Advanced search
1 file | 3.53 MB Add to list

Diagnosing COVID-19 : towards a feasible COVID-19 rule-out protocol

Jozefien De Clercq (UGent) , Thomas Malfait (UGent) , Simon Malfait (UGent) , Jerina Boelens (UGent) , Liselotte Coorevits, Elizaveta Padalko (UGent) , Stien Vandendriessche (UGent) , Bruno Verhasselt (UGent) , Lieve Morbée (UGent) , Fre Bauters (UGent) , et al.
Author
Organization
Abstract
Introduction: We present the results of the COVID-19 rule-out protocol at Ghent University Hospital, a step-wise testing approach which included repeat NFS SARS-CoV-2 rRT-PCR, respiratory multiplex RT-PCR, low-dose chest CT and bronchoscopy with BAL to confirm or rule-out SARS-CoV-2 infection in patients admitted with symptoms suggestive of COVID-19. Results: Between 19 March 2020 and 30 April 2020, 455 non-critically ill patients with symptoms suspect for COVID-19 were admitted. The initial NFS for SARS-CoV-2 rRT-PCR yielded 66.9%, the second NFS 25.4% and bronchoscopy with BAL 5.9% of total COVID-19 diagnoses. In the BAL fluid, other respiratory pathogens were detected in 65% (13/20) of the COVID-19 negative patients and only in 1/7 COVID-19 positive patients. Retrospective antibody testing at the time around BAL sampling showed a positive IgA or IgG in 42.9 % of the COVID-19 positive and 10.5% of the COVID-19 negative group. Follow-up serology showed 100% COVID-19 positivity in the COVID-19 positive group and 100% IgG negativity in the COVID-19 negative group. Conclusion: In our experience, bronchoscopy with BAL can have an added value to rule-in or rule-out COVID-19 in patients with clinical and radiographical high-likelihood of COVID-19 and repeated negative NFS testing. Furthermore, culture and respiratory multiplex PCR on BAL fluid can aid to identify alternative microbial etiological agents in this group. Retrospective analysis of antibody development in this selected group of patients suggests that the implementation of serological assays in the routine testing protocol will decrease the need for invasive procedures like bronchoscopy.
Keywords
COVID-19, SARS-CoV-2, rRT-PCR, diagnostics, bronchoalveolar lavage

Downloads

  • (...).pdf
    • full text (Published version)
    • |
    • UGent only
    • |
    • PDF
    • |
    • 3.53 MB

Citation

Please use this url to cite or link to this publication:

MLA
De Clercq, Jozefien, et al. “Diagnosing COVID-19 : Towards a Feasible COVID-19 Rule-out Protocol.” ACTA CLINICA BELGICA, 2021, doi:10.1080/17843286.2021.1883362.
APA
De Clercq, J., Malfait, T., Malfait, S., Boelens, J., Coorevits, L., Padalko, E., … Van Braeckel, E. (2021). Diagnosing COVID-19 : towards a feasible COVID-19 rule-out protocol. ACTA CLINICA BELGICA. https://doi.org/10.1080/17843286.2021.1883362
Chicago author-date
De Clercq, Jozefien, Thomas Malfait, Simon Malfait, Jerina Boelens, Liselotte Coorevits, Elizaveta Padalko, Stien Vandendriessche, et al. 2021. “Diagnosing COVID-19 : Towards a Feasible COVID-19 Rule-out Protocol.” ACTA CLINICA BELGICA. https://doi.org/10.1080/17843286.2021.1883362.
Chicago author-date (all authors)
De Clercq, Jozefien, Thomas Malfait, Simon Malfait, Jerina Boelens, Liselotte Coorevits, Elizaveta Padalko, Stien Vandendriessche, Bruno Verhasselt, Lieve Morbée, Fré Bauters, Katrien Hertegonne, Dieter Stevens, Yannick Vande Weygaerde, Karim Vermaelen, Wim Van Biesen, Floris Vanommeslaeghe, Francis Verbeke, Ruth Piers, Nele Van Den Noortgate, Tania Desmet, Frank Vermassen, Linos Vandekerckhove, and Eva Van Braeckel. 2021. “Diagnosing COVID-19 : Towards a Feasible COVID-19 Rule-out Protocol.” ACTA CLINICA BELGICA. doi:10.1080/17843286.2021.1883362.
Vancouver
1.
De Clercq J, Malfait T, Malfait S, Boelens J, Coorevits L, Padalko E, et al. Diagnosing COVID-19 : towards a feasible COVID-19 rule-out protocol. ACTA CLINICA BELGICA. 2021;
IEEE
[1]
J. De Clercq et al., “Diagnosing COVID-19 : towards a feasible COVID-19 rule-out protocol,” ACTA CLINICA BELGICA, 2021.
@article{8690127,
  abstract     = {{Introduction: We present the results of the COVID-19 rule-out protocol at Ghent University Hospital, a step-wise testing approach which included repeat NFS SARS-CoV-2 rRT-PCR, respiratory multiplex RT-PCR, low-dose chest CT and bronchoscopy with BAL to confirm or rule-out SARS-CoV-2 infection in patients admitted with symptoms suggestive of COVID-19.

Results: Between 19 March 2020 and 30 April 2020, 455 non-critically ill patients with symptoms suspect for COVID-19 were admitted. The initial NFS for SARS-CoV-2 rRT-PCR yielded 66.9%, the second NFS 25.4% and bronchoscopy with BAL 5.9% of total COVID-19 diagnoses. In the BAL fluid, other respiratory pathogens were detected in 65% (13/20) of the COVID-19 negative patients and only in 1/7 COVID-19 positive patients. Retrospective antibody testing at the time around BAL sampling showed a positive IgA or IgG in 42.9 % of the COVID-19 positive and 10.5% of the COVID-19 negative group. Follow-up serology showed 100% COVID-19 positivity in the COVID-19 positive group and 100% IgG negativity in the COVID-19 negative group.

Conclusion: In our experience, bronchoscopy with BAL can have an added value to rule-in or rule-out COVID-19 in patients with clinical and radiographical high-likelihood of COVID-19 and repeated negative NFS testing. Furthermore, culture and respiratory multiplex PCR on BAL fluid can aid to identify alternative microbial etiological agents in this group. Retrospective analysis of antibody development in this selected group of patients suggests that the implementation of serological assays in the routine testing protocol will decrease the need for invasive procedures like bronchoscopy.}},
  author       = {{De Clercq, Jozefien and Malfait, Thomas and Malfait, Simon and Boelens, Jerina and Coorevits, Liselotte and Padalko, Elizaveta and Vandendriessche, Stien and Verhasselt, Bruno and Morbée, Lieve and Bauters, Fre and Hertegonne, Katrien and Stevens, Dieter and Vande Weygaerde, Yannick and Vermaelen, Karim and Van Biesen, Wim and Vanommeslaeghe, Floris and Verbeke, Francis and Piers, Ruth and Van Den Noortgate, Nele and Desmet, Tania and Vermassen, Frank and Vandekerckhove, Linos and Van Braeckel, Eva}},
  issn         = {{1784-3286}},
  journal      = {{ACTA CLINICA BELGICA}},
  keywords     = {{COVID-19,SARS-CoV-2,rRT-PCR,diagnostics,bronchoalveolar lavage}},
  language     = {{eng}},
  pages        = {{9}},
  title        = {{Diagnosing COVID-19 : towards a feasible COVID-19 rule-out protocol}},
  url          = {{http://dx.doi.org/10.1080/17843286.2021.1883362}},
  year         = {{2021}},
}

Altmetric
View in Altmetric
Web of Science
Times cited: