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The anti-microbial peptide (Lin-SB056-1)(2)-K reduces pro-inflammatory cytokine release through Interaction with Pseudomonas aeruginosa lipopolysaccharide

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Abstract
The ability of many anti-microbial peptides (AMPs) to modulate the host immune response has highlighted their possible therapeutic use to reduce uncontrolled inflammation during chronic infections. In the present study, we examined the anti-inflammatory potential of the semi-synthetic peptide lin-SB056-1 and its dendrimeric derivative (lin-SB056-1)(2)-K, which were previously found to have anti-microbial activity against Pseudomonas aeruginosa in in vivo-like models mimicking the challenging environment of chronically infected lungs (i.e., artificial sputum medium and 3-D lung mucosa model). The dendrimeric derivative exerted a stronger anti-inflammatory activity than its monomeric counterpart towards lung epithelial- and macrophage-cell lines stimulated with P. aeruginosa lipopolysaccharide (LPS), based on a marked decrease (up to 80%) in the LPS-induced production of different pro-inflammatory cytokines (i.e., IL-1 beta, IL-6 and IL-8). Accordingly, (lin-SB056-1)(2)-K exhibited a stronger LPS-binding affinity than its monomeric counterpart, thereby suggesting a role of peptide/LPS neutralizing interactions in the observed anti-inflammatory effect. Along with the anti-bacterial and anti-biofilm properties, the anti-inflammatory activity of (lin-SB056-1)(2)-K broadens its therapeutic potential in the context of chronic (biofilm-associated) infections.
Keywords
HOST-DEFENSE PEPTIDES, ANTIINFLAMMATORY ACTIVITIES, DENDRIMERIC PEPTIDE, BINDING, ANTIBACTERIAL, RESPONSES, anti-microbial peptide, dendrimeric peptide, Pseudomonas aeruginosa, LPS, anti-inflammatory activity

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MLA
Grassi, Lucia, et al. “The Anti-Microbial Peptide (Lin-SB056-1)(2)-K Reduces pro-Inflammatory Cytokine Release through Interaction with Pseudomonas Aeruginosa Lipopolysaccharide.” ANTIBIOTICS-BASEL, vol. 9, no. 9, 2020, doi:10.3390/antibiotics9090585.
APA
Grassi, L., Pompilio, A., Kaya, E., Rinaldi, A. C., Sanjust, E., Maisetta, G., … Esin, S. (2020). The anti-microbial peptide (Lin-SB056-1)(2)-K reduces pro-inflammatory cytokine release through Interaction with Pseudomonas aeruginosa lipopolysaccharide. ANTIBIOTICS-BASEL, 9(9). https://doi.org/10.3390/antibiotics9090585
Chicago author-date
Grassi, Lucia, Arianna Pompilio, Esingul Kaya, Andrea C. Rinaldi, Enrico Sanjust, Giuseppantonio Maisetta, Aurélie Crabbé, Giovanni Di Bonaventura, Giovanna Batoni, and Semih Esin. 2020. “The Anti-Microbial Peptide (Lin-SB056-1)(2)-K Reduces pro-Inflammatory Cytokine Release through Interaction with Pseudomonas Aeruginosa Lipopolysaccharide.” ANTIBIOTICS-BASEL 9 (9). https://doi.org/10.3390/antibiotics9090585.
Chicago author-date (all authors)
Grassi, Lucia, Arianna Pompilio, Esingul Kaya, Andrea C. Rinaldi, Enrico Sanjust, Giuseppantonio Maisetta, Aurélie Crabbé, Giovanni Di Bonaventura, Giovanna Batoni, and Semih Esin. 2020. “The Anti-Microbial Peptide (Lin-SB056-1)(2)-K Reduces pro-Inflammatory Cytokine Release through Interaction with Pseudomonas Aeruginosa Lipopolysaccharide.” ANTIBIOTICS-BASEL 9 (9). doi:10.3390/antibiotics9090585.
Vancouver
1.
Grassi L, Pompilio A, Kaya E, Rinaldi AC, Sanjust E, Maisetta G, et al. The anti-microbial peptide (Lin-SB056-1)(2)-K reduces pro-inflammatory cytokine release through Interaction with Pseudomonas aeruginosa lipopolysaccharide. ANTIBIOTICS-BASEL. 2020;9(9).
IEEE
[1]
L. Grassi et al., “The anti-microbial peptide (Lin-SB056-1)(2)-K reduces pro-inflammatory cytokine release through Interaction with Pseudomonas aeruginosa lipopolysaccharide,” ANTIBIOTICS-BASEL, vol. 9, no. 9, 2020.
@article{8687760,
  abstract     = {{The ability of many anti-microbial peptides (AMPs) to modulate the host immune response has highlighted their possible therapeutic use to reduce uncontrolled inflammation during chronic infections. In the present study, we examined the anti-inflammatory potential of the semi-synthetic peptide lin-SB056-1 and its dendrimeric derivative (lin-SB056-1)(2)-K, which were previously found to have anti-microbial activity against Pseudomonas aeruginosa in in vivo-like models mimicking the challenging environment of chronically infected lungs (i.e., artificial sputum medium and 3-D lung mucosa model). The dendrimeric derivative exerted a stronger anti-inflammatory activity than its monomeric counterpart towards lung epithelial- and macrophage-cell lines stimulated with P. aeruginosa lipopolysaccharide (LPS), based on a marked decrease (up to 80%) in the LPS-induced production of different pro-inflammatory cytokines (i.e., IL-1 beta, IL-6 and IL-8). Accordingly, (lin-SB056-1)(2)-K exhibited a stronger LPS-binding affinity than its monomeric counterpart, thereby suggesting a role of peptide/LPS neutralizing interactions in the observed anti-inflammatory effect. Along with the anti-bacterial and anti-biofilm properties, the anti-inflammatory activity of (lin-SB056-1)(2)-K broadens its therapeutic potential in the context of chronic (biofilm-associated) infections.}},
  articleno    = {{585}},
  author       = {{Grassi, Lucia and Pompilio, Arianna and Kaya, Esingul and Rinaldi, Andrea C. and Sanjust, Enrico and Maisetta, Giuseppantonio and Crabbé, Aurélie and Di Bonaventura, Giovanni and Batoni, Giovanna and Esin, Semih}},
  issn         = {{2079-6382}},
  journal      = {{ANTIBIOTICS-BASEL}},
  keywords     = {{HOST-DEFENSE PEPTIDES,ANTIINFLAMMATORY ACTIVITIES,DENDRIMERIC PEPTIDE,BINDING,ANTIBACTERIAL,RESPONSES,anti-microbial peptide,dendrimeric peptide,Pseudomonas aeruginosa,LPS,anti-inflammatory activity}},
  language     = {{eng}},
  number       = {{9}},
  pages        = {{14}},
  title        = {{The anti-microbial peptide (Lin-SB056-1)(2)-K reduces pro-inflammatory cytokine release through Interaction with Pseudomonas aeruginosa lipopolysaccharide}},
  url          = {{http://doi.org/10.3390/antibiotics9090585}},
  volume       = {{9}},
  year         = {{2020}},
}

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