Advanced search
1 file | 1.53 MB Add to list

Molecular study of drought response in the Mediterranean conifer Pinus Pinaster Ait. : differential transcriptomic profiling reveals constitutive water deficit‐independent drought tolerance mechanisms

(2020) ECOLOGY AND EVOLUTION. 10(18). p.9788-9807
Author
Organization
Abstract
Adaptation of long‐living forest trees to respond to environmental changes is essential to secure their performance under adverse conditions. Water deficit is one of the most significant stress factors determining tree growth and survival. Maritime pine (Pinus pinaster Ait.), the main source of softwood in southwestern Europe, is subjected to recurrent drought periods which, according to climate change predictions for the years to come, will progressively increase in the Mediterranean region. The mechanisms regulating pine adaptive responses to environment are still largely unknown. The aim of this work was to go a step further in understanding the molecular mechanisms underlying maritime pine response to water stress and drought tolerance at the whole plant level. A global transcriptomic profiling of roots, stems, and needles was conducted to analyze the performance of siblings showing contrasted responses to water deficit from an ad hoc designed full‐sib family. Although P. pinaster is considered a recalcitrant species for vegetative propagation in adult phase, the analysis was conducted using vegetatively propagated trees exposed to two treatments: well‐watered and moderate water stress. The comparative analyses led us to identify organ‐specific genes, constitutively expressed as well as differentially expressed when comparing control versus water stress conditions, in drought‐sensitive and drought‐tolerant genotypes. Different response strategies can point out, with tolerant individuals being pre‐adapted for coping with drought by constitutively expressing stress‐related genes that are detected only in latter stages on sensitive individuals subjected to drought.
Keywords
Ecology, Ecology, Evolution, Behavior and Systematics, Nature and Landscape Conservation, differential transcript profiles, Mediterranean conifer, pre-adapted genotypes, response strategies, water stress, PINE PINUS-PINASTER, MARITIME PINE, ABIOTIC-STRESS, GENE FAMILY, OSMOTIC ADJUSTMENT, USE EFFICIENCY, CLIMATE-CHANGE, GAS-EXCHANGE, AIT., IDENTIFICATION

Downloads

  • de Maria et al. 2020 Ecology and Evolution 10 9788.pdf
    • full text (Published version)
    • |
    • open access
    • |
    • PDF
    • |
    • 1.53 MB

Citation

Please use this url to cite or link to this publication:

MLA
de María, Nuria, et al. “Molecular Study of Drought Response in the Mediterranean Conifer Pinus Pinaster Ait. : Differential Transcriptomic Profiling Reveals Constitutive Water Deficit‐independent Drought Tolerance Mechanisms.” ECOLOGY AND EVOLUTION, vol. 10, no. 18, 2020, pp. 9788–807, doi:10.1002/ece3.6613.
APA
de María, N., Guevara, M. Á., Perdiguero, P., Vélez, M. D., Cabezas, J. A., López‐Hinojosa, M., … Cervera, M. T. (2020). Molecular study of drought response in the Mediterranean conifer Pinus Pinaster Ait. : differential transcriptomic profiling reveals constitutive water deficit‐independent drought tolerance mechanisms. ECOLOGY AND EVOLUTION, 10(18), 9788–9807. https://doi.org/10.1002/ece3.6613
Chicago author-date
María, Nuria de, María Ángeles Guevara, Pedro Perdiguero, María Dolores Vélez, José Antonio Cabezas, Miriam López‐Hinojosa, Zhen Li, et al. 2020. “Molecular Study of Drought Response in the Mediterranean Conifer Pinus Pinaster Ait. : Differential Transcriptomic Profiling Reveals Constitutive Water Deficit‐independent Drought Tolerance Mechanisms.” ECOLOGY AND EVOLUTION 10 (18): 9788–9807. https://doi.org/10.1002/ece3.6613.
Chicago author-date (all authors)
de María, Nuria, María Ángeles Guevara, Pedro Perdiguero, María Dolores Vélez, José Antonio Cabezas, Miriam López‐Hinojosa, Zhen Li, Luís Manuel Díaz, Alberto Pizarro, José Antonio Mancha, Lieven Sterck, David Sánchez‐Gómez, Célia Miguel, Carmen Collada, María Carmen Díaz‐Sala, and María Teresa Cervera. 2020. “Molecular Study of Drought Response in the Mediterranean Conifer Pinus Pinaster Ait. : Differential Transcriptomic Profiling Reveals Constitutive Water Deficit‐independent Drought Tolerance Mechanisms.” ECOLOGY AND EVOLUTION 10 (18): 9788–9807. doi:10.1002/ece3.6613.
Vancouver
1.
de María N, Guevara MÁ, Perdiguero P, Vélez MD, Cabezas JA, López‐Hinojosa M, et al. Molecular study of drought response in the Mediterranean conifer Pinus Pinaster Ait. : differential transcriptomic profiling reveals constitutive water deficit‐independent drought tolerance mechanisms. ECOLOGY AND EVOLUTION. 2020;10(18):9788–807.
IEEE
[1]
N. de María et al., “Molecular study of drought response in the Mediterranean conifer Pinus Pinaster Ait. : differential transcriptomic profiling reveals constitutive water deficit‐independent drought tolerance mechanisms,” ECOLOGY AND EVOLUTION, vol. 10, no. 18, pp. 9788–9807, 2020.
@article{8688445,
  abstract     = {Adaptation of long‐living forest trees to respond to environmental changes is essential to secure their performance under adverse conditions. Water deficit is one of the most significant stress factors determining tree growth and survival. Maritime pine (Pinus pinaster Ait.), the main source of softwood in southwestern Europe, is subjected to recurrent drought periods which, according to climate change predictions for the years to come, will progressively increase in the Mediterranean region. The mechanisms regulating pine adaptive responses to environment are still largely unknown. The aim of this work was to go a step further in understanding the molecular mechanisms underlying maritime pine response to water stress and drought tolerance at the whole plant level. A global transcriptomic profiling of roots, stems, and needles was conducted to analyze the performance of siblings showing contrasted responses to water deficit from an ad hoc designed full‐sib family. Although P. pinaster is considered a recalcitrant species for vegetative propagation in adult phase, the analysis was conducted using vegetatively propagated trees exposed to two treatments: well‐watered and moderate water stress. The comparative analyses led us to identify organ‐specific genes, constitutively expressed as well as differentially expressed when comparing control versus water stress conditions, in drought‐sensitive and drought‐tolerant genotypes. Different response strategies can point out, with tolerant individuals being pre‐adapted for coping with drought by constitutively expressing stress‐related genes that are detected only in latter stages on sensitive individuals subjected to drought.},
  author       = {de María, Nuria and Guevara, María Ángeles and Perdiguero, Pedro and Vélez, María Dolores and Cabezas, José Antonio and López‐Hinojosa, Miriam and Li, Zhen and Díaz, Luís Manuel and Pizarro, Alberto and Mancha, José Antonio and Sterck, Lieven and Sánchez‐Gómez, David and Miguel, Célia and Collada, Carmen and Díaz‐Sala, María Carmen and Cervera, María Teresa},
  issn         = {2045-7758},
  journal      = {ECOLOGY AND EVOLUTION},
  keywords     = {Ecology,Ecology,Evolution,Behavior and Systematics,Nature and Landscape Conservation,differential transcript profiles,Mediterranean conifer,pre-adapted genotypes,response strategies,water stress,PINE PINUS-PINASTER,MARITIME PINE,ABIOTIC-STRESS,GENE FAMILY,OSMOTIC ADJUSTMENT,USE EFFICIENCY,CLIMATE-CHANGE,GAS-EXCHANGE,AIT.,IDENTIFICATION},
  language     = {eng},
  number       = {18},
  pages        = {9788--9807},
  title        = {Molecular study of drought response in the Mediterranean conifer Pinus Pinaster Ait. : differential transcriptomic profiling reveals constitutive water deficit‐independent drought tolerance mechanisms},
  url          = {http://dx.doi.org/10.1002/ece3.6613},
  volume       = {10},
  year         = {2020},
}

Altmetric
View in Altmetric
Web of Science
Times cited: