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Synergistic antimicrobial activity of supplemented medical-grade honey against Pseudomonas aeruginosa biofilm formation and eradication

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Abstract
Biofilms hinder wound healing. Medical-grade honey (MGH) is a promising therapy because of its broad-spectrum antimicrobial activity and the lack of risk for resistance. This study investigated the inhibitory and eradicative activity against multidrug-resistant Pseudomonas aeruginosa biofilms by different established MGH-based wound care formulations. Six different natural wound care products (Medihoney, Revamil, Mebo, Melladerm, L-Mesitran Ointment, and L-Mesitran Soft) were tested in vitro. Most of them contain MGH only, whereas some were supplemented. L-Mesitran Soft demonstrated the most potent antimicrobial activity (6.08-log inhibition and 3.18-log eradication). Other formulations ranged between 0.89-log and 4.80-log inhibition and 0.65-log and 1.66-log eradication. Therefore, the contribution of different ingredients of L-Mesitran Soft was investigated in more detail. The activity of the same batch of raw MGH (1.38-log inhibition and 2.35-log eradication), vitamins C and E (0.95-log inhibition and 0.94-log eradication), and all ingredients except MGH (1.69-log inhibition and 0.75-log eradication) clearly support a synergistic activity of components within the L-Mesitran Soft formulation. Several presented clinical cases illustrate its clinical antimicrobial efficacy against Pseudomonas aeruginosa biofilms. In conclusion, MGH is a potent treatment for Pseudomonas biofilms. L-Mesitran Soft has the strongest antimicrobial activity, which is likely due to the synergistic activity mediated by its supplements.
Keywords
medical-grade honey, supplements, vitamin C, vitamin E, antimicrobial, biofilm, Pseudomonas aeruginosa, wound, BREAST-FEEDING WOMEN, MANUKA HONEY, VITAMIN-C, BACTERICIDAL ACTIVITY, PROPYLENE-GLYCOL, DAMAGED NIPPLES, ASCORBIC-ACID, ANIMAL-MODEL, WOUNDS, ANTIBACTERIAL

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MLA
Pleeging, Frans, et al. “Synergistic Antimicrobial Activity of Supplemented Medical-Grade Honey against Pseudomonas Aeruginosa Biofilm Formation and Eradication.” ANTIBIOTICS-BASEL, vol. 9, no. 12, 2020, doi:10.3390/antibiotics9120866.
APA
Pleeging, F., Coenye, T., Mossialos, D., De Rooster, H., Chrysostomou, D., Wagener, F. A. D. T. G., & Cremers, N. A. J. (2020). Synergistic antimicrobial activity of supplemented medical-grade honey against Pseudomonas aeruginosa biofilm formation and eradication. ANTIBIOTICS-BASEL, 9(12). https://doi.org/10.3390/antibiotics9120866
Chicago author-date
Pleeging, Frans, Tom Coenye, Dimitris Mossialos, Hilde De Rooster, Daniela Chrysostomou, Frank A. D. T. G. Wagener, and Niels A. J. Cremers. 2020. “Synergistic Antimicrobial Activity of Supplemented Medical-Grade Honey against Pseudomonas Aeruginosa Biofilm Formation and Eradication.” ANTIBIOTICS-BASEL 9 (12). https://doi.org/10.3390/antibiotics9120866.
Chicago author-date (all authors)
Pleeging, Frans, Tom Coenye, Dimitris Mossialos, Hilde De Rooster, Daniela Chrysostomou, Frank A. D. T. G. Wagener, and Niels A. J. Cremers. 2020. “Synergistic Antimicrobial Activity of Supplemented Medical-Grade Honey against Pseudomonas Aeruginosa Biofilm Formation and Eradication.” ANTIBIOTICS-BASEL 9 (12). doi:10.3390/antibiotics9120866.
Vancouver
1.
Pleeging F, Coenye T, Mossialos D, De Rooster H, Chrysostomou D, Wagener FADTG, et al. Synergistic antimicrobial activity of supplemented medical-grade honey against Pseudomonas aeruginosa biofilm formation and eradication. ANTIBIOTICS-BASEL. 2020;9(12).
IEEE
[1]
F. Pleeging et al., “Synergistic antimicrobial activity of supplemented medical-grade honey against Pseudomonas aeruginosa biofilm formation and eradication,” ANTIBIOTICS-BASEL, vol. 9, no. 12, 2020.
@article{8683007,
  abstract     = {{Biofilms hinder wound healing. Medical-grade honey (MGH) is a promising therapy because of its broad-spectrum antimicrobial activity and the lack of risk for resistance. This study investigated the inhibitory and eradicative activity against multidrug-resistant Pseudomonas aeruginosa biofilms by different established MGH-based wound care formulations. Six different natural wound care products (Medihoney, Revamil, Mebo, Melladerm, L-Mesitran Ointment, and L-Mesitran Soft) were tested in vitro. Most of them contain MGH only, whereas some were supplemented. L-Mesitran Soft demonstrated the most potent antimicrobial activity (6.08-log inhibition and 3.18-log eradication). Other formulations ranged between 0.89-log and 4.80-log inhibition and 0.65-log and 1.66-log eradication. Therefore, the contribution of different ingredients of L-Mesitran Soft was investigated in more detail. The activity of the same batch of raw MGH (1.38-log inhibition and 2.35-log eradication), vitamins C and E (0.95-log inhibition and 0.94-log eradication), and all ingredients except MGH (1.69-log inhibition and 0.75-log eradication) clearly support a synergistic activity of components within the L-Mesitran Soft formulation. Several presented clinical cases illustrate its clinical antimicrobial efficacy against Pseudomonas aeruginosa biofilms. In conclusion, MGH is a potent treatment for Pseudomonas biofilms. L-Mesitran Soft has the strongest antimicrobial activity, which is likely due to the synergistic activity mediated by its supplements.}},
  articleno    = {{866}},
  author       = {{Pleeging, Frans and Coenye, Tom and Mossialos, Dimitris and De Rooster, Hilde and Chrysostomou, Daniela and Wagener, Frank A. D. T. G. and Cremers, Niels A. J.}},
  issn         = {{2079-6382}},
  journal      = {{ANTIBIOTICS-BASEL}},
  keywords     = {{medical-grade honey,supplements,vitamin C,vitamin E,antimicrobial,biofilm,Pseudomonas aeruginosa,wound,BREAST-FEEDING WOMEN,MANUKA HONEY,VITAMIN-C,BACTERICIDAL ACTIVITY,PROPYLENE-GLYCOL,DAMAGED NIPPLES,ASCORBIC-ACID,ANIMAL-MODEL,WOUNDS,ANTIBACTERIAL}},
  language     = {{eng}},
  number       = {{12}},
  pages        = {{16}},
  title        = {{Synergistic antimicrobial activity of supplemented medical-grade honey against Pseudomonas aeruginosa biofilm formation and eradication}},
  url          = {{http://dx.doi.org/10.3390/antibiotics9120866}},
  volume       = {{9}},
  year         = {{2020}},
}

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