrates. MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS, 119. https://doi.org/10.1016/j.msec.2020.111513
Chicago author-date
Thukkaram, Monica, Mykhailo Vaidulych, Ondrej Kylian, Petra Rigole, Sheida Aliakbarshirazi, Mahtab Asadian, Anton Nikiforov, et al. 2021. “Biological Activity and Antimicrobial Property of Cu/a-C:H Nanocomposites and Nanolayered Coatings on Titanium Substrates.” MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS 119. https://doi.org/10.1016/j.msec.2020.111513.
Chicago author-date (all authors)
Thukkaram, Monica, Mykhailo Vaidulych, Ondrej Kylian, Petra Rigole, Sheida Aliakbarshirazi, Mahtab Asadian, Anton Nikiforov, Hynek Biederman, Tom Coenye, Gijs Du Laing, Rino Morent, Alexander Van Tongel, Lieven De Wilde, Kim Verbeken, and Nathalie De Geyter. 2021. “Biological Activity and Antimicrobial Property of Cu/a-C:H Nanocomposites and Nanolayered Coatings on Titanium Substrates.” MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS 119. doi:10.1016/j.msec.2020.111513.
Vancouver
1.
Thukkaram M, Vaidulych M, Kylian O, Rigole P, Aliakbarshirazi S, Asadian M, et al. Biological activity and antimicrobial property of Cu/a-C:H nanocomposites and nanolayered coatings on titanium substrates. MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS. 2021;119.
IEEE
[1]
M. Thukkaram et al., “Biological activity and antimicrobial property of Cu/a-C:H nanocomposites and nanolayered coatings on titanium substrates,” MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS, vol. 119, 2021.
@article{8690811,
  abstract     = {{Infection associated with titanium based implants remains the most serious problem in implant surgery hence it is important to find optimal strategies to prevent infections. In the present study, we investigated the surface properties, antibacterial activity and biocompatibility of nanocomposite coatings based on an amorphous hydrocarbon (a-C:H) film containing copper nanoparticles (CuNPs) deposited on Ti discs via a gas aggregation cluster source. Three different Cu/a-C:H coatings with approximately the same amount of embedded CuNPs with and without barrier a-C:H layer were fabricated. The obtained results revealed that different structures of the produced coatings have significantly different release rates of Cu ions from the coatings into the aqueous media. This subsequently influences the antibacterial efficiency and osteoblast cell viability of the treated coatings. Coatings with the highest number of CuNPs resulted in excellent antibacterial activity exhibiting approximately 4 log reduction of E.coli and S.aureus after 24 h incubation. The cytotoxicity study revealed that after 7 day cell seeding, even the coating with the highest Cu at.% (4 at.%) showed a cell viability of.90%. Consequently, the coating, formed with a properly tailored number of CuNPs and a-C:H barrier thickness offer a strong antibacterial effect without any harm to osteoblast cells.}},
  articleno    = {{111513}},
  author       = {{Thukkaram, Monica and Vaidulych, Mykhailo and Kylian, Ondrej and Rigole, Petra and Aliakbarshirazi, Sheida and Asadian, Mahtab and Nikiforov, Anton and Biederman, Hynek and Coenye, Tom and Du Laing, Gijs and Morent, Rino and Van Tongel, Alexander and De Wilde, Lieven and Verbeken, Kim and De Geyter, Nathalie}},
  issn         = {{0928-4931}},
  journal      = {{MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS}},
  keywords     = {{Antibacterial coatings,Implant infection,Biocompatibility,Ti implants,Controlled release,CARBON DLC COATINGS,COPPER NANOPARTICLES,TIO2 COATINGS,IN-VITRO,ANTIBACTERIAL,CYTOTOXICITY,OSTEOBLASTS,SCAFFOLDS,FILMS}},
  language     = {{eng}},
  pages        = {{12}},
  title        = {{Biological activity and antimicrobial property of Cu/a-C:H nanocomposites and nanolayered coatings on titanium substrates}},
  url          = {{http://dx.doi.org/10.1016/j.msec.2020.111513}},
  volume       = {{119}},
  year         = {{2021}},
}

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