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The screening for novel inhibitors of auxin-induced Ca2+ signaling

Kjell De Vriese, Long Nguyen (UGent) , Simon Stael (UGent) , Dominique Audenaert (UGent) , Tom Beeckman (UGent) and Steffen Vanneste (UGent)
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Abstract
Ca2+-based second messenger signaling is used by many signal perception mechanisms to modulate specific cellular responses. The well-characterized phytohormone auxin elicits a very rapid Ca2+ signal, but the molecular players involved in auxin-induced Ca2+ signaling are still largely unknown. The complicated and often redundant nature of the plant Ca2+ signaling machinery makes the use of mutants and transgenic lines a painstaking process, which makes a pharmacological approach an attractive alternative to study these processes. Here, we describe the development and utilization of a screening assay that can be used to probe a compound library for inhibitors of auxin-induced Ca2+ entry in plant cell suspensions.
Keywords
Auxin signaling, Auxin-induced calcium signaling, Calcium signaling, Inhibitors

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MLA
De Vriese, Kjell, et al. “The Screening for Novel Inhibitors of Auxin-Induced Ca2+ Signaling.” Plant Chemical Genomics : Methods and Protocols, edited by Glenn R. Hicks and Chunhua Zhang, vol. 2213, Springer, 2021, pp. 89–98, doi:10.1007/978-1-0716-0954-5_8.
APA
De Vriese, K., Nguyen, L., Stael, S., Audenaert, D., Beeckman, T., & Vanneste, S. (2021). The screening for novel inhibitors of auxin-induced Ca2+ signaling. In G. R. Hicks & C. Zhang (Eds.), Plant chemical genomics : methods and protocols (Vol. 2213, pp. 89–98). Springer. https://doi.org/10.1007/978-1-0716-0954-5_8
Chicago author-date
De Vriese, Kjell, Long Nguyen, Simon Stael, Dominique Audenaert, Tom Beeckman, and Steffen Vanneste. 2021. “The Screening for Novel Inhibitors of Auxin-Induced Ca2+ Signaling.” In Plant Chemical Genomics : Methods and Protocols, edited by Glenn R. Hicks and Chunhua Zhang, 2213:89–98. Springer. https://doi.org/10.1007/978-1-0716-0954-5_8.
Chicago author-date (all authors)
De Vriese, Kjell, Long Nguyen, Simon Stael, Dominique Audenaert, Tom Beeckman, and Steffen Vanneste. 2021. “The Screening for Novel Inhibitors of Auxin-Induced Ca2+ Signaling.” In Plant Chemical Genomics : Methods and Protocols, ed by. Glenn R. Hicks and Chunhua Zhang, 2213:89–98. Springer. doi:10.1007/978-1-0716-0954-5_8.
Vancouver
1.
De Vriese K, Nguyen L, Stael S, Audenaert D, Beeckman T, Vanneste S. The screening for novel inhibitors of auxin-induced Ca2+ signaling. In: Hicks GR, Zhang C, editors. Plant chemical genomics : methods and protocols. Springer; 2021. p. 89–98.
IEEE
[1]
K. De Vriese, L. Nguyen, S. Stael, D. Audenaert, T. Beeckman, and S. Vanneste, “The screening for novel inhibitors of auxin-induced Ca2+ signaling,” in Plant chemical genomics : methods and protocols, vol. 2213, G. R. Hicks and C. Zhang, Eds. Springer, 2021, pp. 89–98.
@incollection{8683262,
  abstract     = {{Ca2+-based second messenger signaling is used by many signal perception mechanisms to modulate specific cellular responses. The well-characterized phytohormone auxin elicits a very rapid Ca2+ signal, but the molecular players involved in auxin-induced Ca2+ signaling are still largely unknown. The complicated and often redundant nature of the plant Ca2+ signaling machinery makes the use of mutants and transgenic lines a painstaking process, which makes a pharmacological approach an attractive alternative to study these processes. Here, we describe the development and utilization of a screening assay that can be used to probe a compound library for inhibitors of auxin-induced Ca2+ entry in plant cell suspensions.}},
  author       = {{De Vriese, Kjell and Nguyen, Long and Stael, Simon and Audenaert, Dominique and Beeckman, Tom and Vanneste, Steffen}},
  booktitle    = {{Plant chemical genomics : methods and protocols}},
  editor       = {{Hicks, Glenn R. and Zhang, Chunhua}},
  isbn         = {{9781071609538}},
  issn         = {{1064-3745}},
  keywords     = {{Auxin signaling,Auxin-induced calcium signaling,Calcium signaling,Inhibitors}},
  language     = {{eng}},
  pages        = {{89--98}},
  publisher    = {{Springer}},
  series       = {{Methods in Molecular Biology}},
  title        = {{The screening for novel inhibitors of auxin-induced Ca2+ signaling}},
  url          = {{http://dx.doi.org/10.1007/978-1-0716-0954-5_8}},
  volume       = {{2213}},
  year         = {{2021}},
}

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