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Mating type specific transcriptomic response to sex inducing pheromone in the pennate diatom Seminavis robusta

Gust Bilcke (UGent) , Koen Van den Berge (UGent) , Sam De Decker, Eli Bonneure (UGent) , Nicole Poulsen, Petra Bulánková (UGent) , Cristina Osuna, Jack Dickenson, Koen Sabbe (UGent) , Georg Pohnert, et al.
(2021) ISME JOURNAL. 15. p.562-576
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Abstract
Sexual reproduction is a fundamental phase in the life cycle of most diatoms. Despite its role as a source of genetic variation, it is rarely reported in natural circumstances and its molecular foundations remain largely unknown. Here, we integrate independent transcriptomic datasets to prioritize genes responding to sex inducing pheromones (SIPs) in the pennate diatomSeminavis robusta. We observe marked gene expression changes associated with SIP treatment in both mating types, including an inhibition of S phase progression, chloroplast division, mitosis, and cell wall formation. Meanwhile, meiotic genes are upregulated in response to SIP, including a sexually induced diatom specific cyclin. Our data further suggest an important role for reactive oxygen species, energy metabolism, and cGMP signaling during the early stages of sexual reproduction. In addition, we identify several genes with a mating type specific response to SIP, and link their expression pattern with physiological specialization, such as the production of the attraction pheromone diproline in mating type - (MT-) and mate-searching behavior in mating type + (MT+). Combined, our results provide a model for early sexual reproduction in pennate diatoms and significantly expand the suite of target genes to detect sexual reproduction events in natural diatom populations.
Keywords
Ecology, Evolution, Behavior and Systematics, Microbiology, UNICELLULAR CHAROPHYCEAN ALGA, GENE-EXPRESSION, CELL-CYCLE, REPRODUCTION, PROTEIN, FAMILY, EVOLUTION, DIVISION, DIATOMS, IDENTIFICATION

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MLA
Bilcke, Gust, et al. “Mating Type Specific Transcriptomic Response to Sex Inducing Pheromone in the Pennate Diatom Seminavis Robusta.” ISME JOURNAL, vol. 15, 2021, pp. 562–76, doi:10.1038/s41396-020-00797-7.
APA
Bilcke, G., Van den Berge, K., De Decker, S., Bonneure, E., Poulsen, N., Bulánková, P., … Vyverman, W. (2021). Mating type specific transcriptomic response to sex inducing pheromone in the pennate diatom Seminavis robusta. ISME JOURNAL, 15, 562–576. https://doi.org/10.1038/s41396-020-00797-7
Chicago author-date
Bilcke, Gust, Koen Van den Berge, Sam De Decker, Eli Bonneure, Nicole Poulsen, Petra Bulánková, Cristina Osuna, et al. 2021. “Mating Type Specific Transcriptomic Response to Sex Inducing Pheromone in the Pennate Diatom Seminavis Robusta.” ISME JOURNAL 15: 562–76. https://doi.org/10.1038/s41396-020-00797-7.
Chicago author-date (all authors)
Bilcke, Gust, Koen Van den Berge, Sam De Decker, Eli Bonneure, Nicole Poulsen, Petra Bulánková, Cristina Osuna, Jack Dickenson, Koen Sabbe, Georg Pohnert, Klaas Vandepoele, Sven Mangelinckx, Lieven Clement, Lieven De Veylder, and Wim Vyverman. 2021. “Mating Type Specific Transcriptomic Response to Sex Inducing Pheromone in the Pennate Diatom Seminavis Robusta.” ISME JOURNAL 15: 562–576. doi:10.1038/s41396-020-00797-7.
Vancouver
1.
Bilcke G, Van den Berge K, De Decker S, Bonneure E, Poulsen N, Bulánková P, et al. Mating type specific transcriptomic response to sex inducing pheromone in the pennate diatom Seminavis robusta. ISME JOURNAL. 2021;15:562–76.
IEEE
[1]
G. Bilcke et al., “Mating type specific transcriptomic response to sex inducing pheromone in the pennate diatom Seminavis robusta,” ISME JOURNAL, vol. 15, pp. 562–576, 2021.
@article{8677906,
  abstract     = {{Sexual reproduction is a fundamental phase in the life cycle of most diatoms. Despite its role as a source of genetic variation, it is rarely reported in natural circumstances and its molecular foundations remain largely unknown. Here, we integrate independent transcriptomic datasets to prioritize genes responding to sex inducing pheromones (SIPs) in the pennate diatomSeminavis robusta. We observe marked gene expression changes associated with SIP treatment in both mating types, including an inhibition of S phase progression, chloroplast division, mitosis, and cell wall formation. Meanwhile, meiotic genes are upregulated in response to SIP, including a sexually induced diatom specific cyclin. Our data further suggest an important role for reactive oxygen species, energy metabolism, and cGMP signaling during the early stages of sexual reproduction. In addition, we identify several genes with a mating type specific response to SIP, and link their expression pattern with physiological specialization, such as the production of the attraction pheromone diproline in mating type - (MT-) and mate-searching behavior in mating type + (MT+). Combined, our results provide a model for early sexual reproduction in pennate diatoms and significantly expand the suite of target genes to detect sexual reproduction events in natural diatom populations.}},
  author       = {{Bilcke, Gust and Van den Berge, Koen and De Decker, Sam and Bonneure, Eli and Poulsen, Nicole and Bulánková, Petra and Osuna, Cristina and Dickenson, Jack and Sabbe, Koen and Pohnert, Georg and Vandepoele, Klaas and Mangelinckx, Sven and Clement, Lieven and De Veylder, Lieven and Vyverman, Wim}},
  issn         = {{1751-7362}},
  journal      = {{ISME JOURNAL}},
  keywords     = {{Ecology,Evolution,Behavior and Systematics,Microbiology,UNICELLULAR CHAROPHYCEAN ALGA,GENE-EXPRESSION,CELL-CYCLE,REPRODUCTION,PROTEIN,FAMILY,EVOLUTION,DIVISION,DIATOMS,IDENTIFICATION}},
  language     = {{eng}},
  pages        = {{562--576}},
  title        = {{Mating type specific transcriptomic response to sex inducing pheromone in the pennate diatom Seminavis robusta}},
  url          = {{http://doi.org/10.1038/s41396-020-00797-7}},
  volume       = {{15}},
  year         = {{2021}},
}
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