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Enterobactin deficiency in a coliform mastitis isolate decreases its fitness in a murine model : a preliminary host-pathogen interaction study

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Abstract
Iron is an essential nutrient for bacterial growth. Therefore, bacteria have evolved chelation mechanisms to acquire iron for their survival. Enterobactin, a chelator with high affinity for ferric iron, is secreted by Escherichia coli and contributes to its improved bacterial fitness. In this preliminary study, we evaluated enterobactin deficiency both in vitro and in vivo in the context of E. coli mastitis. Firstly, we showed that expression of lipocalin 2, a protein produced by the host that is able to both bind and deplete enterobactin, is increased upon E. coli infection in the cow’s mastitic mammary gland. Secondly, we demonstrated in vitro that enterobactin deficiency does not alter interleukin (IL)-8 expression in bovine mammary epithelial cells and its associated neutrophil recruitment. However, a significantly increased reactive oxygen species production of these neutrophils was observed. Thirdly, we showed there was no significant difference in bacterial in vitro growth between the enterobactin-deficient mutant and its wild-type counterpart. However, when further explored in a murine model for bovine mastitis, the enterobactin-deficient mutant vs. the wild-type strain revealed a significant reduction of the bacterial load and, consequently, a decrease in pro-inflammatory cytokines (IL-1a,−1b,−4,−6, and−8). A reduced neutrophilic influx was also observed immunohistochemically. These findings therefore identify interference of the enterobactin iron-scavenging mechanism as a potential measure to decrease the fitness of E. coli in the mastitic mammary gland.
Keywords
E. coli mastitis, enterobactin, iron scavenging, siderophores, lipocalin 2 (also known as NGAL), MAMMARY EPITHELIAL-CELLS, CLINICAL MASTITIS, ANTIMICROBIAL SUSCEPTIBILITY, IRON-METABOLISM, DAIRY-COWS, INTERLEUKIN-8, INFECTION

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MLA
Vander Elst, Niels, et al. “Enterobactin Deficiency in a Coliform Mastitis Isolate Decreases Its Fitness in a Murine Model : A Preliminary Host-Pathogen Interaction Study.” FRONTIERS IN VETERINARY SCIENCE, vol. 7, 2020, doi:10.3389/fvets.2020.576583.
APA
Vander Elst, N., Breyne, K., Steenbrugge, J., Gibson, A. J., Smith, D. G. E., Germon, P., … Meyer, E. (2020). Enterobactin deficiency in a coliform mastitis isolate decreases its fitness in a murine model : a preliminary host-pathogen interaction study. FRONTIERS IN VETERINARY SCIENCE, 7. https://doi.org/10.3389/fvets.2020.576583
Chicago author-date
Vander Elst, Niels, Koen Breyne, Jonas Steenbrugge, Amanda Jane Gibson, David George Emslie Smith, Pierre Germon, Dirk Werling, and Evelyne Meyer. 2020. “Enterobactin Deficiency in a Coliform Mastitis Isolate Decreases Its Fitness in a Murine Model : A Preliminary Host-Pathogen Interaction Study.” FRONTIERS IN VETERINARY SCIENCE 7. https://doi.org/10.3389/fvets.2020.576583.
Chicago author-date (all authors)
Vander Elst, Niels, Koen Breyne, Jonas Steenbrugge, Amanda Jane Gibson, David George Emslie Smith, Pierre Germon, Dirk Werling, and Evelyne Meyer. 2020. “Enterobactin Deficiency in a Coliform Mastitis Isolate Decreases Its Fitness in a Murine Model : A Preliminary Host-Pathogen Interaction Study.” FRONTIERS IN VETERINARY SCIENCE 7. doi:10.3389/fvets.2020.576583.
Vancouver
1.
Vander Elst N, Breyne K, Steenbrugge J, Gibson AJ, Smith DGE, Germon P, et al. Enterobactin deficiency in a coliform mastitis isolate decreases its fitness in a murine model : a preliminary host-pathogen interaction study. FRONTIERS IN VETERINARY SCIENCE. 2020;7.
IEEE
[1]
N. Vander Elst et al., “Enterobactin deficiency in a coliform mastitis isolate decreases its fitness in a murine model : a preliminary host-pathogen interaction study,” FRONTIERS IN VETERINARY SCIENCE, vol. 7, 2020.
@article{8680091,
  abstract     = {{Iron is an essential nutrient for bacterial growth. Therefore, bacteria have evolved chelation mechanisms to acquire iron for their survival. Enterobactin, a chelator with high affinity for ferric iron, is secreted by Escherichia coli and contributes to its improved bacterial fitness. In this preliminary study, we evaluated enterobactin deficiency both in vitro and in vivo in the context of E. coli mastitis. Firstly, we showed that expression of lipocalin 2, a protein produced by the host that is able to both bind and deplete enterobactin, is increased upon E. coli infection in the cow’s mastitic mammary gland. Secondly, we demonstrated in vitro that enterobactin deficiency does not alter interleukin (IL)-8 expression in bovine mammary epithelial cells and its associated neutrophil recruitment. However, a significantly increased reactive oxygen
species production of these neutrophils was observed. Thirdly, we showed there was no significant difference in bacterial in vitro growth between the enterobactin-deficient mutant and its wild-type counterpart. However, when further explored in a murine model for bovine mastitis, the enterobactin-deficient mutant vs. the wild-type strain revealed a significant reduction of the bacterial load and, consequently, a decrease in pro-inflammatory cytokines (IL-1a,−1b,−4,−6, and−8). A reduced neutrophilic influx was also observed immunohistochemically. These findings therefore identify interference of the enterobactin iron-scavenging mechanism as a potential measure to decrease the fitness of E. coli in the mastitic mammary gland.}},
  articleno    = {{576583}},
  author       = {{Vander Elst, Niels and Breyne, Koen and Steenbrugge, Jonas and Gibson, Amanda Jane and Smith, David George Emslie and Germon, Pierre and Werling, Dirk and Meyer, Evelyne}},
  issn         = {{2297-1769}},
  journal      = {{FRONTIERS IN VETERINARY SCIENCE}},
  keywords     = {{E. coli mastitis,enterobactin,iron scavenging,siderophores,lipocalin 2 (also known as NGAL),MAMMARY EPITHELIAL-CELLS,CLINICAL MASTITIS,ANTIMICROBIAL SUSCEPTIBILITY,IRON-METABOLISM,DAIRY-COWS,INTERLEUKIN-8,INFECTION}},
  language     = {{eng}},
  pages        = {{11}},
  title        = {{Enterobactin deficiency in a coliform mastitis isolate decreases its fitness in a murine model : a preliminary host-pathogen interaction study}},
  url          = {{http://dx.doi.org/10.3389/fvets.2020.576583}},
  volume       = {{7}},
  year         = {{2020}},
}

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