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Microbial and dietary factors associated with the Equol and 8-prenylnaringenin producer phenotypes

Selin Bolca UGent, Sam Possemiers UGent, Willy Verstraete UGent, Tom Van de Wiele UGent, Arne Heyerick UGent, Denis De Keukeleire UGent, Inge Huybrechts UGent, Stefaan De Henauw UGent, Herman Depypere UGent and Marc Bracke UGent (2009) Acta Horticulturae. 848. p.235-246
abstract
The importance of the gut microbiota in the phyto-oestrogen bioavailability and bioactivity has been convincingly demonstrated in vitro and in vivo. Equol, a microbial metabolite of the soy isoflavone daidzein, is more bioactive than its precursor, and has been hypothesized as a clue to the effectiveness of soy and its isoflavones. Analogously, the weakly oestrogenic hop prenylflavonoid isoxanthohumol can be demethylated to the very potent phyto-oestrogen 8-prenylnaringenin (8-PN). However, these bioactivations are characterised by large inter-individual variation. The question why these phyto-oestrogen-bioactivating microbial populations colonised only 30-50% of the population, remains unanswered. Therefore, microbial and dietary factors associated with the ability to produce equol or 8-PN were studied in a dietary intervention trial with 150 healthy menopausal women. After a 4-d washout, subjects delivered urine and faecal samples. Next, they started a 5-d treatment with either soymilk, soy germ or hop extract and collected on the last day 24-h urine samples. A validated food-frequency questionnaire was used to estimate the usual fat (total fat, saturated, mono and polyunsaturated fatty acid), fibre, alcohol, caffeine, and theobromine intakes. Based on the urinary excretion profiles subjects were phenotyped as poor, moderate and strong equol or 8-PN producers. The equol production correlated negatively with Clostridium coccoides - Eubacterium rectale counts and positively with the abundance of sulphate-reducing bacteria. Recent antibiotic therapy seemed to affect the 8-PN production negatively. Furthermore, strong equol producers reported higher poly-unsaturated fatty acid and alcohol intakes, whereas strong 8-PN producers consumed less alcohol and more theobromine.
Please use this url to cite or link to this publication:
author
organization
year
type
conference
publication status
published
subject
keyword
soy, LIQUID-CHROMATOGRAPHY, hops, phyto-oestrogens, gut flora, microbial bio-activation, dietary intervention, HUMULUS-LUPULUS L., FOOD-FREQUENCY QUESTIONNAIRE, POSTMENOPAUSAL WOMEN, PHYTO-ESTROGENS, METABOLIZING PHENOTYPES, PREMENOPAUSAL WOMEN, URINARY-EXCRETION, LIVER-MICROSOMES, POTENT PHYTOESTROGEN
in
Acta Horticulturae
Acta Hortic.
editor
Denis De Keukeleire UGent and KE Hummer
volume
848
issue title
Proceedings of the second international humulus symposium
pages
235 - 246
publisher
International Society for Horticultural Science (ISHS)
place of publication
Leuven, Belgium
conference name
2nd International Humulus Symposium
conference location
Ghent, Belgium
conference start
2008-09-01
conference end
2008-09-05
Web of Science type
Proceedings Paper
Web of Science id
000291668300025
ISSN
0567-7572
ISBN
9789066057227
language
English
UGent publication?
yes
classification
P1
copyright statement
I have transferred the copyright for this publication to the publisher
VABB id
c:vabb:299136
VABB type
VABB-5
id
868531
handle
http://hdl.handle.net/1854/LU-868531
alternative location
http://www.actahort.org/books/848/848_25.htm
date created
2010-02-19 08:38:49
date last changed
2011-07-14 09:03:06
@inproceedings{868531,
  abstract     = {The importance of the gut microbiota in the phyto-oestrogen bioavailability and bioactivity has been convincingly demonstrated in vitro and in vivo. Equol, a microbial metabolite of the soy isoflavone daidzein, is more bioactive than its precursor, and has been hypothesized as a clue to the effectiveness of soy and its isoflavones. Analogously, the weakly oestrogenic hop prenylflavonoid isoxanthohumol can be demethylated to the very potent phyto-oestrogen 8-prenylnaringenin (8-PN). However, these bioactivations are characterised by large inter-individual variation. The question why these phyto-oestrogen-bioactivating microbial populations colonised only 30-50\% of the population, remains unanswered. Therefore, microbial and dietary factors associated with the ability to produce equol or 8-PN were studied in a dietary intervention trial with 150 healthy menopausal women. After a 4-d washout, subjects delivered urine and faecal samples. Next, they started a 5-d treatment with either soymilk, soy germ or hop extract and collected on the last day 24-h urine samples. A validated food-frequency questionnaire was used to estimate the usual fat (total fat, saturated, mono and polyunsaturated fatty acid), fibre, alcohol, caffeine, and theobromine intakes. Based on the urinary excretion profiles subjects were phenotyped as poor, moderate and strong equol or 8-PN producers. The equol production correlated negatively with Clostridium coccoides - Eubacterium rectale counts and positively with the abundance of sulphate-reducing bacteria. Recent antibiotic therapy seemed to affect the 8-PN production negatively. Furthermore, strong equol producers reported higher poly-unsaturated fatty acid and alcohol intakes, whereas strong 8-PN producers consumed less alcohol and more theobromine.},
  author       = {Bolca, Selin and Possemiers, Sam and Verstraete, Willy and Van de Wiele, Tom and Heyerick, Arne and De Keukeleire, Denis and Huybrechts, Inge and De Henauw, Stefaan and Depypere, Herman and Bracke, Marc},
  booktitle    = {Acta Horticulturae},
  editor       = {De Keukeleire, Denis and Hummer, KE},
  isbn         = {9789066057227},
  issn         = {0567-7572},
  keyword      = {soy,LIQUID-CHROMATOGRAPHY,hops,phyto-oestrogens,gut flora,microbial bio-activation,dietary intervention,HUMULUS-LUPULUS L.,FOOD-FREQUENCY QUESTIONNAIRE,POSTMENOPAUSAL WOMEN,PHYTO-ESTROGENS,METABOLIZING PHENOTYPES,PREMENOPAUSAL WOMEN,URINARY-EXCRETION,LIVER-MICROSOMES,POTENT PHYTOESTROGEN},
  language     = {eng},
  location     = {Ghent, Belgium},
  pages        = {235--246},
  publisher    = {International Society for Horticultural Science (ISHS)},
  title        = {Microbial and dietary factors associated with the Equol and 8-prenylnaringenin producer phenotypes},
  url          = {http://www.actahort.org/books/848/848\_25.htm},
  volume       = {848},
  year         = {2009},
}

Chicago
Bolca, Selin, Sam Possemiers, Willy Verstraete, Tom Van de Wiele, Arne Heyerick, Denis De Keukeleire, Inge Huybrechts, Stefaan De Henauw, Herman Depypere, and Marc Bracke. 2009. “Microbial and Dietary Factors Associated with the Equol and 8-prenylnaringenin Producer Phenotypes.” In Acta Horticulturae, ed. Denis De Keukeleire and KE Hummer, 848:235–246. Leuven, Belgium: International Society for Horticultural Science (ISHS).
APA
Bolca, S., Possemiers, S., Verstraete, W., Van de Wiele, T., Heyerick, A., De Keukeleire, D., Huybrechts, I., et al. (2009). Microbial and dietary factors associated with the Equol and 8-prenylnaringenin producer phenotypes. In D. De Keukeleire & K. Hummer (Eds.), Acta Horticulturae (Vol. 848, pp. 235–246). Presented at the 2nd International Humulus Symposium, Leuven, Belgium: International Society for Horticultural Science (ISHS).
Vancouver
1.
Bolca S, Possemiers S, Verstraete W, Van de Wiele T, Heyerick A, De Keukeleire D, et al. Microbial and dietary factors associated with the Equol and 8-prenylnaringenin producer phenotypes. In: De Keukeleire D, Hummer K, editors. Acta Horticulturae. Leuven, Belgium: International Society for Horticultural Science (ISHS); 2009. p. 235–46.
MLA
Bolca, Selin, Sam Possemiers, Willy Verstraete, et al. “Microbial and Dietary Factors Associated with the Equol and 8-prenylnaringenin Producer Phenotypes.” Acta Horticulturae. Ed. Denis De Keukeleire & KE Hummer. Vol. 848. Leuven, Belgium: International Society for Horticultural Science (ISHS), 2009. 235–246. Print.