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Fabrication and handling of 3D scaffolds based on polymers and decellularized tissues

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Abstract
Polymeric, ceramic and hybrid material-based three-dimensional (3D) scaffold or matrix structures are important for successful tissue engineering. While the number of approaches utilizing the use of cell-based scaffold and matrix structures is constantly growing, it is essential to provide a framework of their typical preparation and evaluation for tissue engineering. This chapter describes the fabrication of 3D scaffolds using -two--photon polymerization, decellularization and cell encapsulation methods and easy-to-use protocols allowing assessing the cell morphology, cytotoxicity and viability in these scaffolds.
Keywords
ENGINEERING APPLICATIONS, 3-DIMENSIONAL SCAFFOLDS, CALCIUM-PHOSPHATE, BONE REGENERATION, HYDROGEL, CELLS, Decellularization, Fibrin gel, Microscopy, MTT-assay, Scaffolds, Two-photon polymerization

Citation

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MLA
Shpichka, Anastasia, et al. “Fabrication and Handling of 3D Scaffolds Based on Polymers and Decellularized Tissues.” Advances in Experimental Medicine and Biology, edited by Ruslan Dmitriev, vol. 1035, 2017, pp. 71–81, doi:10.1007/978-3-319-67358-5_5.
APA
Shpichka, A., Koroleva, A., Kuznetsova, D., Dmitriev, R., & Timashev, P. (2017). Fabrication and handling of 3D scaffolds based on polymers and decellularized tissues. Advances in Experimental Medicine and Biology, 1035, 71–81. https://doi.org/10.1007/978-3-319-67358-5_5
Chicago author-date
Shpichka, Anastasia, Anastasia Koroleva, Daria Kuznetsova, Ruslan Dmitriev, and Peter Timashev. 2017. “Fabrication and Handling of 3D Scaffolds Based on Polymers and Decellularized Tissues.” Edited by Ruslan Dmitriev. Advances in Experimental Medicine and Biology 1035: 71–81. https://doi.org/10.1007/978-3-319-67358-5_5.
Chicago author-date (all authors)
Shpichka, Anastasia, Anastasia Koroleva, Daria Kuznetsova, Ruslan Dmitriev, and Peter Timashev. 2017. “Fabrication and Handling of 3D Scaffolds Based on Polymers and Decellularized Tissues.” Ed by. Ruslan Dmitriev. Advances in Experimental Medicine and Biology 1035: 71–81. doi:10.1007/978-3-319-67358-5_5.
Vancouver
1.
Shpichka A, Koroleva A, Kuznetsova D, Dmitriev R, Timashev P. Fabrication and handling of 3D scaffolds based on polymers and decellularized tissues. Dmitriev R, editor. Advances in Experimental Medicine and Biology. 2017;1035:71–81.
IEEE
[1]
A. Shpichka, A. Koroleva, D. Kuznetsova, R. Dmitriev, and P. Timashev, “Fabrication and handling of 3D scaffolds based on polymers and decellularized tissues,” Advances in Experimental Medicine and Biology, vol. 1035, pp. 71–81, 2017.
@article{8687872,
  abstract     = {{Polymeric, ceramic and hybrid material-based three-dimensional (3D) scaffold or matrix structures are important for successful tissue engineering. While the number of approaches utilizing the use of cell-based scaffold and matrix structures is constantly growing, it is essential to provide a framework of their typical preparation and evaluation for tissue engineering. This chapter describes the fabrication of 3D scaffolds using -two--photon polymerization, decellularization and cell encapsulation methods and easy-to-use protocols allowing assessing the cell morphology, cytotoxicity and viability in these scaffolds.}},
  author       = {{Shpichka, Anastasia and Koroleva, Anastasia and Kuznetsova, Daria and Dmitriev, Ruslan and Timashev, Peter}},
  editor       = {{Dmitriev, Ruslan}},
  isbn         = {{9783319673578}},
  issn         = {{0065-2598}},
  journal      = {{Advances in Experimental Medicine and Biology}},
  keywords     = {{ENGINEERING APPLICATIONS,3-DIMENSIONAL SCAFFOLDS,CALCIUM-PHOSPHATE,BONE REGENERATION,HYDROGEL,CELLS,Decellularization,Fibrin gel,Microscopy,MTT-assay,Scaffolds,Two-photon polymerization}},
  language     = {{eng}},
  pages        = {{71--81}},
  title        = {{Fabrication and handling of 3D scaffolds based on polymers and decellularized tissues}},
  url          = {{http://dx.doi.org/10.1007/978-3-319-67358-5_5}},
  volume       = {{1035}},
  year         = {{2017}},
}

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