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Charting extracellular transcriptomes in the human biofluid RNA atlas

Eva Hulstaert, Annelien Morlion (UGent) , Francisco Avila Cobos (UGent) , Kimberly Verniers (UGent) , Justine Nuytens (UGent) , Eveline Vanden Eynde (UGent) , Nurten Yigit (UGent) , Jasper Anckaert (UGent) , Anja Geerts (UGent) , Pieter Hindryckx (UGent) , et al.
(2020) CELL REPORTS. 33(13).
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Abstract
Extracellular RNAs present in biofluids have emerged as potential biomarkers for disease. Where most studies focus on blood-derived fluids, other biofluids may be more informative. We present an atlas of messenger, circular, and small RNA transcriptomes of a comprehensive collection of 20 human biofluids. By means of synthetic spike-in controls, we compare RNA content across biofluids, revealing a 10,000-fold difference in concentration. The circular RNA fraction is increased in most biofluids compared to tissues. Each biofluid transcriptome is enriched for RNA molecules derived from specific tissues and cell types. Our atlas enables an informed selection of the most relevant biofluid to monitor particular diseases. To verify the biomarker potential in these biofluids, four validation cohorts representing a broad spectrum of diseases were profiled, revealing numerous differential RNAs between case and control subjects. Spike-normalized data are publicly available in the R2 web portal for further exploration.
Keywords
MESSENGER-RNA, CIRCULAR RNA, EXPRESSION, LANDSCAPE, MICRORNA, BIOMARKERS

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MLA
Hulstaert, Eva, et al. “Charting Extracellular Transcriptomes in the Human Biofluid RNA Atlas.” CELL REPORTS, vol. 33, no. 13, 2020, doi:10.1016/j.celrep.2020.108552.
APA
Hulstaert, E., Morlion, A., Avila Cobos, F., Verniers, K., Nuytens, J., Vanden Eynde, E., … Mestdagh, P. (2020). Charting extracellular transcriptomes in the human biofluid RNA atlas. CELL REPORTS, 33(13). https://doi.org/10.1016/j.celrep.2020.108552
Chicago author-date
Hulstaert, Eva, Annelien Morlion, Francisco Avila Cobos, Kimberly Verniers, Justine Nuytens, Eveline Vanden Eynde, Nurten Yigit, et al. 2020. “Charting Extracellular Transcriptomes in the Human Biofluid RNA Atlas.” CELL REPORTS 33 (13). https://doi.org/10.1016/j.celrep.2020.108552.
Chicago author-date (all authors)
Hulstaert, Eva, Annelien Morlion, Francisco Avila Cobos, Kimberly Verniers, Justine Nuytens, Eveline Vanden Eynde, Nurten Yigit, Jasper Anckaert, Anja Geerts, Pieter Hindryckx, Peggy Jacques, Guy Brusselle, Ken Bracke, Tania Maes, Thomas Malfait, Thierry Derveaux, Virginie Ninclaus, Caroline Van Cauwenbergh, Kristien Roelens, Ellen Roets, Dimitri Hemelsoet, Kelly Tilleman, Lieve Brochez, S Kuersten, LM Simon, S Karg, A Kautzky-Willers, M Leutner, C Nöhammer, O Slaby, RW Prins, J Koster, Steve Lefever, GP Schroth, Jo Vandesompele, and Pieter Mestdagh. 2020. “Charting Extracellular Transcriptomes in the Human Biofluid RNA Atlas.” CELL REPORTS 33 (13). doi:10.1016/j.celrep.2020.108552.
Vancouver
1.
Hulstaert E, Morlion A, Avila Cobos F, Verniers K, Nuytens J, Vanden Eynde E, et al. Charting extracellular transcriptomes in the human biofluid RNA atlas. CELL REPORTS. 2020;33(13).
IEEE
[1]
E. Hulstaert et al., “Charting extracellular transcriptomes in the human biofluid RNA atlas,” CELL REPORTS, vol. 33, no. 13, 2020.
@article{8687628,
  abstract     = {{Extracellular RNAs present in biofluids have emerged as potential biomarkers for disease. Where most studies focus on blood-derived fluids, other biofluids may be more informative. We present an atlas of messenger, circular, and small RNA transcriptomes of a comprehensive collection of 20 human biofluids. By means of synthetic spike-in controls, we compare RNA content across biofluids, revealing a 10,000-fold difference in concentration. The circular RNA fraction is increased in most biofluids compared to tissues. Each biofluid transcriptome is enriched for RNA molecules derived from specific tissues and cell types. Our atlas enables an informed selection of the most relevant biofluid to monitor particular diseases. To verify the biomarker potential in these biofluids, four validation cohorts representing a broad spectrum of diseases were profiled, revealing numerous differential RNAs between case and control subjects. Spike-normalized data are publicly available in the R2 web portal for further exploration.}},
  articleno    = {{108552}},
  author       = {{Hulstaert, Eva and Morlion, Annelien and Avila Cobos, Francisco and Verniers, Kimberly and Nuytens, Justine and Vanden Eynde, Eveline and Yigit, Nurten and Anckaert, Jasper and Geerts, Anja and Hindryckx, Pieter and Jacques, Peggy and Brusselle, Guy and Bracke, Ken and Maes, Tania and Malfait, Thomas and Derveaux, Thierry and Ninclaus, Virginie and Van Cauwenbergh, Caroline and Roelens, Kristien and Roets, Ellen and Hemelsoet, Dimitri and Tilleman, Kelly and Brochez, Lieve and Kuersten, S and Simon, LM and Karg, S and Kautzky-Willers, A and Leutner, M and Nöhammer, C and Slaby, O and Prins, RW and Koster, J and Lefever, Steve and Schroth, GP and Vandesompele, Jo and Mestdagh, Pieter}},
  issn         = {{2211-1247}},
  journal      = {{CELL REPORTS}},
  keywords     = {{MESSENGER-RNA,CIRCULAR RNA,EXPRESSION,LANDSCAPE,MICRORNA,BIOMARKERS}},
  language     = {{eng}},
  number       = {{13}},
  pages        = {{19}},
  title        = {{Charting extracellular transcriptomes in the human biofluid RNA atlas}},
  url          = {{http://doi.org/10.1016/j.celrep.2020.108552}},
  volume       = {{33}},
  year         = {{2020}},
}
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