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Occurrence, molecular characteristics, and antimicrobial resistance of escherichia Coli O157 in cattle, beef, and humans in Bishoftu Town, Central Ethiopia

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Abstract
Escherichia coliO157 is a Shiga toxin-producingE. colicausing disease in humans. Cattle are the primary reservoir of the pathogen. Information regarding the contribution of cattle to diarrheal illnesses in humans through consumption of contaminated beef is scarce in Ethiopia. We collected samples from 240 cattle, 127 beef, and 216 diarrheic patients in Bishoftu town in Ethiopia to assess the occurrence and determine the virulence genes, genetic relatedness, and antimicrobial resistance ofE. coliO157.E. coliO157 was detected in 7.1% of the rectal content samples from cattle in slaughterhouses, in 6.3% (n = 127) of the beef samples, and in 2.8% of the diarrheic patients' stool samples. All isolates were positive foreaegene, 24 (77%) of them were positive forstx2 gene (21stx2c and 3stx2a), whereasstx1 gene was not detected. Molecular typing grouped the isolates into eight pulsed-field gel electrophoresis pulsotypes with three pulsotypes containing isolates from all three sources, one pulsotype containing one isolate from human origin and one isolate from beef. The remaining four pulsotypes contained isolates unique either to beef or to humans. With the exception of 1 multidrug-resistant isolate from beef, which was resistant to 8 antimicrobial drugs, the remaining 30 isolates were susceptible to the 14 antimicrobials tested. In conclusion, the finding of genetically similar isolates in cattle, beef, and humans may indicate a potential transmission ofE. coliO157 from cattle to humans through beef. However, more robust studies are required to confirm this epidemiological link.
Keywords
Food Science, Animal Science and Zoology, Applied Microbiology and Biotechnology, Microbiology, E, coliO157, molecular characteristics, antimicrobial resistance, cattle, beef, humans, ENTEROHEMORRHAGIC ESCHERICHIA-COLI, VIRULENCE FACTORS, O157 PREVALENCE, GENES, CONTAMINATION, EPIDEMIOLOGY, INFECTIONS, SEROTYPES, CARCASSES, PRODUCTS

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MLA
Desissa Gutema, Fanta, et al. “Occurrence, Molecular Characteristics, and Antimicrobial Resistance of Escherichia Coli O157 in Cattle, Beef, and Humans in Bishoftu Town, Central Ethiopia.” FOODBORNE PATHOGENS AND DISEASE, vol. 18, no. 1, 2021, doi:10.1089/fpd.2020.2830.
APA
Desissa Gutema, F., Rasschaert, G., Agga, G. E., Jufare, A., Duguma, A. B., Abdi, R. D., … De Zutter, L. (2021). Occurrence, molecular characteristics, and antimicrobial resistance of escherichia Coli O157 in cattle, beef, and humans in Bishoftu Town, Central Ethiopia. FOODBORNE PATHOGENS AND DISEASE, 18(1). https://doi.org/10.1089/fpd.2020.2830
Chicago author-date
Desissa Gutema, Fanta, Geertrui Rasschaert, Getahun E. Agga, Alemnesh Jufare, Addisu B. Duguma, Reta D. Abdi, Luc Duchateau, Florence Crombe, Sarah Gabriël, and Lieven De Zutter. 2021. “Occurrence, Molecular Characteristics, and Antimicrobial Resistance of Escherichia Coli O157 in Cattle, Beef, and Humans in Bishoftu Town, Central Ethiopia.” FOODBORNE PATHOGENS AND DISEASE 18 (1). https://doi.org/10.1089/fpd.2020.2830.
Chicago author-date (all authors)
Desissa Gutema, Fanta, Geertrui Rasschaert, Getahun E. Agga, Alemnesh Jufare, Addisu B. Duguma, Reta D. Abdi, Luc Duchateau, Florence Crombe, Sarah Gabriël, and Lieven De Zutter. 2021. “Occurrence, Molecular Characteristics, and Antimicrobial Resistance of Escherichia Coli O157 in Cattle, Beef, and Humans in Bishoftu Town, Central Ethiopia.” FOODBORNE PATHOGENS AND DISEASE 18 (1). doi:10.1089/fpd.2020.2830.
Vancouver
1.
Desissa Gutema F, Rasschaert G, Agga GE, Jufare A, Duguma AB, Abdi RD, et al. Occurrence, molecular characteristics, and antimicrobial resistance of escherichia Coli O157 in cattle, beef, and humans in Bishoftu Town, Central Ethiopia. FOODBORNE PATHOGENS AND DISEASE. 2021;18(1).
IEEE
[1]
F. Desissa Gutema et al., “Occurrence, molecular characteristics, and antimicrobial resistance of escherichia Coli O157 in cattle, beef, and humans in Bishoftu Town, Central Ethiopia,” FOODBORNE PATHOGENS AND DISEASE, vol. 18, no. 1, 2021.
@article{8684327,
  abstract     = {{Escherichia coliO157 is a Shiga toxin-producingE. colicausing disease in humans. Cattle are the primary reservoir of the pathogen. Information regarding the contribution of cattle to diarrheal illnesses in humans through consumption of contaminated beef is scarce in Ethiopia. We collected samples from 240 cattle, 127 beef, and 216 diarrheic patients in Bishoftu town in Ethiopia to assess the occurrence and determine the virulence genes, genetic relatedness, and antimicrobial resistance ofE. coliO157.E. coliO157 was detected in 7.1% of the rectal content samples from cattle in slaughterhouses, in 6.3% (n = 127) of the beef samples, and in 2.8% of the diarrheic patients' stool samples. All isolates were positive foreaegene, 24 (77%) of them were positive forstx2 gene (21stx2c and 3stx2a), whereasstx1 gene was not detected. Molecular typing grouped the isolates into eight pulsed-field gel electrophoresis pulsotypes with three pulsotypes containing isolates from all three sources, one pulsotype containing one isolate from human origin and one isolate from beef. The remaining four pulsotypes contained isolates unique either to beef or to humans. With the exception of 1 multidrug-resistant isolate from beef, which was resistant to 8 antimicrobial drugs, the remaining 30 isolates were susceptible to the 14 antimicrobials tested. In conclusion, the finding of genetically similar isolates in cattle, beef, and humans may indicate a potential transmission ofE. coliO157 from cattle to humans through beef. However, more robust studies are required to confirm this epidemiological link.}},
  author       = {{Desissa Gutema, Fanta and Rasschaert, Geertrui and Agga, Getahun E. and Jufare, Alemnesh and Duguma, Addisu B. and Abdi, Reta D. and Duchateau, Luc and Crombe, Florence and Gabriël, Sarah and De Zutter, Lieven}},
  issn         = {{1535-3141}},
  journal      = {{FOODBORNE PATHOGENS AND DISEASE}},
  keywords     = {{Food Science,Animal Science and Zoology,Applied Microbiology and Biotechnology,Microbiology,E,coliO157,molecular characteristics,antimicrobial resistance,cattle,beef,humans,ENTEROHEMORRHAGIC ESCHERICHIA-COLI,VIRULENCE FACTORS,O157 PREVALENCE,GENES,CONTAMINATION,EPIDEMIOLOGY,INFECTIONS,SEROTYPES,CARCASSES,PRODUCTS}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{7}},
  title        = {{Occurrence, molecular characteristics, and antimicrobial resistance of escherichia Coli O157 in cattle, beef, and humans in Bishoftu Town, Central Ethiopia}},
  url          = {{http://dx.doi.org/10.1089/fpd.2020.2830}},
  volume       = {{18}},
  year         = {{2021}},
}

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