Effects of meta-topolin derivatives and temporary immersion on hyperhydricity and in vitro shoot proliferation in Pyrus communis
- Author
- Mariem Lotfi (UGent) , Chokri Bayoudh, Stefaan Werbrouck (UGent) and Messaoud Mars
- Organization
- Abstract
- Although micropropagation in temporary immersion systems might increase plant growth and multiplication, it can also cause specific problems such as hyperhydricity and losses by contamination. A new commercial temporary immersion bioreactor, SETIS™, was used to micropropagate two Tunisian pear cultivars, ‘Arbi’ and ‘Mahdia 6’. The latter cultivar was endogenously contaminated by Sphingomonas. Hyperhydricity was inevitable when 5 µM benzyladenine was applied. However, the symptoms could be reduced by lowering the immersion frequency to 3 times per day. Applying 5 µM meta–Methoxy topolin riboside (MemTR) or meta–Topolin riboside (mTR) completely inhibited hyperhydric shoot formation. Moreover, the addition of Plant Preservative Mixture was effective to control Sphingomonas and allowed the plants to proliferate. For both pear cultivars, the highest number of shoots per explant was induced by 5 µM MemTR, whereas the highest leaf area was obtained with 5 µM mTR. The longest shoots were obtained with 5 µM mTR for ‘Arbi’ and 5 µM MemTR for ‘Mahdia 6’. Key message Methoxy topoline-riboside (MemTR) and meta-topoline-riboside (mTR) were used as alternatives to benzyladenine to prevent hyperhydricity during the micropropagation of pears in a new temporary immersion system. These cytokinins also increased the number of good quality shoots, characterized by large leaves and longer shoots.
- Keywords
- Horticulture, Cytokinins, Hyperhydricity, Temporary, Immersion, Bioreactor, Contamination, META-TOPOLIN, AROMATIC CYTOKININS, LIQUID-MEDIUM, MICROPROPAGATION, BIOREACTOR, GROWTH, L.
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-8679558
- MLA
- Lotfi, Mariem, et al. “Effects of Meta-Topolin Derivatives and Temporary Immersion on Hyperhydricity and in Vitro Shoot Proliferation in Pyrus Communis.” PLANT CELL TISSUE AND ORGAN CULTURE, vol. 143, no. 3, 2020, pp. 499–505, doi:10.1007/s11240-020-01935-x.
- APA
- Lotfi, M., Bayoudh, C., Werbrouck, S., & Mars, M. (2020). Effects of meta-topolin derivatives and temporary immersion on hyperhydricity and in vitro shoot proliferation in Pyrus communis. PLANT CELL TISSUE AND ORGAN CULTURE, 143(3), 499–505. https://doi.org/10.1007/s11240-020-01935-x
- Chicago author-date
- Lotfi, Mariem, Chokri Bayoudh, Stefaan Werbrouck, and Messaoud Mars. 2020. “Effects of Meta-Topolin Derivatives and Temporary Immersion on Hyperhydricity and in Vitro Shoot Proliferation in Pyrus Communis.” PLANT CELL TISSUE AND ORGAN CULTURE 143 (3): 499–505. https://doi.org/10.1007/s11240-020-01935-x.
- Chicago author-date (all authors)
- Lotfi, Mariem, Chokri Bayoudh, Stefaan Werbrouck, and Messaoud Mars. 2020. “Effects of Meta-Topolin Derivatives and Temporary Immersion on Hyperhydricity and in Vitro Shoot Proliferation in Pyrus Communis.” PLANT CELL TISSUE AND ORGAN CULTURE 143 (3): 499–505. doi:10.1007/s11240-020-01935-x.
- Vancouver
- 1.Lotfi M, Bayoudh C, Werbrouck S, Mars M. Effects of meta-topolin derivatives and temporary immersion on hyperhydricity and in vitro shoot proliferation in Pyrus communis. PLANT CELL TISSUE AND ORGAN CULTURE. 2020;143(3):499–505.
- IEEE
- [1]M. Lotfi, C. Bayoudh, S. Werbrouck, and M. Mars, “Effects of meta-topolin derivatives and temporary immersion on hyperhydricity and in vitro shoot proliferation in Pyrus communis,” PLANT CELL TISSUE AND ORGAN CULTURE, vol. 143, no. 3, pp. 499–505, 2020.
@article{8679558,
abstract = {{Although micropropagation in temporary immersion systems might increase plant growth and multiplication, it can also cause specific problems such as hyperhydricity and losses by contamination. A new commercial temporary immersion bioreactor, SETIS™, was used to micropropagate two Tunisian pear cultivars, ‘Arbi’ and ‘Mahdia 6’. The latter cultivar was endogenously contaminated by Sphingomonas. Hyperhydricity was inevitable when 5 µM benzyladenine was applied. However, the symptoms could be reduced by lowering the immersion frequency to 3 times per day. Applying 5 µM meta–Methoxy topolin riboside (MemTR) or meta–Topolin riboside (mTR) completely inhibited hyperhydric shoot formation. Moreover, the addition of Plant Preservative Mixture was effective to control Sphingomonas and allowed the plants to proliferate. For both pear cultivars, the highest number of shoots per explant was induced by 5 µM MemTR, whereas the highest leaf area was obtained with 5 µM mTR. The longest shoots were obtained with 5 µM mTR for ‘Arbi’ and 5 µM MemTR for ‘Mahdia 6’.
Key message
Methoxy topoline-riboside (MemTR) and meta-topoline-riboside (mTR) were used as alternatives to benzyladenine to prevent hyperhydricity during the micropropagation of pears in a new temporary immersion system. These cytokinins also increased the number of good quality shoots, characterized by large leaves and longer shoots.}},
author = {{Lotfi, Mariem and Bayoudh, Chokri and Werbrouck, Stefaan and Mars, Messaoud}},
issn = {{0167-6857}},
journal = {{PLANT CELL TISSUE AND ORGAN CULTURE}},
keywords = {{Horticulture,Cytokinins,Hyperhydricity,Temporary,Immersion,Bioreactor,Contamination,META-TOPOLIN,AROMATIC CYTOKININS,LIQUID-MEDIUM,MICROPROPAGATION,BIOREACTOR,GROWTH,L.}},
language = {{eng}},
number = {{3}},
pages = {{499--505}},
title = {{Effects of meta-topolin derivatives and temporary immersion on hyperhydricity and in vitro shoot proliferation in Pyrus communis}},
url = {{http://doi.org/10.1007/s11240-020-01935-x}},
volume = {{143}},
year = {{2020}},
}
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