Fabrication and Handling of 3D Scaffolds Based on Polymers and Decellularized Tissues
- Author
- Anastasia Shpichka, Anastasia Koroleva, Daria Kuznetsova, Ruslan I. Dmitriev and Peter Timashev
- Organization
- Abstract
- Polymeric, ceramic and hybrid material-based three-dimensional (3D) scaffold or matrix structures are important for successful tissue engineering. While the number of approaches utilizing the use of cell-based scaffold and matrix structures is constantly growing, it is essential to provide a framework of their typical preparation and evaluation for tissue engineering. This chapter describes the fabrication of 3D scaffolds using -two--photon polymerization, decellularization and cell encapsulation methods and easy-to-use protocols allowing assessing the cell morphology, cytotoxicity and viability in these scaffolds.
- Keywords
- ENGINEERING APPLICATIONS, 3-DIMENSIONAL SCAFFOLDS, CALCIUM-PHOSPHATE, BONE REGENERATION, HYDROGEL, CELLS, Decellularization, Fibrin gel, Microscopy, MTT-assay, Scaffolds, Two-photon polymerization
Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-8687872
- MLA
- Shpichka, Anastasia, et al. “Fabrication and Handling of 3D Scaffolds Based on Polymers and Decellularized Tissues.” MULTI-PARAMETRIC LIVE CELL MICROSCOPY OF 3D TISSUE MODELS, vol. 1035, Springer International Publishing Ag, 2017, pp. 71–81, doi:10.1007/978-3-319-67358-5_5.
- APA
- Shpichka, A., Koroleva, A., Kuznetsova, D., Dmitriev, R. I., & Timashev, P. (2017). Fabrication and Handling of 3D Scaffolds Based on Polymers and Decellularized Tissues. MULTI-PARAMETRIC LIVE CELL MICROSCOPY OF 3D TISSUE MODELS, 1035, 71–81. https://doi.org/10.1007/978-3-319-67358-5_5
- Chicago author-date
- Shpichka, Anastasia, Anastasia Koroleva, Daria Kuznetsova, Ruslan I. Dmitriev, and Peter Timashev. 2017. “Fabrication and Handling of 3D Scaffolds Based on Polymers and Decellularized Tissues.” MULTI-PARAMETRIC LIVE CELL MICROSCOPY OF 3D TISSUE MODELS 1035: 71–81. https://doi.org/10.1007/978-3-319-67358-5_5.
- Chicago author-date (all authors)
- Shpichka, Anastasia, Anastasia Koroleva, Daria Kuznetsova, Ruslan I. Dmitriev, and Peter Timashev. 2017. “Fabrication and Handling of 3D Scaffolds Based on Polymers and Decellularized Tissues.” MULTI-PARAMETRIC LIVE CELL MICROSCOPY OF 3D TISSUE MODELS 1035: 71–81. doi:10.1007/978-3-319-67358-5_5.
- Vancouver
- 1.Shpichka A, Koroleva A, Kuznetsova D, Dmitriev RI, Timashev P. Fabrication and Handling of 3D Scaffolds Based on Polymers and Decellularized Tissues. MULTI-PARAMETRIC LIVE CELL MICROSCOPY OF 3D TISSUE MODELS. 2017;1035:71–81.
- IEEE
- [1]A. Shpichka, A. Koroleva, D. Kuznetsova, R. I. Dmitriev, and P. Timashev, “Fabrication and Handling of 3D Scaffolds Based on Polymers and Decellularized Tissues,” MULTI-PARAMETRIC LIVE CELL MICROSCOPY OF 3D TISSUE MODELS, vol. 1035, pp. 71–81, 2017.
@article{8687872,
abstract = {Polymeric, ceramic and hybrid material-based three-dimensional (3D) scaffold or matrix structures are important for successful tissue engineering. While the number of approaches utilizing the use of cell-based scaffold and matrix structures is constantly growing, it is essential to provide a framework of their typical preparation and evaluation for tissue engineering. This chapter describes the fabrication of 3D scaffolds using -two--photon polymerization, decellularization and cell encapsulation methods and easy-to-use protocols allowing assessing the cell morphology, cytotoxicity and viability in these scaffolds.},
author = {Shpichka, Anastasia and Koroleva, Anastasia and Kuznetsova, Daria and Dmitriev, Ruslan I. and Timashev, Peter},
isbn = {978-3-319-67358-5},
issn = {0065-2598},
journal = {MULTI-PARAMETRIC LIVE CELL MICROSCOPY OF 3D TISSUE MODELS},
keywords = {ENGINEERING APPLICATIONS,3-DIMENSIONAL SCAFFOLDS,CALCIUM-PHOSPHATE,BONE REGENERATION,HYDROGEL,CELLS,Decellularization,Fibrin gel,Microscopy,MTT-assay,Scaffolds,Two-photon polymerization},
language = {eng},
pages = {71--81},
publisher = {Springer International Publishing Ag},
title = {Fabrication and Handling of 3D Scaffolds Based on Polymers and Decellularized Tissues},
url = {http://dx.doi.org/10.1007/978-3-319-67358-5_5},
volume = {1035},
year = {2017},
}
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