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Designer Descemet membranes containing PDLLA and functionalized gelatins as corneal endothelial scaffold

Jasper Van Hoorick (UGent) , Jasper Delaey, Hendrik Vercammen, Jurgen Van Erps, Hugo Thienpont (UGent) , Peter Dubruel (UGent) , Nadia Zakaria, Carina Koppen, Sandra Van Vlierberghe (UGent) and Bert van den Bogerd
(2020) ADVANCED HEALTHCARE MATERIALS. 9(16).
Author
Organization
Abstract
Corneal blindness is the fourth leading cause of visual impairment. Of specific interest is blindness due to a dysfunctional corneal endothelium which can only be treated by transplanting healthy tissue from a deceased donor. Unfortunately, corneal supply does not meet the demand with only one donor for every 70 patients. Therefore, there is a huge interest in tissue engineering of grafts consisting of an ultra-thin scaffold seeded with cultured endothelial cells. The present research describes the fabrication of such artificial Descemet membranes based on the combination of a biodegradable amorphous polyester (poly (d,l-lactic acid)) and crosslinkable gelatins. Four different crosslinkable gelatin derivatives are compared in terms of processing, membrane quality, and function, as well as biological performance in the presence of corneal endothelial cells. The membranes are fabricated through multi-step spincoating, including a sacrificial layer to allow for straightforward membrane detachment after production. As a consequence, ultrathin (<1 mu m), highly transparent (>90%), semi-permeable membranes could be obtained with high biological potential. The membranes supported the characteristic morphology and correct phenotype of corneal endothelial cells while exhibiting similar proliferation rates as the positive control. As a consequence, the proposed membranes prove to be a promising synthetic alternative to donor tissue.
Keywords
MECHANICAL-PROPERTIES, TRANSPLANTATION, SUBSTRATE, POLYMERIZATION, POLYESTERS, INHIBITOR, HYDROGELS, STIFFNESS, SURFACE, LACTIDE, corneal endothelium, gelatin norbornene, gelatin-methacryloyl, poly(D L) lactic acid, tissue engineering

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MLA
Van Hoorick, Jasper, et al. “Designer Descemet Membranes Containing PDLLA and Functionalized Gelatins as Corneal Endothelial Scaffold.” ADVANCED HEALTHCARE MATERIALS, vol. 9, no. 16, 2020, doi:10.1002/adhm.202000760.
APA
Van Hoorick, J., Delaey, J., Vercammen, H., Van Erps, J., Thienpont, H., Dubruel, P., … van den Bogerd, B. (2020). Designer Descemet membranes containing PDLLA and functionalized gelatins as corneal endothelial scaffold. ADVANCED HEALTHCARE MATERIALS, 9(16). https://doi.org/10.1002/adhm.202000760
Chicago author-date
Van Hoorick, Jasper, Jasper Delaey, Hendrik Vercammen, Jurgen Van Erps, Hugo Thienpont, Peter Dubruel, Nadia Zakaria, Carina Koppen, Sandra Van Vlierberghe, and Bert van den Bogerd. 2020. “Designer Descemet Membranes Containing PDLLA and Functionalized Gelatins as Corneal Endothelial Scaffold.” ADVANCED HEALTHCARE MATERIALS 9 (16). https://doi.org/10.1002/adhm.202000760.
Chicago author-date (all authors)
Van Hoorick, Jasper, Jasper Delaey, Hendrik Vercammen, Jurgen Van Erps, Hugo Thienpont, Peter Dubruel, Nadia Zakaria, Carina Koppen, Sandra Van Vlierberghe, and Bert van den Bogerd. 2020. “Designer Descemet Membranes Containing PDLLA and Functionalized Gelatins as Corneal Endothelial Scaffold.” ADVANCED HEALTHCARE MATERIALS 9 (16). doi:10.1002/adhm.202000760.
Vancouver
1.
Van Hoorick J, Delaey J, Vercammen H, Van Erps J, Thienpont H, Dubruel P, et al. Designer Descemet membranes containing PDLLA and functionalized gelatins as corneal endothelial scaffold. ADVANCED HEALTHCARE MATERIALS. 2020;9(16).
IEEE
[1]
J. Van Hoorick et al., “Designer Descemet membranes containing PDLLA and functionalized gelatins as corneal endothelial scaffold,” ADVANCED HEALTHCARE MATERIALS, vol. 9, no. 16, 2020.
@article{8680225,
  abstract     = {{Corneal blindness is the fourth leading cause of visual impairment. Of specific interest is blindness due to a dysfunctional corneal endothelium which can only be treated by transplanting healthy tissue from a deceased donor. Unfortunately, corneal supply does not meet the demand with only one donor for every 70 patients. Therefore, there is a huge interest in tissue engineering of grafts consisting of an ultra-thin scaffold seeded with cultured endothelial cells. The present research describes the fabrication of such artificial Descemet membranes based on the combination of a biodegradable amorphous polyester (poly (d,l-lactic acid)) and crosslinkable gelatins. Four different crosslinkable gelatin derivatives are compared in terms of processing, membrane quality, and function, as well as biological performance in the presence of corneal endothelial cells. The membranes are fabricated through multi-step spincoating, including a sacrificial layer to allow for straightforward membrane detachment after production. As a consequence, ultrathin (<1 mu m), highly transparent (>90%), semi-permeable membranes could be obtained with high biological potential. The membranes supported the characteristic morphology and correct phenotype of corneal endothelial cells while exhibiting similar proliferation rates as the positive control. As a consequence, the proposed membranes prove to be a promising synthetic alternative to donor tissue.}},
  articleno    = {{2000760}},
  author       = {{Van Hoorick, Jasper and Delaey, Jasper and Vercammen, Hendrik and Van Erps, Jurgen and Thienpont, Hugo and Dubruel, Peter and Zakaria, Nadia and Koppen, Carina and Van Vlierberghe, Sandra and van den Bogerd, Bert}},
  issn         = {{2192-2640}},
  journal      = {{ADVANCED HEALTHCARE MATERIALS}},
  keywords     = {{MECHANICAL-PROPERTIES,TRANSPLANTATION,SUBSTRATE,POLYMERIZATION,POLYESTERS,INHIBITOR,HYDROGELS,STIFFNESS,SURFACE,LACTIDE,corneal endothelium,gelatin norbornene,gelatin-methacryloyl,poly(D L) lactic acid,tissue engineering}},
  language     = {{eng}},
  number       = {{16}},
  pages        = {{16}},
  title        = {{Designer Descemet membranes containing PDLLA and functionalized gelatins as corneal endothelial scaffold}},
  url          = {{http://doi.org/10.1002/adhm.202000760}},
  volume       = {{9}},
  year         = {{2020}},
}
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