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Pathogenic potential of Escherichia coli O157 and O26 isolated from young Belgian dairy calves by recto-anal mucosal swab culturing

(2021) JOURNAL OF APPLIED MICROBIOLOGY. 131(2). p.964-972
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Abstract
Aims: The purpose of this study was to investigate the occurrence of E. coli O157 and O26 on Belgian dairy cattle farms, the presence of virulence genes in the confirmed isolates, and the association of E. coli O26 presence with calf diarrhea. Methods and Results: In total, 233 recto‐anal mucosal swabs (RAMS) were obtained from healthy and diarrheic dairy calves on three farms, each alternately visited three consecutive times. RAMS were analyzed for presence of E. coli O157 and O26, and stx1, stx2 and eae virulence genes. Overall, 19% of RAMS tested positive for E. coli O157, while 31% tested positive for E. coli O26. The majority of isolates possessed both stx and eae, denoting a high pathogenic potential to humans. While both serogroups persisted at farm level, persistence within the same animal over time appeared to be relatively rare. Interestingly, E. coli O26 was already abundantly present at a younger age compared to E. coli O157. Calf diarrhea could not be associated with presence of E. coli O26. Conclusions: Young dairy calves are important on‐farm reservoirs of potentially pathogenic E. coli O157 and O26. A role of E. coli O26 in calf diarrhea could not be confirmed. Significance and Impact of Study: O157 and O26 are responsible for the majority of human STEC infections. Gaining more epidemiological information regarding their occurrence and persistence on cattle farms will contribute to a better understanding of STEC ecology and risk of human transmission.
Keywords
Biotechnology, Applied Microbiology and Biotechnology, General Medicine, Belgium, dairy cattle, Escherichia coli, O157, O26, persistence, recto&#8208, anal mucosal swab, CATTLE, BOVINE, STEC, IDENTIFICATION, COLONIZATION, PREVALENCE, INFECTION, HUMANS

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Citation

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MLA
Engelen, Frederik, et al. “Pathogenic Potential of Escherichia Coli O157 and O26 Isolated from Young Belgian Dairy Calves by Recto-Anal Mucosal Swab Culturing.” JOURNAL OF APPLIED MICROBIOLOGY, vol. 131, no. 2, 2021, pp. 964–72, doi:10.1111/jam.14909.
APA
Engelen, F., Thiry, D., Devleesschauwer, B., Heyndrickx, M., Mainil, J., De Zutter, L., & Cox, E. (2021). Pathogenic potential of Escherichia coli O157 and O26 isolated from young Belgian dairy calves by recto-anal mucosal swab culturing. JOURNAL OF APPLIED MICROBIOLOGY, 131(2), 964–972. https://doi.org/10.1111/jam.14909
Chicago author-date
Engelen, Frederik, Damien Thiry, Brecht Devleesschauwer, Marc Heyndrickx, Jacques Mainil, Lieven De Zutter, and Eric Cox. 2021. “Pathogenic Potential of Escherichia Coli O157 and O26 Isolated from Young Belgian Dairy Calves by Recto-Anal Mucosal Swab Culturing.” JOURNAL OF APPLIED MICROBIOLOGY 131 (2): 964–72. https://doi.org/10.1111/jam.14909.
Chicago author-date (all authors)
Engelen, Frederik, Damien Thiry, Brecht Devleesschauwer, Marc Heyndrickx, Jacques Mainil, Lieven De Zutter, and Eric Cox. 2021. “Pathogenic Potential of Escherichia Coli O157 and O26 Isolated from Young Belgian Dairy Calves by Recto-Anal Mucosal Swab Culturing.” JOURNAL OF APPLIED MICROBIOLOGY 131 (2): 964–972. doi:10.1111/jam.14909.
Vancouver
1.
Engelen F, Thiry D, Devleesschauwer B, Heyndrickx M, Mainil J, De Zutter L, et al. Pathogenic potential of Escherichia coli O157 and O26 isolated from young Belgian dairy calves by recto-anal mucosal swab culturing. JOURNAL OF APPLIED MICROBIOLOGY. 2021;131(2):964–72.
IEEE
[1]
F. Engelen et al., “Pathogenic potential of Escherichia coli O157 and O26 isolated from young Belgian dairy calves by recto-anal mucosal swab culturing,” JOURNAL OF APPLIED MICROBIOLOGY, vol. 131, no. 2, pp. 964–972, 2021.
@article{8682180,
  abstract     = {{Aims: The purpose of this study was to investigate the occurrence of E. coli O157 and O26 on Belgian dairy cattle farms, the presence of virulence genes in the confirmed isolates, and the association of E. coli O26 presence with calf diarrhea.

Methods and Results: In total, 233 recto‐anal mucosal swabs (RAMS) were obtained from healthy and diarrheic dairy calves on three farms, each alternately visited three consecutive times. RAMS were analyzed for presence of E. coli O157 and O26, and stx1, stx2 and eae virulence genes. Overall, 19% of RAMS tested positive for E. coli O157, while 31% tested positive for E. coli O26. The majority of isolates possessed both stx and eae, denoting a high pathogenic potential to humans. While both serogroups persisted at farm level, persistence within the same animal over time appeared to be relatively rare. Interestingly, E. coli O26 was already abundantly present at a younger age compared to E. coli O157. Calf diarrhea could not be associated with presence of E. coli O26.

Conclusions: Young dairy calves are important on‐farm reservoirs of potentially pathogenic E. coli O157 and O26. A role of E. coli O26 in calf diarrhea could not be confirmed.

Significance and Impact of Study: O157 and O26 are responsible for the majority of human STEC infections. Gaining more epidemiological information regarding their occurrence and persistence on cattle farms will contribute to a better understanding of STEC ecology and risk of human transmission.}},
  author       = {{Engelen, Frederik and Thiry, Damien and Devleesschauwer, Brecht and Heyndrickx, Marc and Mainil, Jacques and De Zutter, Lieven and Cox, Eric}},
  issn         = {{1364-5072}},
  journal      = {{JOURNAL OF APPLIED MICROBIOLOGY}},
  keywords     = {{Biotechnology,Applied Microbiology and Biotechnology,General Medicine,Belgium,dairy cattle,Escherichia coli,O157,O26,persistence,recto&#8208,anal mucosal swab,CATTLE,BOVINE,STEC,IDENTIFICATION,COLONIZATION,PREVALENCE,INFECTION,HUMANS}},
  language     = {{eng}},
  number       = {{2}},
  pages        = {{964--972}},
  title        = {{Pathogenic potential of Escherichia coli O157 and O26 isolated from young Belgian dairy calves by recto-anal mucosal swab culturing}},
  url          = {{http://dx.doi.org/10.1111/jam.14909}},
  volume       = {{131}},
  year         = {{2021}},
}

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