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Evolutionary diversification of the BetaM interactome acquired through co-option of the ATP1B4 gene in placental mammals

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Abstract
ATP1B4 genes represent a rare instance of orthologous vertebrate gene co-option that radically changed properties of the encoded BetaM proteins, which function as Na, K-ATPase subunits in lower vertebrates and birds. Eutherian BetaM has lost its ancestral function and became a muscle-specific resident of the inner nuclear membrane. Our earlier work implicated BetaM in regulation of gene expression through direct interaction with the transcriptional co-regulator SKIP. To gain insight into evolution of BetaM interactome we performed expanded screening of eutherian and avian cDNA libraries using yeast-two-hybrid and split-ubiquitin systems. The inventory of identified BetaM interactors includes lamina-associated protein LAP-1, myocyte nuclear envelope protein Syne1, BetaM itself, heme oxidases HMOX1 and HMOX2; transcription factor LZIP/CREB3, ERGIC3, PHF3, reticulocalbin-3, and beta-sarcoglycan. No new interactions were found for chicken BetaM and human Na, K-ATPase beta 1, beta 2 and beta 3 isoforms, indicating the uniqueness of eutherian BetaM interactome. Analysis of truncated forms of BetaM indicates that residues 72-98 adjacent to the membrane in nucleoplasmic domain are important for the interaction with SKIP. These findings demonstrate that evolutionary alterations in structural and functional properties of eutherian BetaM proteins are associated with the increase in its interactome complexity.
Keywords
INNER NUCLEAR-MEMBRANE, MUSCULAR-DYSTROPHY, SKELETAL-MUSCLE, SUBUNIT, FAMILY, STRUCTURAL MEMBER, M-PROTEIN, X, K-ATPASE, SARCOGLYCAN, LAMINA, MICE

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MLA
Korneenko, Tatyana V., et al. “Evolutionary Diversification of the BetaM Interactome Acquired through Co-Option of the ATP1B4 Gene in Placental Mammals.” SCIENTIFIC REPORTS, vol. 6, 2016, doi:10.1038/srep22395.
APA
Korneenko, T. V., Pestov, N. B., Ahmad, N., Okkelman, I. A., Dmitriev, R., Shakhparonov, M. I., & Modyanov, N. N. (2016). Evolutionary diversification of the BetaM interactome acquired through co-option of the ATP1B4 gene in placental mammals. SCIENTIFIC REPORTS, 6. https://doi.org/10.1038/srep22395
Chicago author-date
Korneenko, Tatyana V., Nikolay B. Pestov, Nisar Ahmad, Irina A. Okkelman, Ruslan Dmitriev, Mikhail I. Shakhparonov, and Nikolai N. Modyanov. 2016. “Evolutionary Diversification of the BetaM Interactome Acquired through Co-Option of the ATP1B4 Gene in Placental Mammals.” SCIENTIFIC REPORTS 6. https://doi.org/10.1038/srep22395.
Chicago author-date (all authors)
Korneenko, Tatyana V., Nikolay B. Pestov, Nisar Ahmad, Irina A. Okkelman, Ruslan Dmitriev, Mikhail I. Shakhparonov, and Nikolai N. Modyanov. 2016. “Evolutionary Diversification of the BetaM Interactome Acquired through Co-Option of the ATP1B4 Gene in Placental Mammals.” SCIENTIFIC REPORTS 6. doi:10.1038/srep22395.
Vancouver
1.
Korneenko TV, Pestov NB, Ahmad N, Okkelman IA, Dmitriev R, Shakhparonov MI, et al. Evolutionary diversification of the BetaM interactome acquired through co-option of the ATP1B4 gene in placental mammals. SCIENTIFIC REPORTS. 2016;6.
IEEE
[1]
T. V. Korneenko et al., “Evolutionary diversification of the BetaM interactome acquired through co-option of the ATP1B4 gene in placental mammals,” SCIENTIFIC REPORTS, vol. 6, 2016.
@article{8687882,
  abstract     = {ATP1B4 genes represent a rare instance of orthologous vertebrate gene co-option that radically changed properties of the encoded BetaM proteins, which function as Na, K-ATPase subunits in lower vertebrates and birds. Eutherian BetaM has lost its ancestral function and became a muscle-specific resident of the inner nuclear membrane. Our earlier work implicated BetaM in regulation of gene expression through direct interaction with the transcriptional co-regulator SKIP. To gain insight into evolution of BetaM interactome we performed expanded screening of eutherian and avian cDNA libraries using yeast-two-hybrid and split-ubiquitin systems. The inventory of identified BetaM interactors includes lamina-associated protein LAP-1, myocyte nuclear envelope protein Syne1, BetaM itself, heme oxidases HMOX1 and HMOX2; transcription factor LZIP/CREB3, ERGIC3, PHF3, reticulocalbin-3, and beta-sarcoglycan. No new interactions were found for chicken BetaM and human Na, K-ATPase beta 1, beta 2 and beta 3 isoforms, indicating the uniqueness of eutherian BetaM interactome. Analysis of truncated forms of BetaM indicates that residues 72-98 adjacent to the membrane in nucleoplasmic domain are important for the interaction with SKIP. These findings demonstrate that evolutionary alterations in structural and functional properties of eutherian BetaM proteins are associated with the increase in its interactome complexity.},
  articleno    = {22395},
  author       = {Korneenko, Tatyana V. and Pestov, Nikolay B. and Ahmad, Nisar and Okkelman, Irina A. and Dmitriev, Ruslan and Shakhparonov, Mikhail I. and Modyanov, Nikolai N.},
  issn         = {2045-2322},
  journal      = {SCIENTIFIC REPORTS},
  keywords     = {INNER NUCLEAR-MEMBRANE,MUSCULAR-DYSTROPHY,SKELETAL-MUSCLE,SUBUNIT,FAMILY,STRUCTURAL MEMBER,M-PROTEIN,X,K-ATPASE,SARCOGLYCAN,LAMINA,MICE},
  language     = {eng},
  pages        = {7},
  title        = {Evolutionary diversification of the BetaM interactome acquired through co-option of the ATP1B4 gene in placental mammals},
  url          = {http://dx.doi.org/10.1038/srep22395},
  volume       = {6},
  year         = {2016},
}

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