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Development of a novel and rapid phenotype-based screening method to assess rice seedling growth

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Abstract
<jats:title>Abstract</jats:title> <jats:sec> <jats:title>Background</jats:title> <jats:p>Rice (<jats:italic>Oryza sativa</jats:italic>) is one of the most important model crops in plant research. Despite its considerable advantages, (phenotypic) bioassays for rice are not as well developed as for <jats:italic>Arabidopsis thaliana</jats:italic>. Here, we present a phenotype-based screening method to study shoot-related parameters of rice seedlings via an automated computer analysis.</jats:p> </jats:sec> <jats:sec> <jats:title>Results</jats:title> <jats:p>The phenotype-based screening method was validated by testing several compounds in pharmacological experiments that interfered with hormone homeostasis, confirming that the assay was consistent with regard to the anticipated plant growth regulation and revealing the robustness of the set-up in terms of reproducibility. Moreover, abiotic stress tests using NaCl and DCMU, an electron transport blocker during the light dependent reactions of photosynthesis, confirmed the validity of the new method for a wide range of applications. Next, this method was used to screen the impact of semi-purified fractions of marine invertebrates on the initial stages of rice seedling growth. Certain fractions clearly stimulated growth, whereas others inhibited it, especially in the root, illustrating the possible applications of this novel, robust, and fast phenotype-based screening method for rice.</jats:p> </jats:sec> <jats:sec> <jats:title>Conclusions</jats:title> <jats:p>The validated phenotype-based and cost-efficient screening method allows a quick and proper analysis of shoot growth and requires only small volumes of compounds and media. As a result, this method could potentially be used for a whole range of applications, ranging from discovery of novel biostimulants, plant growth regulators, and plant growth-promoting bacteria to analysis of CRISPR knockouts, molecular plant breeding, genome-wide association, and phytotoxicity studies. The assay system described here can contribute to a better understanding of plant development in general.</jats:p> </jats:sec>
Keywords
Biotechnology, Plant Science, Genetics

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MLA
Vlaminck, Lena, et al. “Development of a Novel and Rapid Phenotype-Based Screening Method to Assess Rice Seedling Growth.” Plant Methods, vol. 16, 2020, doi:10.1186/s13007-020-00682-6.
APA
Vlaminck, L., Sang-aram, C., Botterman, D., Uy, C., Harper, M. K., Inzé, D., … Depuydt, S. (2020). Development of a novel and rapid phenotype-based screening method to assess rice seedling growth. Plant Methods, 16. https://doi.org/10.1186/s13007-020-00682-6
Chicago author-date
Vlaminck, Lena, Chananchida Sang-aram, Deborah Botterman, Christine Uy, Mary Kay Harper, Dirk Inzé, Godelieve Gheysen, and Stephen Depuydt. 2020. “Development of a Novel and Rapid Phenotype-Based Screening Method to Assess Rice Seedling Growth.” Plant Methods 16. https://doi.org/10.1186/s13007-020-00682-6.
Chicago author-date (all authors)
Vlaminck, Lena, Chananchida Sang-aram, Deborah Botterman, Christine Uy, Mary Kay Harper, Dirk Inzé, Godelieve Gheysen, and Stephen Depuydt. 2020. “Development of a Novel and Rapid Phenotype-Based Screening Method to Assess Rice Seedling Growth.” Plant Methods 16. doi:10.1186/s13007-020-00682-6.
Vancouver
1.
Vlaminck L, Sang-aram C, Botterman D, Uy C, Harper MK, Inzé D, et al. Development of a novel and rapid phenotype-based screening method to assess rice seedling growth. Plant Methods. 2020;16.
IEEE
[1]
L. Vlaminck et al., “Development of a novel and rapid phenotype-based screening method to assess rice seedling growth,” Plant Methods, vol. 16, 2020.
@article{8677995,
  abstract     = {<jats:title>Abstract</jats:title>
              <jats:sec>
                <jats:title>Background</jats:title>
                <jats:p>Rice (<jats:italic>Oryza sativa</jats:italic>) is one of the most important model crops in plant research. Despite its considerable advantages, (phenotypic) bioassays for rice are not as well developed as for <jats:italic>Arabidopsis thaliana</jats:italic>. Here, we present a phenotype-based screening method to study shoot-related parameters of rice seedlings via an automated computer analysis.</jats:p>
              </jats:sec>
              <jats:sec>
                <jats:title>Results</jats:title>
                <jats:p>The phenotype-based screening method was validated by testing several compounds in pharmacological experiments that interfered with hormone homeostasis, confirming that the assay was consistent with regard to the anticipated plant growth regulation and revealing the robustness of the set-up in terms of reproducibility. Moreover, abiotic stress tests using NaCl and DCMU, an electron transport blocker during the light dependent reactions of photosynthesis, confirmed the validity of the new method for a wide range of applications. Next, this method was used to screen the impact of semi-purified fractions of marine invertebrates on the initial stages of rice seedling growth. Certain fractions clearly stimulated growth, whereas others inhibited it, especially in the root, illustrating the possible applications of this novel, robust, and fast phenotype-based screening method for rice.</jats:p>
              </jats:sec>
              <jats:sec>
                <jats:title>Conclusions</jats:title>
                <jats:p>The validated phenotype-based and cost-efficient screening method allows a quick and proper analysis of shoot growth and requires only small volumes of compounds and media. As a result, this method could potentially be used for a whole range of applications, ranging from discovery of novel biostimulants, plant growth regulators, and plant growth-promoting bacteria to analysis of CRISPR knockouts, molecular plant breeding, genome-wide association, and phytotoxicity studies. The assay system described here can contribute to a better understanding of plant development in general.</jats:p>
              </jats:sec>},
  articleno    = {139},
  author       = {Vlaminck, Lena and Sang-aram, Chananchida and Botterman, Deborah and Uy, Christine and Harper, Mary Kay and Inzé, Dirk and Gheysen, Godelieve and Depuydt, Stephen},
  issn         = {1746-4811},
  journal      = {Plant Methods},
  keywords     = {Biotechnology,Plant Science,Genetics},
  language     = {eng},
  title        = {Development of a novel and rapid phenotype-based screening method to assess rice seedling growth},
  url          = {http://dx.doi.org/10.1186/s13007-020-00682-6},
  volume       = {16},
  year         = {2020},
}

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