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Identification and characterization of striking multiple-insecticide resistance in a Tetranychus urticae field population from Greece

(2021) PEST MANAGEMENT SCIENCE. 77(2). p.666-676
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  • POLYADAPT (Molecular-genetic mechanisms of extreme adaptation in a polyphagous agricultural pest)
Abstract
BACKGROUND Tetranychus urticae is a notorious crop pest with a worldwide distribution that has developed resistance to a wide range of acaricides. Here, we investigated the resistance levels of a T. urticae population collected from an ornamental greenhouse in Peloponnese, Greece, and analyzed its resistance mechanisms at the molecular level. RESULTS Toxicological assays showed resistance against compounds with different modes of action, with resistance ratios of: 89-fold for abamectin; > 1000-fold for clofentezine; > 5000-fold for etoxazole; 27-fold for fenpyroximate and pyridaben; 20- and 36-fold for spirodiclofen and spirotetramat, respectively; and 116- and > 500-fold for cyenopyrafen and cyflumetofen, respectively. Bioassays with synergists indicated the involvement of detoxification enzymes in resistance to abamectin, but not to cyflumetofen and spirodiclofen. RNA sequencing (RNA-seq) analysis showed significant over-expression of several genes encoding detoxification enzymes such as cytochrome P450 monooxygenases and UDP-glycosyltransferases, which have been previously associated with acaricide resistance. Known target-site resistance mutations were identified in acetyl-choline esterase, chitin synthase 1 and NDUFS7/psst, but putative novel resistance mutations were also discovered in targets such as glutamate-gated chloride channel subunit 3. Interestingly, target-site resistance mutations against pyrethroids or bifenazate were not identified, possibly indicating a recent reduced selection pressure in Greece, as well as a possible opportunity to rotate these chemistries. CONCLUSION We identified and characterized a striking case of multiple acaricide resistance in a field population of T. urticae. Exceptionally strong resistance phenotypes, with accumulation of multiple resistance mutations and over-expression of P450s and other detoxification genes in the same field population are reported.
Keywords
two-spotted spider mite, transcriptomics, mutations, acaricide, multi-resistance, 2-SPOTTED SPIDER-MITE, GATED CHLORIDE CHANNEL, TARGET-SITE RESISTANCE, ACARICIDE RESISTANCE, ABAMECTIN RESISTANCE, KOCH ACARI, CROSS-RESISTANCE, PLUTELLA-XYLOSTELLA, MOLECULAR ANALYSIS, GROWTH-INHIBITORS

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Citation

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MLA
Papapostolou, Kyriaki Maria, et al. “Identification and Characterization of Striking Multiple-Insecticide Resistance in a Tetranychus Urticae Field Population from Greece.” PEST MANAGEMENT SCIENCE, vol. 77, no. 2, 2021, pp. 666–76, doi:10.1002/ps.6136.
APA
Papapostolou, K. M., Riga, M., Charamis, J., Skoufa, E., Souchlas, V., Ilias, A., … Vontas, J. (2021). Identification and characterization of striking multiple-insecticide resistance in a Tetranychus urticae field population from Greece. PEST MANAGEMENT SCIENCE, 77(2), 666–676. https://doi.org/10.1002/ps.6136
Chicago author-date
Papapostolou, Kyriaki Maria, Maria Riga, Jason Charamis, Evangelia Skoufa, Vassilis Souchlas, Aris Ilias, Wannes Dermauw, Panagiotis Ioannidis, Thomas Van Leeuwen, and John Vontas. 2021. “Identification and Characterization of Striking Multiple-Insecticide Resistance in a Tetranychus Urticae Field Population from Greece.” PEST MANAGEMENT SCIENCE 77 (2): 666–76. https://doi.org/10.1002/ps.6136.
Chicago author-date (all authors)
Papapostolou, Kyriaki Maria, Maria Riga, Jason Charamis, Evangelia Skoufa, Vassilis Souchlas, Aris Ilias, Wannes Dermauw, Panagiotis Ioannidis, Thomas Van Leeuwen, and John Vontas. 2021. “Identification and Characterization of Striking Multiple-Insecticide Resistance in a Tetranychus Urticae Field Population from Greece.” PEST MANAGEMENT SCIENCE 77 (2): 666–676. doi:10.1002/ps.6136.
Vancouver
1.
Papapostolou KM, Riga M, Charamis J, Skoufa E, Souchlas V, Ilias A, et al. Identification and characterization of striking multiple-insecticide resistance in a Tetranychus urticae field population from Greece. PEST MANAGEMENT SCIENCE. 2021;77(2):666–76.
IEEE
[1]
K. M. Papapostolou et al., “Identification and characterization of striking multiple-insecticide resistance in a Tetranychus urticae field population from Greece,” PEST MANAGEMENT SCIENCE, vol. 77, no. 2, pp. 666–676, 2021.
@article{8681840,
  abstract     = {{BACKGROUND Tetranychus urticae is a notorious crop pest with a worldwide distribution that has developed resistance to a wide range of acaricides. Here, we investigated the resistance levels of a T. urticae population collected from an ornamental greenhouse in Peloponnese, Greece, and analyzed its resistance mechanisms at the molecular level.

RESULTS Toxicological assays showed resistance against compounds with different modes of action, with resistance ratios of: 89-fold for abamectin; > 1000-fold for clofentezine; > 5000-fold for etoxazole; 27-fold for fenpyroximate and pyridaben; 20- and 36-fold for spirodiclofen and spirotetramat, respectively; and 116- and > 500-fold for cyenopyrafen and cyflumetofen, respectively.

Bioassays with synergists indicated the involvement of detoxification enzymes in resistance to abamectin, but not to cyflumetofen and spirodiclofen. RNA sequencing (RNA-seq) analysis showed significant over-expression of several genes encoding detoxification enzymes such as cytochrome P450 monooxygenases and UDP-glycosyltransferases, which have been previously associated with acaricide resistance. Known target-site resistance mutations were identified in acetyl-choline esterase, chitin synthase 1 and NDUFS7/psst, but putative novel resistance mutations were also discovered in targets such as glutamate-gated chloride channel subunit 3. Interestingly, target-site resistance mutations against pyrethroids or bifenazate were not identified, possibly indicating a recent reduced selection pressure in Greece, as well as a possible opportunity to rotate these chemistries.

CONCLUSION We identified and characterized a striking case of multiple acaricide resistance in a field population of T. urticae. Exceptionally strong resistance phenotypes, with accumulation of multiple resistance mutations and over-expression of P450s and other detoxification genes in the same field population are reported.}},
  author       = {{Papapostolou, Kyriaki Maria and Riga, Maria and Charamis, Jason and Skoufa, Evangelia and Souchlas, Vassilis and Ilias, Aris and Dermauw, Wannes and Ioannidis, Panagiotis and Van Leeuwen, Thomas and Vontas, John}},
  issn         = {{1526-498X}},
  journal      = {{PEST MANAGEMENT SCIENCE}},
  keywords     = {{two-spotted spider mite,transcriptomics,mutations,acaricide,multi-resistance,2-SPOTTED SPIDER-MITE,GATED CHLORIDE CHANNEL,TARGET-SITE RESISTANCE,ACARICIDE RESISTANCE,ABAMECTIN RESISTANCE,KOCH ACARI,CROSS-RESISTANCE,PLUTELLA-XYLOSTELLA,MOLECULAR ANALYSIS,GROWTH-INHIBITORS}},
  language     = {{eng}},
  number       = {{2}},
  pages        = {{666--676}},
  title        = {{Identification and characterization of striking multiple-insecticide resistance in a Tetranychus urticae field population from Greece}},
  url          = {{http://dx.doi.org/10.1002/ps.6136}},
  volume       = {{77}},
  year         = {{2021}},
}

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