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Cov-MS : a community-based template assay for clinical MS-based protein detection in SARS-CoV-2 patients

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Organization
Abstract
Rising population density and global mobility are among the reasons why pathogens such as SARS-CoV-2, the virus that causes COVID-19, spread so rapidly across the globe. The policy response to such pandemics will always have to include accurate monitoring of the spread, as this provides one of the few alternatives to total lockdown. However, COVID-19 diagnosis is currently performed almost exclusively by Reverse Transcription Polymerase Chain Reaction (RT-PCR). Although this is efficient, automatable and acceptably cheap, reliance on one type of technology comes with serious caveats, as illustrated by recurring reagent and test shortages. We therefore developed an alternative diagnostic test that detects proteolytically digested SARS-CoV-2 proteins using Mass Spectrometry (MS). We established the Cov-MS consortium, consisting of fifteen academic labs and several industrial partners to increase applicability, accessibility, sensitivity and robustness of this kind of SARS-CoV-2 detection. This in turn gave rise to the Cov-MS Digital Incubator that allows other labs to join the effort, navigate and share their optimizations, and translate the assay into their clinic. As this test relies on viral proteins instead of RNA, it provides an orthogonal and complementary approach to RT-PCR, using other reagents that are relatively inexpensive and widely available, as well as orthogonally skilled personnel and different instruments. Data are available via ProteomeXchange with identifier PXD022550.

Citation

Please use this url to cite or link to this publication:

MLA
Van Puyvelde, Bart, et al. “Cov-MS : A Community-Based Template Assay for Clinical MS-Based Protein Detection in SARS-CoV-2 Patients.” MedRxiv, 2020, doi:10.1101/2020.11.18.20231688.
APA
Van Puyvelde, B., Van Uytfanghe, K., Tytgat, O., Van Oudenhove, L., Gabriels, R., Bouwmeester, R., … Dhaenens, M. (2020). Cov-MS : a community-based template assay for clinical MS-based protein detection in SARS-CoV-2 patients. https://doi.org/10.1101/2020.11.18.20231688
Chicago author-date
Van Puyvelde, Bart, Katleen Van Uytfanghe, Olivier Tytgat, L. Van Oudenhove, Ralf Gabriels, Robbin Bouwmeester, Simon Daled, et al. 2020. “Cov-MS : A Community-Based Template Assay for Clinical MS-Based Protein Detection in SARS-CoV-2 Patients.” MedRxiv. https://doi.org/10.1101/2020.11.18.20231688.
Chicago author-date (all authors)
Van Puyvelde, Bart, Katleen Van Uytfanghe, Olivier Tytgat, L. Van Oudenhove, Ralf Gabriels, Robbin Bouwmeester, Simon Daled, Tim Van Den Bossche, Pathmanaban Ramasamy, Sigrid Verhelst, Laura De Clerck, Laura Corveleyn, Nathan Debunne, Evelien Wynendaele, Bart De Spiegeleer, Péter Judák, Kris Roels, Laurie De Wilde, Peter Van Eenoo, Tim Reyns, Marc Cherlet, E. Dumont, G. Debyser, R. t’Kindt, K. Sandra, Surya Gupta, Nicolas Drouin, Amy Harms, Thomas Hankemeier, DJL Jones, P. Gupta, D. Lane, C.S. Lane, S. El Ouadi, JB. Vincendet, N. Morrice, S. Oehrle, N. Tanna, S. Silvester, S. Hannam, F. Sigloch, A. Bhangu-Uhlmann, J. Claereboudt, L. Anderson, M. Razavi, Sven Degroeve, L. Cuypers, Christophe Stove, K. Lagrou, G. Martens, Dieter Deforce, Lennart Martens, J.P.C. Vissers, and Maarten Dhaenens. 2020. “Cov-MS : A Community-Based Template Assay for Clinical MS-Based Protein Detection in SARS-CoV-2 Patients.” MedRxiv. doi:10.1101/2020.11.18.20231688.
Vancouver
1.
Van Puyvelde B, Van Uytfanghe K, Tytgat O, Van Oudenhove L, Gabriels R, Bouwmeester R, et al. Cov-MS : a community-based template assay for clinical MS-based protein detection in SARS-CoV-2 patients. medRxiv. 2020.
IEEE
[1]
B. Van Puyvelde et al., “Cov-MS : a community-based template assay for clinical MS-based protein detection in SARS-CoV-2 patients,” medRxiv. 2020.
@misc{8690766,
  abstract     = {{Rising population density and global mobility are among the reasons why pathogens such as SARS-CoV-2, the virus that causes COVID-19, spread so rapidly across the globe. The policy response to such pandemics will always have to include accurate monitoring of the spread, as this provides one of the few alternatives to total lockdown. However, COVID-19 diagnosis is currently performed almost exclusively by Reverse Transcription Polymerase Chain Reaction (RT-PCR). Although this is efficient, automatable and acceptably cheap, reliance on one type of technology comes with serious caveats, as illustrated by recurring reagent and test shortages. We therefore developed an alternative diagnostic test that detects proteolytically digested SARS-CoV-2 proteins using Mass Spectrometry (MS). We established the Cov-MS consortium, consisting of fifteen academic labs and several industrial partners to increase applicability, accessibility, sensitivity and robustness of this kind of SARS-CoV-2 detection. This in turn gave rise to the Cov-MS Digital Incubator that allows other labs to join the effort, navigate and share their optimizations, and translate the assay into their clinic. As this test relies on viral proteins instead of RNA, it provides an orthogonal and complementary approach to RT-PCR, using other reagents that are relatively inexpensive and widely available, as well as orthogonally skilled personnel and different instruments. Data are available via ProteomeXchange with identifier PXD022550.}},
  author       = {{Van Puyvelde, Bart and Van Uytfanghe, Katleen and Tytgat, Olivier and Van Oudenhove, L. and Gabriels, Ralf and Bouwmeester, Robbin and Daled, Simon and Van Den Bossche, Tim and Ramasamy, Pathmanaban and Verhelst, Sigrid and De Clerck, Laura and Corveleyn, Laura and Debunne, Nathan and Wynendaele, Evelien and De Spiegeleer, Bart and Judák, Péter and Roels, Kris and De Wilde, Laurie and Van Eenoo, Peter and Reyns, Tim and Cherlet, Marc and Dumont, E. and Debyser, G. and t’Kindt, R. and Sandra, K. and Gupta, Surya and Drouin, Nicolas and Harms, Amy and Hankemeier, Thomas and Jones, DJL and Gupta, P. and Lane, D. and Lane, C.S. and El Ouadi, S. and Vincendet, JB. and Morrice, N. and Oehrle, S. and Tanna, N. and Silvester, S. and Hannam, S. and Sigloch, F. and Bhangu-Uhlmann, A. and Claereboudt, J. and Anderson, L. and Razavi, M. and Degroeve, Sven and Cuypers, L. and Stove, Christophe and Lagrou, K. and Martens, G. and Deforce, Dieter and Martens, Lennart and Vissers, J.P.C. and Dhaenens, Maarten}},
  language     = {{eng}},
  series       = {{medRxiv}},
  title        = {{Cov-MS : a community-based template assay for clinical MS-based protein detection in SARS-CoV-2 patients}},
  url          = {{http://dx.doi.org/10.1101/2020.11.18.20231688}},
  year         = {{2020}},
}

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