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Microbial enrichment, functional characterization and isolation from a cold seep yield piezotolerant obligate hydrocarbon degraders

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Abstract
Deep-sea environments can become contaminated with petroleum hydrocarbons. The effects of hydrostatic pressure (HP) in the deep sea on microbial oil degradation are poorly understood. Here, we performed long-term enrichments (100 days) from a natural cold seep while providing optimal conditions to sustain high hydrocarbon degradation rates. Through enrichments performed at increased HP and ambient pressure (AP) and by using control enrichments with marine broth, we demonstrated that both pressure and carbon source can have a big impact on the community structure. In contrast to previous studies, hydrocarbonoclastic operational taxonomic units (OTUs) remained dominant at both AP and increased HP, suggesting piezotolerance of these OTUs over the tested pressure range. Twenty-three isolates were obtained after isolation and dereplication. After recultivation at increased HP, an Alcanivorax sp. showed promising piezotolerance in axenic culture. Furthermore, preliminary co-cultivation tests indicated synergistic growth between some isolates, which shows promise for future synthetic community construction. Overall, more insights into the effect of increased HP on oil-degrading communities were obtained as well as several interesting isolates, e.g. a piezotolerant hydrocarbonoclastic bacterium for future deep-sea bioaugmentation investigation.
Keywords
bioaugmentation, oil degradation, microbial community, hydrocarbonoclastic, piezotolerance, deep-sea bacteria, HIGH HYDROSTATIC-PRESSURE, BACTERIAL CONSORTIUM, LACTOCOCCUS-LACTIS, HYDROTHERMAL-VENT, CRUDE-OIL, BIODEGRADATION, MICROORGANISMS, DEGRADATION, DIVERSITY, HALOMONAS

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MLA
Van Landuyt, Josefien, et al. “Microbial Enrichment, Functional Characterization and Isolation from a Cold Seep Yield Piezotolerant Obligate Hydrocarbon Degraders.” FEMS MICROBIOLOGY ECOLOGY, vol. 96, no. 9, 2020, doi:10.1093/femsec/fiaa097.
APA
Van Landuyt, J., Cimmino, L., Dumolin, C., Chatzigiannidou, I., Taveirne, F., Mattelin, V., … Boon, N. (2020). Microbial enrichment, functional characterization and isolation from a cold seep yield piezotolerant obligate hydrocarbon degraders. FEMS MICROBIOLOGY ECOLOGY, 96(9). https://doi.org/10.1093/femsec/fiaa097
Chicago author-date
Van Landuyt, Josefien, Lorenzo Cimmino, Charles Dumolin, Ioanna Chatzigiannidou, Felix Taveirne, Valérie Mattelin, Yu Zhang, et al. 2020. “Microbial Enrichment, Functional Characterization and Isolation from a Cold Seep Yield Piezotolerant Obligate Hydrocarbon Degraders.” FEMS MICROBIOLOGY ECOLOGY 96 (9). https://doi.org/10.1093/femsec/fiaa097.
Chicago author-date (all authors)
Van Landuyt, Josefien, Lorenzo Cimmino, Charles Dumolin, Ioanna Chatzigiannidou, Felix Taveirne, Valérie Mattelin, Yu Zhang, Peter Vandamme, Alberto Scoma, Adam Williamson, and Nico Boon. 2020. “Microbial Enrichment, Functional Characterization and Isolation from a Cold Seep Yield Piezotolerant Obligate Hydrocarbon Degraders.” FEMS MICROBIOLOGY ECOLOGY 96 (9). doi:10.1093/femsec/fiaa097.
Vancouver
1.
Van Landuyt J, Cimmino L, Dumolin C, Chatzigiannidou I, Taveirne F, Mattelin V, et al. Microbial enrichment, functional characterization and isolation from a cold seep yield piezotolerant obligate hydrocarbon degraders. FEMS MICROBIOLOGY ECOLOGY. 2020;96(9).
IEEE
[1]
J. Van Landuyt et al., “Microbial enrichment, functional characterization and isolation from a cold seep yield piezotolerant obligate hydrocarbon degraders,” FEMS MICROBIOLOGY ECOLOGY, vol. 96, no. 9, 2020.
@article{8678714,
  abstract     = {{Deep-sea environments can become contaminated with petroleum hydrocarbons. The effects of hydrostatic pressure (HP) in the deep sea on microbial oil degradation are poorly understood. Here, we performed long-term enrichments (100 days) from a natural cold seep while providing optimal conditions to sustain high hydrocarbon degradation rates. Through enrichments performed at increased HP and ambient pressure (AP) and by using control enrichments with marine broth, we demonstrated that both pressure and carbon source can have a big impact on the community structure. In contrast to previous studies, hydrocarbonoclastic operational taxonomic units (OTUs) remained dominant at both AP and increased HP, suggesting piezotolerance of these OTUs over the tested pressure range. Twenty-three isolates were obtained after isolation and dereplication. After recultivation at increased HP, an Alcanivorax sp. showed promising piezotolerance in axenic culture. Furthermore, preliminary co-cultivation tests indicated synergistic growth between some isolates, which shows promise for future synthetic community construction. Overall, more insights into the effect of increased HP on oil-degrading communities were obtained as well as several interesting isolates, e.g. a piezotolerant hydrocarbonoclastic bacterium for future deep-sea bioaugmentation investigation.}},
  articleno    = {{fiaa097}},
  author       = {{Van Landuyt, Josefien and Cimmino, Lorenzo and Dumolin, Charles and Chatzigiannidou, Ioanna and Taveirne, Felix and Mattelin, Valérie and Zhang, Yu and Vandamme, Peter and Scoma, Alberto and Williamson, Adam and Boon, Nico}},
  issn         = {{0168-6496}},
  journal      = {{FEMS MICROBIOLOGY ECOLOGY}},
  keywords     = {{bioaugmentation,oil degradation,microbial community,hydrocarbonoclastic,piezotolerance,deep-sea bacteria,HIGH HYDROSTATIC-PRESSURE,BACTERIAL CONSORTIUM,LACTOCOCCUS-LACTIS,HYDROTHERMAL-VENT,CRUDE-OIL,BIODEGRADATION,MICROORGANISMS,DEGRADATION,DIVERSITY,HALOMONAS}},
  language     = {{eng}},
  number       = {{9}},
  pages        = {{15}},
  title        = {{Microbial enrichment, functional characterization and isolation from a cold seep yield piezotolerant obligate hydrocarbon degraders}},
  url          = {{http://dx.doi.org/10.1093/femsec/fiaa097}},
  volume       = {{96}},
  year         = {{2020}},
}

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