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Investigation of Ag/a-C:H nanocomposite coatings on titanium for orthopedic applications

Monica Thukkaram (UGent) , Mykhailo Vaidulych, Ondrej Kylian, Jan Hanus, Petra Rigole (UGent) , Sheida Aliakbarshirazi (UGent) , Mahtab Asadian (UGent) , Anton Nikiforov (UGent) , Alexander Van Tongel (UGent) , Hynek Biederman, et al.
(2020) ACS APPLIED MATERIALS & INTERFACES. 12(21). p.23655-23666
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Abstract
One of the leading causes of failure for any bone implant is implant-associated infections. The implant-bone interface is in fact the crucial site of infection where both the microorganisms and cells compete to populate the newly introduced implant surface. Most of the work dealing with this issue has focused on the design of implant coatings capable of preventing infection while ignoring cell proliferation or vice versa. The present study is therefore focused on investigating the antibacterial and biological properties of nanocomposite coatings based on an amorphous hydrocarbon (a-C:H) matrix containing silver nanoparticles (AgNPs). a-C:H coatings with varying silver concentrations were generated directly on medical grade titanium substrates using a combination of a gas aggregation source (GAS) and a plasma-enhanced chemical vapor deposition (PE-CVD) process. The obtained results revealed that the surface silver content increased from 1.3 at % to 5.3 at % by increasing the used DC magnetron current in the GAS from 200 to 500 mA. The in vitro antibacterial assays revealed that the nanocomposites with the highest number of silver content exhibited excellent antibacterial activities resulting in a 6-log reduction of Escherichia coli and a 4-log reduction of Staphylococcus aureus after 24 h of incubation. An MTT assay, fluorescence live/dead staining, and SEM microscopy observations of MC3T3 cells seeded on the uncoated and coated Ti substrates also showed that increasing the amount of AgNPs in the nanocomposites had no notable impact on their cytocompatibility, while improved cell proliferation was especially observed for the nanocomposites possessing a low amount of AgNPs. These controllable Ag/a-C:H nanocomposites on Ti substrates, which simultaneously provide an excellent antibacterial performance and good biocompatibility, could thus have promising applications in orthopedics and other biomedical implants.
Keywords
SILVER NANOPARTICLES, NANOCLUSTER FILMS, TRACT-INFECTION, CELL, VIABILITY, CARBON-FILMS, IN-VITRO, DEPOSITION, BACTERIAL, CLUSTER, EFFICACY, Ag nanocomposite coatings, GAS, PE-CVD, antibacterial efficacy, biocompatibility, Ti implants

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MLA
Thukkaram, Monica, et al. “Investigation of Ag/a-C:H Nanocomposite Coatings on Titanium for Orthopedic Applications.” ACS APPLIED MATERIALS & INTERFACES, vol. 12, no. 21, 2020, pp. 23655–66, doi:10.1021/acsami.9b23237.
APA
Thukkaram, M., Vaidulych, M., Kylian, O., Hanus, J., Rigole, P., Aliakbarshirazi, S., … De Geyter, N. (2020). Investigation of Ag/a-C:H nanocomposite coatings on titanium for orthopedic applications. ACS APPLIED MATERIALS & INTERFACES, 12(21), 23655–23666. https://doi.org/10.1021/acsami.9b23237
Chicago author-date
Thukkaram, Monica, Mykhailo Vaidulych, Ondrej Kylian, Jan Hanus, Petra Rigole, Sheida Aliakbarshirazi, Mahtab Asadian, et al. 2020. “Investigation of Ag/a-C:H Nanocomposite Coatings on Titanium for Orthopedic Applications.” ACS APPLIED MATERIALS & INTERFACES 12 (21): 23655–66. https://doi.org/10.1021/acsami.9b23237.
Chicago author-date (all authors)
Thukkaram, Monica, Mykhailo Vaidulych, Ondrej Kylian, Jan Hanus, Petra Rigole, Sheida Aliakbarshirazi, Mahtab Asadian, Anton Nikiforov, Alexander Van Tongel, Hynek Biederman, Tom Coenye, Gijs Du Laing, Rino Morent, Lieven De Wilde, Kim Verbeken, and Nathalie De Geyter. 2020. “Investigation of Ag/a-C:H Nanocomposite Coatings on Titanium for Orthopedic Applications.” ACS APPLIED MATERIALS & INTERFACES 12 (21): 23655–23666. doi:10.1021/acsami.9b23237.
Vancouver
1.
Thukkaram M, Vaidulych M, Kylian O, Hanus J, Rigole P, Aliakbarshirazi S, et al. Investigation of Ag/a-C:H nanocomposite coatings on titanium for orthopedic applications. ACS APPLIED MATERIALS & INTERFACES. 2020;12(21):23655–66.
IEEE
[1]
M. Thukkaram et al., “Investigation of Ag/a-C:H nanocomposite coatings on titanium for orthopedic applications,” ACS APPLIED MATERIALS & INTERFACES, vol. 12, no. 21, pp. 23655–23666, 2020.
@article{8690549,
  abstract     = {One of the leading causes of failure for any bone implant is implant-associated infections. The implant-bone interface is in fact the crucial site of infection where both the microorganisms and cells compete to populate the newly introduced implant surface. Most of the work dealing with this issue has focused on the design of implant coatings capable of preventing infection while ignoring cell proliferation or vice versa. The present study is therefore focused on investigating the antibacterial and biological properties of nanocomposite coatings based on an amorphous hydrocarbon (a-C:H) matrix containing silver nanoparticles (AgNPs). a-C:H coatings with varying silver concentrations were generated directly on medical grade titanium substrates using a combination of a gas aggregation source (GAS) and a plasma-enhanced chemical vapor deposition (PE-CVD) process. The obtained results revealed that the surface silver content increased from 1.3 at % to 5.3 at % by increasing the used DC magnetron current in the GAS from 200 to 500 mA. The in vitro antibacterial assays revealed that the nanocomposites with the highest number of silver content exhibited excellent antibacterial activities resulting in a 6-log reduction of Escherichia coli and a 4-log reduction of Staphylococcus aureus after 24 h of incubation. An MTT assay, fluorescence live/dead staining, and SEM microscopy observations of MC3T3 cells seeded on the uncoated and coated Ti substrates also showed that increasing the amount of AgNPs in the nanocomposites had no notable impact on their cytocompatibility, while improved cell proliferation was especially observed for the nanocomposites possessing a low amount of AgNPs. These controllable Ag/a-C:H nanocomposites on Ti substrates, which simultaneously provide an excellent antibacterial performance and good biocompatibility, could thus have promising applications in orthopedics and other biomedical implants.},
  author       = {Thukkaram, Monica and Vaidulych, Mykhailo and Kylian, Ondrej and Hanus, Jan and Rigole, Petra and Aliakbarshirazi, Sheida and Asadian, Mahtab and Nikiforov, Anton and Van Tongel, Alexander and Biederman, Hynek and Coenye, Tom and Du Laing, Gijs and Morent, Rino and De Wilde, Lieven and Verbeken, Kim and De Geyter, Nathalie},
  issn         = {1944-8244},
  journal      = {ACS APPLIED MATERIALS & INTERFACES},
  keywords     = {SILVER NANOPARTICLES,NANOCLUSTER FILMS,TRACT-INFECTION,CELL,VIABILITY,CARBON-FILMS,IN-VITRO,DEPOSITION,BACTERIAL,CLUSTER,EFFICACY,Ag nanocomposite coatings,GAS,PE-CVD,antibacterial efficacy,biocompatibility,Ti implants},
  language     = {eng},
  number       = {21},
  pages        = {23655--23666},
  title        = {Investigation of Ag/a-C:H nanocomposite coatings on titanium for orthopedic applications},
  url          = {http://dx.doi.org/10.1021/acsami.9b23237},
  volume       = {12},
  year         = {2020},
}
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