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Structural evolution and tissue-specific expression of tetrapod-specific second isoform of secretory pathway Ca2+-ATPase

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Abstract
Secretory pathway Ca-ATPases are less characterized mammalian calcium pumps than plasma membrane Ca-ATPases and sarco-endoplasmic reticulum Ca-ATPases. Here we report analysis of molecular evolution, alternative splicing, tissue-specific expression and subcellular localization of the second isoform of the secretory pathway Ca-ATPase (SPCA2), the product of the ATP2C2 gene. The primary structure of SPCA2 from rat duodenum deduced from full-length transcript contains 944 amino acid residues, and exhibits 65% sequence identity with known SPCA1. The rat SPCA2 sequence is also highly homologous to putative human protein KIAA0703, however, the latter seems to have an aberrant N-terminus originating from intron 2. The tissue-specificity of SPCA2 expression is different from ubiquitous SPCA1. Rat SPCA2 transcripts were detected predominantly in gastrointestinal tract, lung, trachea, lactating mammary gland, skin and preputial gland. In the newborn pig, the expression profile is very similar with one remarkable exception: porcine bulbourethral gland gave the strongest signal. Upon overexpression in cultured cells, SPCA2 shows an intracellular distribution with remarkable enrichment in Golgi. However, in vivo SPCA2 may be localized in compartments that differ among various tissues: it is intracellular in epidermis, but enriched in plasma membranes of the intestinal epithelium. Analysis of SPCA2 sequences from various vertebrate species argue that ATP2C2 gene radiated from ATP2C1 (encoding SPCA1) during adaptation of tetrapod ancestors to terrestrial habitats. (C) 2012 Elsevier Inc. All rights reserved.
Keywords
PLASMA-MEMBRANE, CALCIUM PUMPS, ION-PUMP, YEAST, PMR1, CA2+, MUTATIONS, GOLGI, CA2+/MN2+-ATPASE, SELECTIVITY, Vertebrate evolution, Gene duplication, Enzyme isoforms, Golgi complex, Alternative splicing, CPCA2, Ca-ATPase

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MLA
Pestov, Nikolay B., et al. “Structural Evolution and Tissue-Specific Expression of Tetrapod-Specific Second Isoform of Secretory Pathway Ca2+-ATPase.” BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 417, no. 4, Academic Press Inc Elsevier Science, 2012, pp. 1298–303, doi:10.1016/j.bbrc.2011.12.135.
APA
Pestov, N. B., Dmitriev, R. I., Kostina, M. B., Korneenko, T. V., Shakhparonov, M. I., & Modyanov, N. N. (2012). Structural evolution and tissue-specific expression of tetrapod-specific second isoform of secretory pathway Ca2+-ATPase. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 417(4), 1298–1303. https://doi.org/10.1016/j.bbrc.2011.12.135
Chicago author-date
Pestov, Nikolay B., Ruslan I. Dmitriev, Maria B. Kostina, Tatyana V. Korneenko, Mikhail I. Shakhparonov, and Nikolai N. Modyanov. 2012. “Structural Evolution and Tissue-Specific Expression of Tetrapod-Specific Second Isoform of Secretory Pathway Ca2+-ATPase.” BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 417 (4): 1298–1303. https://doi.org/10.1016/j.bbrc.2011.12.135.
Chicago author-date (all authors)
Pestov, Nikolay B., Ruslan I. Dmitriev, Maria B. Kostina, Tatyana V. Korneenko, Mikhail I. Shakhparonov, and Nikolai N. Modyanov. 2012. “Structural Evolution and Tissue-Specific Expression of Tetrapod-Specific Second Isoform of Secretory Pathway Ca2+-ATPase.” BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 417 (4): 1298–1303. doi:10.1016/j.bbrc.2011.12.135.
Vancouver
1.
Pestov NB, Dmitriev RI, Kostina MB, Korneenko TV, Shakhparonov MI, Modyanov NN. Structural evolution and tissue-specific expression of tetrapod-specific second isoform of secretory pathway Ca2+-ATPase. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. 2012;417(4):1298–303.
IEEE
[1]
N. B. Pestov, R. I. Dmitriev, M. B. Kostina, T. V. Korneenko, M. I. Shakhparonov, and N. N. Modyanov, “Structural evolution and tissue-specific expression of tetrapod-specific second isoform of secretory pathway Ca2+-ATPase,” BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 417, no. 4, pp. 1298–1303, 2012.
@article{8687923,
  abstract     = {Secretory pathway Ca-ATPases are less characterized mammalian calcium pumps than plasma membrane Ca-ATPases and sarco-endoplasmic reticulum Ca-ATPases. Here we report analysis of molecular evolution, alternative splicing, tissue-specific expression and subcellular localization of the second isoform of the secretory pathway Ca-ATPase (SPCA2), the product of the ATP2C2 gene. The primary structure of SPCA2 from rat duodenum deduced from full-length transcript contains 944 amino acid residues, and exhibits 65% sequence identity with known SPCA1. The rat SPCA2 sequence is also highly homologous to putative human protein KIAA0703, however, the latter seems to have an aberrant N-terminus originating from intron 2. The tissue-specificity of SPCA2 expression is different from ubiquitous SPCA1. Rat SPCA2 transcripts were detected predominantly in gastrointestinal tract, lung, trachea, lactating mammary gland, skin and preputial gland. In the newborn pig, the expression profile is very similar with one remarkable exception: porcine bulbourethral gland gave the strongest signal. Upon overexpression in cultured cells, SPCA2 shows an intracellular distribution with remarkable enrichment in Golgi. However, in vivo SPCA2 may be localized in compartments that differ among various tissues: it is intracellular in epidermis, but enriched in plasma membranes of the intestinal epithelium. Analysis of SPCA2 sequences from various vertebrate species argue that ATP2C2 gene radiated from ATP2C1 (encoding SPCA1) during adaptation of tetrapod ancestors to terrestrial habitats. (C) 2012 Elsevier Inc. All rights reserved.},
  author       = {Pestov, Nikolay B. and Dmitriev, Ruslan I. and Kostina, Maria B. and Korneenko, Tatyana V. and Shakhparonov, Mikhail I. and Modyanov, Nikolai N.},
  issn         = {0006-291X},
  journal      = {BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS},
  keywords     = {PLASMA-MEMBRANE,CALCIUM PUMPS,ION-PUMP,YEAST,PMR1,CA2+,MUTATIONS,GOLGI,CA2+/MN2+-ATPASE,SELECTIVITY,Vertebrate evolution,Gene duplication,Enzyme isoforms,Golgi complex,Alternative splicing,CPCA2,Ca-ATPase},
  language     = {eng},
  number       = {4},
  pages        = {1298--1303},
  publisher    = {Academic Press Inc Elsevier Science},
  title        = {Structural evolution and tissue-specific expression of tetrapod-specific second isoform of secretory pathway Ca2+-ATPase},
  url          = {http://dx.doi.org/10.1016/j.bbrc.2011.12.135},
  volume       = {417},
  year         = {2012},
}

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