Advanced search
1 file | 763.66 KB Add to list

Practical methods to assess the effects of heat stress on the quality of frozen-thawed Belgian Blue semen in field conditions

Author
Organization
Abstract
The increased exportation of semen and embryos of double-muscled beef breeds to tropical and developing countries makes it important to investigate the reproductive capacity of these breeds in adapting to tropical conditions. The aim of this study was to evaluate the quality of Belgian Blue semen collected after there is heat-stress (HS; as a mimic of tropical condition) compared with non-heat stressed (NHS; as their comfort zone), using practical spermatozoa staining methods such that prevail in developing countries. There was screening of semen kinetics using CASA and evaluation of their DNA-, acrosome, plasma membrane-integrity, and mitochondrial activity. For each staining technique, there was evaluation of 12 frozen-thawed semen samples from six Belgian Blue bulls collected after there were HS and NHS conditions in Belgium. Mixed linear regression models were used to assess the effects of HS for each CASA variable and staining method outcome using the replicate nested with bull as a random effect. There were differences (P < 0.05) in values when there were semen collections following HS and NHS conditions for several post-thawing kinetic variables. Furthermore, the mean percentages of DNA-, acrosome-, and plasma membrane-integrity, as well as mitochondrial activity were greater (P < 0.05) when semen was collected following NHS compared with HS conditions. Conclusively, results indicated that when there was collection of semen following HS conditions, there were detrimental effects on the viability and quality of Belgian Blue semen which is an important consideration for the semen collection, processing, and evaluation in tropical countries.
Keywords
SPERM DNA FRAGMENTATION, OXIDATIVE STRESS, FERTILITY, MOTILITY, GLUTATHIONE, GENERATION, VIABILITY, CLIMATE, STORAGE, TRAITS, Double-muscled bull, Sperm, DNA integrity, Acrosome integrity, Membrane, integrity, Mitochondrial activity

Downloads

  • (...).pdf
    • full text (Published version)
    • |
    • UGent only
    • |
    • PDF
    • |
    • 763.66 KB

Citation

Please use this url to cite or link to this publication:

MLA
Gretania Residiwati, et al. “Practical Methods to Assess the Effects of Heat Stress on the Quality of Frozen-Thawed Belgian Blue Semen in Field Conditions.” ANIMAL REPRODUCTION SCIENCE, vol. 221, 2020, doi:10.1016/j.anireprosci.2020.106572.
APA
Gretania Residiwati, Tuska, H. S. A., Budiono, B., Vechiato Kawai, G. K., Seifi-Jamadi, A., Santoro, D., … Van Soom, A. (2020). Practical methods to assess the effects of heat stress on the quality of frozen-thawed Belgian Blue semen in field conditions. ANIMAL REPRODUCTION SCIENCE, 221. https://doi.org/10.1016/j.anireprosci.2020.106572
Chicago author-date
Gretania Residiwati, Habib Syaiful Arif Tuska, Budiono Budiono, Giulia Kiyomi Vechiato Kawai, Afshin Seifi-Jamadi, Davide Santoro, Bart Leemans, et al. 2020. “Practical Methods to Assess the Effects of Heat Stress on the Quality of Frozen-Thawed Belgian Blue Semen in Field Conditions.” ANIMAL REPRODUCTION SCIENCE 221. https://doi.org/10.1016/j.anireprosci.2020.106572.
Chicago author-date (all authors)
Gretania Residiwati, Habib Syaiful Arif Tuska, Budiono Budiono, Giulia Kiyomi Vechiato Kawai, Afshin Seifi-Jamadi, Davide Santoro, Bart Leemans, Christophe Boccart, Osvaldo Américo Bogado Pascottini, Geert Opsomer, and Ann Van Soom. 2020. “Practical Methods to Assess the Effects of Heat Stress on the Quality of Frozen-Thawed Belgian Blue Semen in Field Conditions.” ANIMAL REPRODUCTION SCIENCE 221. doi:10.1016/j.anireprosci.2020.106572.
Vancouver
1.
Gretania Residiwati, Tuska HSA, Budiono B, Vechiato Kawai GK, Seifi-Jamadi A, Santoro D, et al. Practical methods to assess the effects of heat stress on the quality of frozen-thawed Belgian Blue semen in field conditions. ANIMAL REPRODUCTION SCIENCE. 2020;221.
IEEE
[1]
Gretania Residiwati et al., “Practical methods to assess the effects of heat stress on the quality of frozen-thawed Belgian Blue semen in field conditions,” ANIMAL REPRODUCTION SCIENCE, vol. 221, 2020.
@article{8689205,
  abstract     = {{The increased exportation of semen and embryos of double-muscled beef breeds to tropical and developing countries makes it important to investigate the reproductive capacity of these breeds in adapting to tropical conditions. The aim of this study was to evaluate the quality of Belgian Blue semen collected after there is heat-stress (HS; as a mimic of tropical condition) compared with non-heat stressed (NHS; as their comfort zone), using practical spermatozoa staining methods such that prevail in developing countries. There was screening of semen kinetics using CASA and evaluation of their DNA-, acrosome, plasma membrane-integrity, and mitochondrial activity. For each staining technique, there was evaluation of 12 frozen-thawed semen samples from six Belgian Blue bulls collected after there were HS and NHS conditions in Belgium. Mixed linear regression models were used to assess the effects of HS for each CASA variable and staining method outcome using the replicate nested with bull as a random effect. There were differences (P < 0.05) in values when there were semen collections following HS and NHS conditions for several post-thawing kinetic variables. Furthermore, the mean percentages of DNA-, acrosome-, and plasma membrane-integrity, as well as mitochondrial activity were greater (P < 0.05) when semen was collected following NHS compared with HS conditions. Conclusively, results indicated that when there was collection of semen following HS conditions, there were detrimental effects on the viability and quality of Belgian Blue semen which is an important consideration for the semen collection, processing, and evaluation in tropical countries.}},
  articleno    = {{106572}},
  author       = {{Gretania Residiwati and Tuska, Habib Syaiful Arif and Budiono, Budiono and Vechiato Kawai, Giulia Kiyomi and Seifi-Jamadi, Afshin and Santoro, Davide and Leemans, Bart and Boccart, Christophe and Bogado Pascottini, Osvaldo Américo and Opsomer, Geert and Van Soom, Ann}},
  issn         = {{0378-4320}},
  journal      = {{ANIMAL REPRODUCTION SCIENCE}},
  keywords     = {{SPERM DNA FRAGMENTATION,OXIDATIVE STRESS,FERTILITY,MOTILITY,GLUTATHIONE,GENERATION,VIABILITY,CLIMATE,STORAGE,TRAITS,Double-muscled bull,Sperm,DNA integrity,Acrosome integrity,Membrane,integrity,Mitochondrial activity}},
  language     = {{eng}},
  pages        = {{7}},
  title        = {{Practical methods to assess the effects of heat stress on the quality of frozen-thawed Belgian Blue semen in field conditions}},
  url          = {{http://dx.doi.org/10.1016/j.anireprosci.2020.106572}},
  volume       = {{221}},
  year         = {{2020}},
}

Altmetric
View in Altmetric
Web of Science
Times cited: