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Hydrophilic interaction LC of peptides: columns comparison and clustering

Sylvia Van Dorpe UGent, Valentijn Vergote UGent, Adel Pezeshki UGent, Christian Burvenich UGent, Kathelijne Peremans UGent and Bart De Spiegeleer UGent (2010) JOURNAL OF SEPARATION SCIENCE. 33(6-7). p.728-739
abstract
A wide variety of hydrophilic interaction chromatography (HILIC) stationary phase surface chemistries are currently available. Although their selectivity can be considerably different, column comparison or clustering using peptides is limited. In this study, ten pharmaceutically relevant model peptides are analyzed on seven different HILIC columns (bare silica, amide, poly-hydroxyethyl aspartamide, diol and zwitterionic) for the evaluation of their performance and classification. The responses examined include single and multiple responses: plate number, asymmetry factor, LOD, geometric mean resolution, resolution product, time corrected resolution product, peak capacity and chromatographic response function. Column classification was performed using hierarchical clustering and principal component analysis. Moreover, the overall performance quality of the HILIC columns was compared using a linear desirability function. Hierarchical clustering and principal component analysis showed consistent clusters. The zwitterionic phase was clustered apart from the other HILIC columns and both poly-aspartamide columns were clustered together. In addition, the two bare silica phases represent two different clusters, and thus different selectivities. Overall, the responses showed the best performance for one of the bare silica columns (Alltima-Alltech), followed by the zwitterionic phase (ZIC)-HILIC. Thus, these columns, belonging to different clusters, were found to be the best performing systems in pharmaceutical peptide analysis for the selected peptide set.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
Peptides, Classification, Stationary phase, HILIC
journal title
JOURNAL OF SEPARATION SCIENCE
J. Sep. Sci.
editor
Michael Lämmerhofer
volume
33
issue
6-7
issue title
HILIC and Mixed Modes
pages
728 - 739
Web of Science type
article
Web of Science id
000276782700005
JCR category
CHEMISTRY, ANALYTICAL
JCR impact factor
2.631 (2010)
JCR rank
23/71 (2010)
JCR quartile
2 (2010)
ISSN
1615-9306
DOI
10.1002/jssc.200900476
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
867751
handle
http://hdl.handle.net/1854/LU-867751
alternative location
http://www3.interscience.wiley.com/journal/123274173/abstract
date created
2010-02-18 14:13:14
date last changed
2010-06-17 16:28:09
@article{867751,
  abstract     = {A wide variety of hydrophilic interaction chromatography (HILIC) stationary phase surface chemistries are currently available. Although their selectivity can be considerably different, column comparison or clustering using peptides is limited. In this study, ten pharmaceutically relevant model peptides are analyzed on seven different HILIC columns (bare silica, amide, poly-hydroxyethyl aspartamide, diol and zwitterionic) for the evaluation of their performance and classification. The responses examined include single and multiple responses: plate number, asymmetry factor, LOD, geometric mean resolution, resolution product, time corrected resolution product, peak capacity and chromatographic response function. Column classification was performed using hierarchical clustering and principal component analysis. Moreover, the overall performance quality of the HILIC columns was compared using a linear desirability function. Hierarchical clustering and principal component analysis showed consistent clusters. The zwitterionic phase was clustered apart from the other HILIC columns and both poly-aspartamide columns were clustered together. In addition, the two bare silica phases represent two different clusters, and thus different selectivities. Overall, the responses showed the best performance for one of the bare silica columns (Alltima-Alltech), followed by the zwitterionic phase (ZIC)-HILIC. Thus, these columns, belonging to different clusters, were found to be the best performing systems in pharmaceutical peptide analysis for the selected peptide set.},
  author       = {Van Dorpe, Sylvia and Vergote, Valentijn and Pezeshki, Adel and Burvenich, Christian and Peremans, Kathelijne and De Spiegeleer, Bart},
  editor       = {L{\"a}mmerhofer, Michael},
  issn         = {1615-9306},
  journal      = {JOURNAL OF SEPARATION SCIENCE},
  keyword      = {Peptides,Classification,Stationary phase,HILIC},
  language     = {eng},
  number       = {6-7},
  pages        = {728--739},
  title        = {Hydrophilic interaction LC of peptides: columns comparison and clustering},
  url          = {http://dx.doi.org/10.1002/jssc.200900476},
  volume       = {33},
  year         = {2010},
}

Chicago
Van Dorpe, Sylvia, Valentijn Vergote, Adel Pezeshki, Christian Burvenich, Kathelijne Peremans, and Bart De Spiegeleer. 2010. “Hydrophilic Interaction LC of Peptides: Columns Comparison and Clustering.” Ed. Michael Lämmerhofer. Journal of Separation Science 33 (6-7): 728–739.
APA
Van Dorpe, S., Vergote, V., Pezeshki, A., Burvenich, C., Peremans, K., & De Spiegeleer, B. (2010). Hydrophilic interaction LC of peptides: columns comparison and clustering. (M. Lämmerhofer, Ed.)JOURNAL OF SEPARATION SCIENCE, 33(6-7), 728–739.
Vancouver
1.
Van Dorpe S, Vergote V, Pezeshki A, Burvenich C, Peremans K, De Spiegeleer B. Hydrophilic interaction LC of peptides: columns comparison and clustering. Lämmerhofer M, editor. JOURNAL OF SEPARATION SCIENCE. 2010;33(6-7):728–39.
MLA
Van Dorpe, Sylvia, Valentijn Vergote, Adel Pezeshki, et al. “Hydrophilic Interaction LC of Peptides: Columns Comparison and Clustering.” Ed. Michael Lämmerhofer. JOURNAL OF SEPARATION SCIENCE 33.6-7 (2010): 728–739. Print.