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During a bioprospection of bacteria with antimicrobial activity, the actinomycete strain A38(T) was isolated from a sediment sample of the Carpintero river located in the Gran Piedra Mountains, Santiago de Cuba province (Cuba). This strain was identified as a member of the genus Micromonospora by means of a polyphasic taxonomy study. Strain A38(T) was an aerobic Gram-positive filamentous bacterium that produced single spores in a well-developed vegetative mycelium. An aerial mycelium was absent. The cell wall contained meso-diaminopimelic acid and the whole-cell sugars were glucose, mannose, ribose and xylose. The major cellular fatty acids were isoC(15:0), 10 methyl C-17:0, anteiso-C(17:)0 and iso-C-17:0. The predominant menaquinones were MK-10(H-4) and MK-10(H6). Phylogenetic analysis of 16S rRNA gene sequences revealed that this strain was closely related to Micromonospora tulbaghiae DSM 45142(T) (99.5%), Micromonospora citrea DSM 43903(T) (99.4 %), Micromonospora marina DSM 45555(T) (99.4 %), Micromonospora maritima DSM 45782(T) (99.3 %), Micromonospora sediminicola DSM 45794(T) (99.3%), Micromonospora aurantiaca DSM 43813(T) (99.2 %) and Micromonospora chaiyaphumensis DSM 45246(T) (99.2%). The results of OrthoANIu analysis showed the highest similarity to Micromonospora chalcea DSM 43026(T) (96.4%). However, the 16S rRNA and gyrB gene sequence-based phylogeny and phenotypic characteristics provided support to distinguish strain A38T as a novel species. On the basis of the results presented here, we propose to classify strain A38(T) (=LMG 30467(T)=CECT 30034(T)) as the type strain of the novel species Micromonospora fluminis sp. nov.
Keywords
Ecology, Evolution, Behavior and Systematics, Microbiology, General Medicine, Micromonospora, freshwater environment, polyphasic taxonomy, WGS, OrthoANIu, dDDH, GENUS, PHYLOGENIES, ALGORITHM, SEQUENCES, DATABASE, SOIL

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MLA
Camacho Pozo, Miladis Isabel, et al. “Micromonospora Fluminis Sp. Nov., Isolated from Mountain River Sediment.” INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, vol. 70, no. 12, 2020, pp. 6428–36, doi:10.1099/ijsem.0.004554.
APA
Camacho Pozo, M. I., Wieme, A., Rodríguez Pérez, S., Llauradó Maury, G., Peeters, C., Snauwaert, C., … Vandamme, P. (2020). Micromonospora fluminis sp. nov., isolated from mountain river sediment. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 70(12), 6428–6436. https://doi.org/10.1099/ijsem.0.004554
Chicago author-date
Camacho Pozo, Miladis Isabel, Anneleen Wieme, Suyén Rodríguez Pérez, Gabriel Llauradó Maury, Charlotte Peeters, Cindy Snauwaert, Yaneisy Lescaylle Veranes, Leonor Peña Zamora, Peter Schumann, and Peter Vandamme. 2020. “Micromonospora Fluminis Sp. Nov., Isolated from Mountain River Sediment.” INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 70 (12): 6428–36. https://doi.org/10.1099/ijsem.0.004554.
Chicago author-date (all authors)
Camacho Pozo, Miladis Isabel, Anneleen Wieme, Suyén Rodríguez Pérez, Gabriel Llauradó Maury, Charlotte Peeters, Cindy Snauwaert, Yaneisy Lescaylle Veranes, Leonor Peña Zamora, Peter Schumann, and Peter Vandamme. 2020. “Micromonospora Fluminis Sp. Nov., Isolated from Mountain River Sediment.” INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 70 (12): 6428–6436. doi:10.1099/ijsem.0.004554.
Vancouver
1.
Camacho Pozo MI, Wieme A, Rodríguez Pérez S, Llauradó Maury G, Peeters C, Snauwaert C, et al. Micromonospora fluminis sp. nov., isolated from mountain river sediment. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY. 2020;70(12):6428–36.
IEEE
[1]
M. I. Camacho Pozo et al., “Micromonospora fluminis sp. nov., isolated from mountain river sediment,” INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, vol. 70, no. 12, pp. 6428–6436, 2020.
@article{8685296,
  abstract     = {During a bioprospection of bacteria with antimicrobial activity, the actinomycete strain A38(T) was isolated from a sediment sample of the Carpintero river located in the Gran Piedra Mountains, Santiago de Cuba province (Cuba). This strain was identified as a member of the genus Micromonospora by means of a polyphasic taxonomy study. Strain A38(T) was an aerobic Gram-positive filamentous bacterium that produced single spores in a well-developed vegetative mycelium. An aerial mycelium was absent. The cell wall contained meso-diaminopimelic acid and the whole-cell sugars were glucose, mannose, ribose and xylose. The major cellular fatty acids were isoC(15:0), 10 methyl C-17:0, anteiso-C(17:)0 and iso-C-17:0. The predominant menaquinones were MK-10(H-4) and MK-10(H6). Phylogenetic analysis of 16S rRNA gene sequences revealed that this strain was closely related to Micromonospora tulbaghiae DSM 45142(T) (99.5%), Micromonospora citrea DSM 43903(T) (99.4 %), Micromonospora marina DSM 45555(T) (99.4 %), Micromonospora maritima DSM 45782(T) (99.3 %), Micromonospora sediminicola DSM 45794(T) (99.3%), Micromonospora aurantiaca DSM 43813(T) (99.2 %) and Micromonospora chaiyaphumensis DSM 45246(T) (99.2%). The results of OrthoANIu analysis showed the highest similarity to Micromonospora chalcea DSM 43026(T) (96.4%). However, the 16S rRNA and gyrB gene sequence-based phylogeny and phenotypic characteristics provided support to distinguish strain A38T as a novel species. On the basis of the results presented here, we propose to classify strain A38(T) (=LMG 30467(T)=CECT 30034(T)) as the type strain of the novel species Micromonospora fluminis sp. nov.},
  author       = {Camacho Pozo, Miladis Isabel and Wieme, Anneleen and Rodríguez Pérez, Suyén and Llauradó Maury, Gabriel and Peeters, Charlotte and Snauwaert, Cindy and Lescaylle Veranes, Yaneisy and Peña Zamora, Leonor and Schumann, Peter and Vandamme, Peter},
  issn         = {1466-5026},
  journal      = {INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY},
  keywords     = {Ecology,Evolution,Behavior and Systematics,Microbiology,General Medicine,Micromonospora,freshwater environment,polyphasic taxonomy,WGS,OrthoANIu,dDDH,GENUS,PHYLOGENIES,ALGORITHM,SEQUENCES,DATABASE,SOIL},
  language     = {eng},
  number       = {12},
  pages        = {6428--6436},
  title        = {Micromonospora fluminis sp. nov., isolated from mountain river sediment},
  url          = {http://dx.doi.org/10.1099/ijsem.0.004554},
  volume       = {70},
  year         = {2020},
}

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