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Antibiotic affects the gut microbiota composition and expression of genes related to lipid metabolism and myofiber types in skeletal muscle of piglets

Honglin Yan (UGent) , Bing Yu, Jeroen Degroote (UGent) , Thomas Spranghers (UGent) , Noémie Van Noten (UGent) , Maryam Majdeddin (UGent) , Mario Van Poucke (UGent) , Luc Peelman (UGent) , Jo De Vrieze (UGent) , Nico Boon (UGent) , et al.
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Abstract
Background: Early-life antibiotic administration is known to affect gut microbiota and host adiposity, but the effects of antibiotic exposure on skeletal muscle properties remain unknown. The present study evaluated the changes in skeletal muscle properties including myofiber characteristics and composition, as well as intramuscular fat (IMF) content in skeletal muscle of piglets when exposed to a tylosin-containing diet. Results: A total of 18 piglets (28 days of age) were randomly allocated into two groups: control basal diet (Control) and Control + 100 mg tylosin phosphate/kg of feed (Antibiotic). The trial lasted for 39 days. High-throughput amplicon sequencing revealed that no significant difference in initial gut microbiota composition was existed between Control and Antibiotic groups. Antibiotic administration increased body weight and growth rate and decreased feed to gain ratio of pigs (P < 0.05). The carcass lean and fat volumes of pigs were increased by the tylosin administration (P < 0.05). Antibiotic treatment increased myofiber density and the expression of genes related to type I and type IIb myofibers in longissimus muscle (P < 0.05). The IMF content in longissimus muscle was increased by antibiotic exposure (P < 0.05). Antibiotic administration increased expression of genes related to fatty acid uptake and de novo synthesis, and decreased expression of genes related to triglyceride hydrolysis (P < 0.05). Tylosin administration affected taxonomic distribution and beta diversity of the caecal and colonic microbiota of piglets. Conclusion: These results confirm that the growth performance, myofiber composition and muscle lipid metabolism are affected by antibiotic administration, which may be associated with an altered gut microbiota, suggesting that the gut microbiota could be served as a potential target for modulating skeletal muscle properties of host.
Keywords
General Veterinary, General Medicine, Tylosin, Intramuscular fat content, Lipid metabolism, Gut microbiota, Skeletal muscle, Piglets, FIBER CHARACTERISTICS, MEAT QUALITY, INTESTINAL MICROBIOTA, SIGNALING PATHWAYS, GROWTH-PERFORMANCE, LARGE WHITE, PIGS, DIGESTIBILITY, CARCASS, OBESITY

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MLA
Yan, Honglin, et al. “Antibiotic Affects the Gut Microbiota Composition and Expression of Genes Related to Lipid Metabolism and Myofiber Types in Skeletal Muscle of Piglets.” BMC VETERINARY RESEARCH, vol. 16, no. 1, 2020, doi:10.1186/s12917-020-02592-0.
APA
Yan, H., Yu, B., Degroote, J., Spranghers, T., Van Noten, N., Majdeddin, M., … Michiels, J. (2020). Antibiotic affects the gut microbiota composition and expression of genes related to lipid metabolism and myofiber types in skeletal muscle of piglets. BMC VETERINARY RESEARCH, 16(1). https://doi.org/10.1186/s12917-020-02592-0
Chicago author-date
Yan, Honglin, Bing Yu, Jeroen Degroote, Thomas Spranghers, Noémie Van Noten, Maryam Majdeddin, Mario Van Poucke, et al. 2020. “Antibiotic Affects the Gut Microbiota Composition and Expression of Genes Related to Lipid Metabolism and Myofiber Types in Skeletal Muscle of Piglets.” BMC VETERINARY RESEARCH 16 (1). https://doi.org/10.1186/s12917-020-02592-0.
Chicago author-date (all authors)
Yan, Honglin, Bing Yu, Jeroen Degroote, Thomas Spranghers, Noémie Van Noten, Maryam Majdeddin, Mario Van Poucke, Luc Peelman, Jo De Vrieze, Nico Boon, Ingrid Gielen, Stefaan De Smet, Daiwen Chen, and Joris Michiels. 2020. “Antibiotic Affects the Gut Microbiota Composition and Expression of Genes Related to Lipid Metabolism and Myofiber Types in Skeletal Muscle of Piglets.” BMC VETERINARY RESEARCH 16 (1). doi:10.1186/s12917-020-02592-0.
Vancouver
1.
Yan H, Yu B, Degroote J, Spranghers T, Van Noten N, Majdeddin M, et al. Antibiotic affects the gut microbiota composition and expression of genes related to lipid metabolism and myofiber types in skeletal muscle of piglets. BMC VETERINARY RESEARCH. 2020;16(1).
IEEE
[1]
H. Yan et al., “Antibiotic affects the gut microbiota composition and expression of genes related to lipid metabolism and myofiber types in skeletal muscle of piglets,” BMC VETERINARY RESEARCH, vol. 16, no. 1, 2020.
@article{8678091,
  abstract     = {{Background: Early-life antibiotic administration is known to affect gut microbiota and host adiposity, but the effects of antibiotic exposure on skeletal muscle properties remain unknown. The present study evaluated the changes in skeletal muscle properties including myofiber characteristics and composition, as well as intramuscular fat (IMF) content in skeletal muscle of piglets when exposed to a tylosin-containing diet.

Results: A total of 18 piglets (28 days of age) were randomly allocated into two groups: control basal diet (Control) and Control + 100 mg tylosin phosphate/kg of feed (Antibiotic). The trial lasted for 39 days. High-throughput amplicon sequencing revealed that no significant difference in initial gut microbiota composition was existed between Control and Antibiotic groups. Antibiotic administration increased body weight and growth rate and decreased feed to gain ratio of pigs (P < 0.05). The carcass lean and fat volumes of pigs were increased by the tylosin administration (P < 0.05). Antibiotic treatment increased myofiber density and the expression of genes related to type I and type IIb myofibers in longissimus muscle (P < 0.05). The IMF content in longissimus muscle was increased by antibiotic exposure (P < 0.05). Antibiotic administration increased expression of genes related to fatty acid uptake and de novo synthesis, and decreased expression of genes related to triglyceride hydrolysis (P < 0.05). Tylosin administration affected taxonomic distribution and beta diversity of the caecal and colonic microbiota of piglets.

Conclusion: These results confirm that the growth performance, myofiber composition and muscle lipid metabolism are affected by antibiotic administration, which may be associated with an altered gut microbiota, suggesting that the gut microbiota could be served as a potential target for modulating skeletal muscle properties of host.}},
  articleno    = {{392}},
  author       = {{Yan, Honglin and Yu, Bing and Degroote, Jeroen and Spranghers, Thomas and Van Noten, Noémie and Majdeddin, Maryam and Van Poucke, Mario and Peelman, Luc and De Vrieze, Jo and Boon, Nico and Gielen, Ingrid and De Smet, Stefaan and Chen, Daiwen and Michiels, Joris}},
  issn         = {{1746-6148}},
  journal      = {{BMC VETERINARY RESEARCH}},
  keywords     = {{General Veterinary,General Medicine,Tylosin,Intramuscular fat content,Lipid metabolism,Gut microbiota,Skeletal muscle,Piglets,FIBER CHARACTERISTICS,MEAT QUALITY,INTESTINAL MICROBIOTA,SIGNALING PATHWAYS,GROWTH-PERFORMANCE,LARGE WHITE,PIGS,DIGESTIBILITY,CARCASS,OBESITY}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{12}},
  title        = {{Antibiotic affects the gut microbiota composition and expression of genes related to lipid metabolism and myofiber types in skeletal muscle of piglets}},
  url          = {{http://dx.doi.org/10.1186/s12917-020-02592-0}},
  volume       = {{16}},
  year         = {{2020}},
}

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