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Multiparametric optical bioimaging reveals the fate of epoxy crosslinked biomeshes in the mouse subcutaneous implantation model

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Abstract
Biomeshes based on decellularized bovine pericardium (DBP) are widely used in reconstructive surgery due to their wide availability and the attractive biomechanical properties. However, their efficacy in clinical applications is often affected by the uncontrolled immunogenicity and proteolytic degradation. To address this issue, we present here in vivo multiparametric imaging analysis of epoxy crosslinked DBPs to reveal their fate after implantation. We first analyzed the structure of the crosslinked DBP using scanning electron microscopy and evaluated proteolytic stability and cytotoxicity. Next, using combination of fluorescence and hypoxia imaging, X-ray computed microtomography and histology techniques we studied the fate of DBPs after subcutaneous implantation in animals. Our approach revealed high resistance to biodegradation, gradual remodeling of a surrounding tissue forming the connective tissue capsule and calcification of crosslinked DBPs. These changes were concomitant to the development of hypoxia in the samples within 3 weeks after implantation and subsequent induction of angiogenesis and vascularization. Collectively, presented approach provides new insights on the transplantation of the epoxy crosslinked biomeshes, the risks associated with its applications in soft-tissue reconstruction and can be transferred to studies of other types of implants.
Keywords
BIOPROSTHETIC HEART-VALVE, BOVINE PERICARDIUM, BIOMECHANICAL, CHARACTERIZATION, LINKING CHARACTERISTICS, 3-DIMENSIONAL CELL, COLLAGEN, MATRIX, CALCIFICATION, SCAFFOLD, BIOCOMPATIBILITY, BIODEGRADATION, decellularized tissue, biomeshes, bovine pericardium, epoxy, crosslinking, hypoxia, in vivo biodegradation, optical bioimaging, PLIM

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MLA
Elagin, Vadim, et al. “Multiparametric Optical Bioimaging Reveals the Fate of Epoxy Crosslinked Biomeshes in the Mouse Subcutaneous Implantation Model.” FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY, vol. 8, Frontiers Media Sa, 2020, doi:10.3389/fbioe.2020.00107.
APA
Elagin, V., Kuznetsova, D., Grebenik, E., Zolotov, D. A., Istranov, L., Zharikova, T., … Timashev, P. (2020). Multiparametric optical bioimaging reveals the fate of epoxy crosslinked biomeshes in the mouse subcutaneous implantation model. FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY, 8. https://doi.org/10.3389/fbioe.2020.00107
Chicago author-date
Elagin, Vadim, Daria Kuznetsova, Ekaterina Grebenik, Denis A. Zolotov, Leonid Istranov, Tatiana Zharikova, Elena Istranova, et al. 2020. “Multiparametric Optical Bioimaging Reveals the Fate of Epoxy Crosslinked Biomeshes in the Mouse Subcutaneous Implantation Model.” FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY 8. https://doi.org/10.3389/fbioe.2020.00107.
Chicago author-date (all authors)
Elagin, Vadim, Daria Kuznetsova, Ekaterina Grebenik, Denis A. Zolotov, Leonid Istranov, Tatiana Zharikova, Elena Istranova, Anastasia Polozova, Dmitry Reunov, Alexandr Kurkov, Anatoly Shekhter, Elvira R. Gafarova, Victor Asadchikov, Sergey M. Borisov, Ruslan Dmitriev, Elena Zagaynova, and Peter Timashev. 2020. “Multiparametric Optical Bioimaging Reveals the Fate of Epoxy Crosslinked Biomeshes in the Mouse Subcutaneous Implantation Model.” FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY 8. doi:10.3389/fbioe.2020.00107.
Vancouver
1.
Elagin V, Kuznetsova D, Grebenik E, Zolotov DA, Istranov L, Zharikova T, et al. Multiparametric optical bioimaging reveals the fate of epoxy crosslinked biomeshes in the mouse subcutaneous implantation model. FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY. 2020;8.
IEEE
[1]
V. Elagin et al., “Multiparametric optical bioimaging reveals the fate of epoxy crosslinked biomeshes in the mouse subcutaneous implantation model,” FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY, vol. 8, 2020.
@article{8687851,
  abstract     = {Biomeshes based on decellularized bovine pericardium (DBP) are widely used in reconstructive surgery due to their wide availability and the attractive biomechanical properties. However, their efficacy in clinical applications is often affected by the uncontrolled immunogenicity and proteolytic degradation. To address this issue, we present here in vivo multiparametric imaging analysis of epoxy crosslinked DBPs to reveal their fate after implantation. We first analyzed the structure of the crosslinked DBP using scanning electron microscopy and evaluated proteolytic stability and cytotoxicity. Next, using combination of fluorescence and hypoxia imaging, X-ray computed microtomography and histology techniques we studied the fate of DBPs after subcutaneous implantation in animals. Our approach revealed high resistance to biodegradation, gradual remodeling of a surrounding tissue forming the connective tissue capsule and calcification of crosslinked DBPs. These changes were concomitant to the development of hypoxia in the samples within 3 weeks after implantation and subsequent induction of angiogenesis and vascularization. Collectively, presented approach provides new insights on the transplantation of the epoxy crosslinked biomeshes, the risks associated with its applications in soft-tissue reconstruction and can be transferred to studies of other types of implants.},
  articleno    = {107},
  author       = {Elagin, Vadim and Kuznetsova, Daria and Grebenik, Ekaterina and Zolotov, Denis A. and Istranov, Leonid and Zharikova, Tatiana and Istranova, Elena and Polozova, Anastasia and Reunov, Dmitry and Kurkov, Alexandr and Shekhter, Anatoly and Gafarova, Elvira R. and Asadchikov, Victor and Borisov, Sergey M. and Dmitriev, Ruslan and Zagaynova, Elena and Timashev, Peter},
  issn         = {2296-4185},
  journal      = {FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY},
  keywords     = {BIOPROSTHETIC HEART-VALVE,BOVINE PERICARDIUM,BIOMECHANICAL,CHARACTERIZATION,LINKING CHARACTERISTICS,3-DIMENSIONAL CELL,COLLAGEN,MATRIX,CALCIFICATION,SCAFFOLD,BIOCOMPATIBILITY,BIODEGRADATION,decellularized tissue,biomeshes,bovine pericardium,epoxy,crosslinking,hypoxia,in vivo biodegradation,optical bioimaging,PLIM},
  language     = {eng},
  pages        = {14},
  publisher    = {Frontiers Media Sa},
  title        = {Multiparametric optical bioimaging reveals the fate of epoxy crosslinked biomeshes in the mouse subcutaneous implantation model},
  url          = {http://dx.doi.org/10.3389/fbioe.2020.00107},
  volume       = {8},
  year         = {2020},
}

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