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Storage time and temperature affect the isolation rate of Mannheimia haemolytica and Pasteurella multocida from bovine bronchoalveolar lavage samples

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Abstract
Background A microbiological diagnosis is essential to better target antimicrobial treatment, control and prevention of respiratory tract infections in cattle. Under field conditions, non-endoscopic broncho-alveolar lavage (nBAL) samples are increasingly collected. To what extent the highly variable turnaround time and storage temperatures between sampling and cultivation affect the isolation rate of bacterial pathogens is unknown. Therefore, the objective of this experimental study was to determine the effect of different storage temperatures (0 degrees C, 8 degrees C, 23 degrees C and 36 degrees C) and times (0,2,4,6,8,24,48 h) on the isolation rate and concentration ofPasteurellaceaein nBAL samples from clinically affected animals. Results At a storage temperature temperature of 36 degrees C isolation rates ofMannheimia haemolyticaandPasteurella multocidawere significantly reduced 6 h and 48 h after sampling, respectively. At room temperature (23 degrees C), a decrease inM. haemolyticaandP. multocidaisolation rate was noticed, starting at 24 and 48 h after sampling, respectively, but only significant forP. multocidaat 48 h. The presence of microbial contamination negatively affected the isolation ofP. multocidain clinical nBAL samples, but not ofM. haemolytica. Conclusion OptimalM. haemolyticaandP. multocidaisolation rates from clinical nBAL samples are obtained after storage at 0 degrees C or 8 degrees C, provided that the sample is cultivated within 24 h after sampling. The maximum period a sample can be stored without an effect on theM. haemolyticaandP. multocidaisolation success varies and is dependent on the storage temperature and the degree of microbial contamination.
Keywords
broncho alveolar lavage Pasteurella diagnostics respiratory disease calves, Pasteurellaceae, Transport conditions, Bronchoalveolar lavage, Cattle, Bovine respiratory disease, MANNHEIMIA-HAEMOLYTICA, RESPIRATORY-DISEASE, CALVES, SWAB, PATHOGENS, PASTEURELLACEAE, SURVIVAL, GROWTH

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MLA
Van Driessche, Laura, et al. “Storage Time and Temperature Affect the Isolation Rate of Mannheimia Haemolytica and Pasteurella Multocida from Bovine Bronchoalveolar Lavage Samples.” BMC VETERINARY RESEARCH, vol. 16, no. 1, 2020, doi:10.1186/s12917-020-02456-7.
APA
Van Driessche, L., De Neve, C., Haesebrouck, F., van Leenen, K., Boyen, F., & Pardon, B. (2020). Storage time and temperature affect the isolation rate of Mannheimia haemolytica and Pasteurella multocida from bovine bronchoalveolar lavage samples. BMC VETERINARY RESEARCH, 16(1). https://doi.org/10.1186/s12917-020-02456-7
Chicago author-date
Van Driessche, Laura, Charlotte De Neve, Freddy Haesebrouck, Katharina van Leenen, Filip Boyen, and Bart Pardon. 2020. “Storage Time and Temperature Affect the Isolation Rate of Mannheimia Haemolytica and Pasteurella Multocida from Bovine Bronchoalveolar Lavage Samples.” BMC VETERINARY RESEARCH 16 (1). https://doi.org/10.1186/s12917-020-02456-7.
Chicago author-date (all authors)
Van Driessche, Laura, Charlotte De Neve, Freddy Haesebrouck, Katharina van Leenen, Filip Boyen, and Bart Pardon. 2020. “Storage Time and Temperature Affect the Isolation Rate of Mannheimia Haemolytica and Pasteurella Multocida from Bovine Bronchoalveolar Lavage Samples.” BMC VETERINARY RESEARCH 16 (1). doi:10.1186/s12917-020-02456-7.
Vancouver
1.
Van Driessche L, De Neve C, Haesebrouck F, van Leenen K, Boyen F, Pardon B. Storage time and temperature affect the isolation rate of Mannheimia haemolytica and Pasteurella multocida from bovine bronchoalveolar lavage samples. BMC VETERINARY RESEARCH. 2020;16(1).
IEEE
[1]
L. Van Driessche, C. De Neve, F. Haesebrouck, K. van Leenen, F. Boyen, and B. Pardon, “Storage time and temperature affect the isolation rate of Mannheimia haemolytica and Pasteurella multocida from bovine bronchoalveolar lavage samples,” BMC VETERINARY RESEARCH, vol. 16, no. 1, 2020.
@article{8686940,
  abstract     = {{Background A microbiological diagnosis is essential to better target antimicrobial treatment, control and prevention of respiratory tract infections in cattle. Under field conditions, non-endoscopic broncho-alveolar lavage (nBAL) samples are increasingly collected. To what extent the highly variable turnaround time and storage temperatures between sampling and cultivation affect the isolation rate of bacterial pathogens is unknown. Therefore, the objective of this experimental study was to determine the effect of different storage temperatures (0 degrees C, 8 degrees C, 23 degrees C and 36 degrees C) and times (0,2,4,6,8,24,48 h) on the isolation rate and concentration ofPasteurellaceaein nBAL samples from clinically affected animals. Results At a storage temperature temperature of 36 degrees C isolation rates ofMannheimia haemolyticaandPasteurella multocidawere significantly reduced 6 h and 48 h after sampling, respectively. At room temperature (23 degrees C), a decrease inM. haemolyticaandP. multocidaisolation rate was noticed, starting at 24 and 48 h after sampling, respectively, but only significant forP. multocidaat 48 h. The presence of microbial contamination negatively affected the isolation ofP. multocidain clinical nBAL samples, but not ofM. haemolytica. Conclusion OptimalM. haemolyticaandP. multocidaisolation rates from clinical nBAL samples are obtained after storage at 0 degrees C or 8 degrees C, provided that the sample is cultivated within 24 h after sampling. The maximum period a sample can be stored without an effect on theM. haemolyticaandP. multocidaisolation success varies and is dependent on the storage temperature and the degree of microbial contamination.}},
  articleno    = {{238}},
  author       = {{Van Driessche, Laura and De Neve, Charlotte and Haesebrouck, Freddy and van Leenen, Katharina and Boyen, Filip and Pardon, Bart}},
  issn         = {{1746-6148}},
  journal      = {{BMC VETERINARY RESEARCH}},
  keywords     = {{broncho alveolar lavage Pasteurella diagnostics respiratory disease calves,Pasteurellaceae,Transport conditions,Bronchoalveolar lavage,Cattle,Bovine respiratory disease,MANNHEIMIA-HAEMOLYTICA,RESPIRATORY-DISEASE,CALVES,SWAB,PATHOGENS,PASTEURELLACEAE,SURVIVAL,GROWTH}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{8}},
  title        = {{Storage time and temperature affect the isolation rate of Mannheimia haemolytica and Pasteurella multocida from bovine bronchoalveolar lavage samples}},
  url          = {{http://dx.doi.org/10.1186/s12917-020-02456-7}},
  volume       = {{16}},
  year         = {{2020}},
}

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