
The screening for novel inhibitors of auxin-induced Ca2+ signaling
(2021)
Plant chemical genomics : methods and protocols.
In Methods in Molecular Biology
2213.
p.89-98
- Author
- Kjell De Vriese, Long Nguyen (UGent) , Simon Stael (UGent) , Dominique Audenaert (UGent) , Tom Beeckman (UGent) and Steffen Vanneste (UGent)
- Organization
- Abstract
- Ca2+-based second messenger signaling is used by many signal perception mechanisms to modulate specific cellular responses. The well-characterized phytohormone auxin elicits a very rapid Ca2+ signal, but the molecular players involved in auxin-induced Ca2+ signaling are still largely unknown. The complicated and often redundant nature of the plant Ca2+ signaling machinery makes the use of mutants and transgenic lines a painstaking process, which makes a pharmacological approach an attractive alternative to study these processes. Here, we describe the development and utilization of a screening assay that can be used to probe a compound library for inhibitors of auxin-induced Ca2+ entry in plant cell suspensions.
- Keywords
- Auxin signaling, Auxin-induced calcium signaling, Calcium signaling, Inhibitors
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-8683262
- MLA
- De Vriese, Kjell, et al. “The Screening for Novel Inhibitors of Auxin-Induced Ca2+ Signaling.” Plant Chemical Genomics : Methods and Protocols, edited by Glenn R. Hicks and Chunhua Zhang, vol. 2213, Springer, 2021, pp. 89–98, doi:10.1007/978-1-0716-0954-5_8.
- APA
- De Vriese, K., Nguyen, L., Stael, S., Audenaert, D., Beeckman, T., & Vanneste, S. (2021). The screening for novel inhibitors of auxin-induced Ca2+ signaling. In G. R. Hicks & C. Zhang (Eds.), Plant chemical genomics : methods and protocols (Vol. 2213, pp. 89–98). https://doi.org/10.1007/978-1-0716-0954-5_8
- Chicago author-date
- De Vriese, Kjell, Long Nguyen, Simon Stael, Dominique Audenaert, Tom Beeckman, and Steffen Vanneste. 2021. “The Screening for Novel Inhibitors of Auxin-Induced Ca2+ Signaling.” In Plant Chemical Genomics : Methods and Protocols, edited by Glenn R. Hicks and Chunhua Zhang, 2213:89–98. Springer. https://doi.org/10.1007/978-1-0716-0954-5_8.
- Chicago author-date (all authors)
- De Vriese, Kjell, Long Nguyen, Simon Stael, Dominique Audenaert, Tom Beeckman, and Steffen Vanneste. 2021. “The Screening for Novel Inhibitors of Auxin-Induced Ca2+ Signaling.” In Plant Chemical Genomics : Methods and Protocols, ed by. Glenn R. Hicks and Chunhua Zhang, 2213:89–98. Springer. doi:10.1007/978-1-0716-0954-5_8.
- Vancouver
- 1.De Vriese K, Nguyen L, Stael S, Audenaert D, Beeckman T, Vanneste S. The screening for novel inhibitors of auxin-induced Ca2+ signaling. In: Hicks GR, Zhang C, editors. Plant chemical genomics : methods and protocols. Springer; 2021. p. 89–98.
- IEEE
- [1]K. De Vriese, L. Nguyen, S. Stael, D. Audenaert, T. Beeckman, and S. Vanneste, “The screening for novel inhibitors of auxin-induced Ca2+ signaling,” in Plant chemical genomics : methods and protocols, vol. 2213, G. R. Hicks and C. Zhang, Eds. Springer, 2021, pp. 89–98.
@incollection{8683262, abstract = {{Ca2+-based second messenger signaling is used by many signal perception mechanisms to modulate specific cellular responses. The well-characterized phytohormone auxin elicits a very rapid Ca2+ signal, but the molecular players involved in auxin-induced Ca2+ signaling are still largely unknown. The complicated and often redundant nature of the plant Ca2+ signaling machinery makes the use of mutants and transgenic lines a painstaking process, which makes a pharmacological approach an attractive alternative to study these processes. Here, we describe the development and utilization of a screening assay that can be used to probe a compound library for inhibitors of auxin-induced Ca2+ entry in plant cell suspensions.}}, author = {{De Vriese, Kjell and Nguyen, Long and Stael, Simon and Audenaert, Dominique and Beeckman, Tom and Vanneste, Steffen}}, booktitle = {{Plant chemical genomics : methods and protocols}}, editor = {{Hicks, Glenn R. and Zhang, Chunhua}}, isbn = {{9781071609538}}, issn = {{1064-3745}}, keywords = {{Auxin signaling,Auxin-induced calcium signaling,Calcium signaling,Inhibitors}}, language = {{eng}}, pages = {{89--98}}, publisher = {{Springer}}, series = {{Methods in Molecular Biology}}, title = {{The screening for novel inhibitors of auxin-induced Ca2+ signaling}}, url = {{http://doi.org/10.1007/978-1-0716-0954-5_8}}, volume = {{2213}}, year = {{2021}}, }
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